Sensitivity and specificity of hr hPV E6/E7 mrna test in detecting cervical squamous intraepithelial lesion and cervical cancer

Abstract

Objectives: The paper assess the relevance of HR HPV E6/E7 mRNA test in women with abnormal Pap results. Material and methods: Between 2013-2014, 125 women were subjects to the enhanced diagnostics due to abnormal Pap results. According to The Bethesda system, if ASC-US, AGC, LSIL, ASC-H, HSIL or cancer cells were present, the result was abnormal. The patients underwent the enhanced diagnostics which included the following procedures: Pap smear collection for molecular assessment of HR HPV E6/E7 mRNA test, the colposcopic examination and biopsy of clinically suspicious areas. Results: High-grade squamous intraepithelial lesions constituted the most frequent cervical pathology in women with abnormal Pap test results, as well as with the positive results of HR HPV E6/E7 mRNA test. Test sensitivity in patients with the histopathological diagnosis of high-grade squamous intraepithelial lesion was estimated at 86.1%. Conclusions: HR HPV E6/E7 mRNA test identifying neoplastic lesions and cervical cancer is characterised by a high relevance which is reflected by means of sensitivity and specificity. In fact, test sensitivity and specificity increased with the age in the group of patients up to 50 years old.

Diagnostic value of high-risk HPV E6/E7 mRNA in patients with ASCUS Analysis of pathogenicity of HPV in patients with ASCUS

“…The detection rate of HSIL + in the HPV16 group was 48.4%, which was signi cantly higher than that of the 18.8% in the HPV18/45 + group; that is, the highgrade (HSIL+) morbidity of HPV16/18/45 to the cervix was signi cantly higher than that of the Other HR-HPV, while HPV16 was signi cantly higher than HPV18/45. Pruski et al [22] found that high-grade squamous intraepithelial lesions were closely related to the positive expression of HPV E6/E7 mRNA. HPV-16 is the most common genotype in CIN2 + lesions diagnosed by LSIL in Chinese women, while HPV-18 is the most common genotype in CIN1 lesions [23], which is basically consistent with this conclusion.…”

Tao brush endometrial cytology is a sensitive diagnostic tool for cancer and hyperplasia among women presenting to clinic with abnormal uterine bleeding

“…Cervical cytology techniques continue to evolve with the incorporation of automation, artificial intelligence, and ancillary molecular tests (such as HPV) that have improved test performance. 23 , 31 , 32 , 33 , 34 Likewise, endometrial cytology techniques are ripe for ancillary test development to enhance or replace manual cytomorphologic evaluation, but technology has yet to be developed or tested. For now, inter‐observer variability is a factor in the reading of endometrial cytology by pathologists and likely would require specialized training for those who would read this type of sample regularly.…”

The diagnostic value of HPV E6/E7 mRNA test in young women with cervical squamous intraepithelial lesion: A Retrospective Analysis

“…There are many studies on the performance of HPV E6/E7 mRNA test in diagnosing cervical diseases. Some reports have suggested that HPV E6 / E7 mRNA test is more sensitive and speci c than the HPV NDA test [22][23][24]. It has also been suggested that the HPV E6/E7 mRNA test is only more speci c than the HPV DNA test,having no difference in sensitivity [25].…”