Sensitivity of bacterial biofilms and planktonic cells to a new antimicrobial agent, Oxsil® 320N

Surdeau, N.; Laurent-Maquin, Dominique; Bouthors, S.; Gellé, Marie-Paule

doi:10.1016/j.jhin.2005.09.003

Cited by 38 publications

(18 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Bacteria were collected from glass slides by mechanical agitation in buffered peptone water. These soft sonication and vortex procedures were previously demonstrated to be without effect on bacteria viability [27]. Both bacterial dilution and seeding on nutrient agar plates were performed with EasySpiral Pro® (Interscience, France).…”

Section: Methodsmentioning

confidence: 99%

Innovative non-thermal plasma disinfection process inside sealed bags: Assessment of bactericidal and sporicidal effectiveness in regard to current sterilization norms

et al. 2017

View full text Add to dashboard Cite

In this work, we developed a device capable to generate a non-thermal plasma discharge inside a sealed bag. The aim of this study was to assess the effectiveness of the oxygen, nitrogen and argon plasma sterilization on Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis spores according to the NF EN 556 Norm. Moreover the bag integrity which is a critical key to maintain the sterile state of items after the end of the process was verified by Fourier Transform Infrared (FTIR) and X-ray Photoelectron Spectrometry (XPS) analyses. After plasma treatments, the bacterial counting showed a 6 log reduction of P. aeruginosa and S. aureus in 45 min and 120 min respectively whatever the gas used and a 4 log reduction of B. subtilis spores in 120 min with only oxygen plasma. These results were confirmed by Scanning Electron Microscopy (SEM) observations showing altered bacteria or spores and numerous debris. Taking into account the studied microorganisms, the oxygen plasma treatment showed the highest efficiency. FTIR and XPS analyses showed that this treatment induced no significant modification of the bags. To conclude this non-thermal plasma sterilization technique could be an opportunity to sterilize heat and chemical-sensitive medical devices and to preserve their sterile state after the end of the process.

show abstract

Section: Methodsmentioning

confidence: 99%

Innovative non-thermal plasma disinfection process inside sealed bags: Assessment of bactericidal and sporicidal effectiveness in regard to current sterilization norms

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Artificial P. aeruginosa biofilms resisted treatment with various biocidal agents including peracetic acid, compared with their planktonic counterparts [150][151][152] . Biofilms composed of E. coli [152,153] , S. aureus [152,154,155] , Mycobacterium fortuitum [156] or Listeria monocytogenes [157] also resisted treatment with diverse biocides compared with planktonic cells. Bacteria in mature (old) biofilms were more resistant to killing than those in young biofilms [153,158,159] .…”

Section: Resistance Of Biofilm Bacteriamentioning

confidence: 99%

Is peracetic acid suitable for the cleaning step of reprocessing flexible endoscopes?

Kampf

Fliss

Martiny

2014

WJGE

View full text Add to dashboard Cite

The bioburden (blood, protein, pathogens and biofilm) on flexible endoscopes after use is often high and its removal is essential to allow effective disinfection, especially in the case of peracetic acid-based disinfectants, which are easily inactivated by organic material. Cleaning processes using conventional cleaners remove a variable but often sufficient amount of the bioburden. Some formulations based on peracetic acid are recommended by manufacturers for the cleaning step. We performed a systematic literature search and reviewed the available evidence to clarify the suitability of peracetic acid-based formulations for cleaning flexible endoscopes. A total of 243 studies were evaluated. No studies have yet demonstrated that peracetic acidbased cleaners are as effective as conventional cleaners. Some peracetic acid-based formulations have demonstrated some biofilm-cleaning effects and no biofilmfixation potential, while others have a limited cleaning effect and a clear biofilm-fixation potential. All published data demonstrated a limited blood cleaning effect and a substantial blood and nerve tissue fixation potential of peracetic acid. No evidence-based guidelines on reprocessing flexible endoscopes currently recommend using cleaners containing peracetic acid, but some guidelines clearly recommend not using them because of their fixation potential. Evidence from some outbreaks, especially those involving highly multidrug-resistant gram-negative pathogens, indicated that disinfection using peracetic acid may be insufficient if the preceding cleaning step is not performed adequately. Based on this review we conclude that peracetic acid-based formulations should not be used for cleaning flexible endoscopes. Key words: Peracetic acid; Cleaning; Flexible endoscope; Biofilm; Resistance; Bioburden; Blood; Disinfection; Reprocessing Core tip: Some formulations based on peracetic acid (PAA) are recommended by manufacturers for cleaning flexible endoscopes. We reviewed 243 studies to analyse the evidence for this recommendation. No study demonstrated that PAA-based cleaners were as effective as conventional cleaners, and some PAA-based formulations had clear biofilm-fixation potential. Dried blood and nerve tissue were substantially fixed by PAA. Some outbreaks, especially of highly multidrug-resistant gram-negative pathogens, indicated that insufficient cleaning could not be compensated for by using PAA in the disinfection step. PAA-based formulations should not be used for cleaning flexible endoscopes.

show abstract

“…When biofilms were exposed to Oxsil® 320N, the concentration needed to achieve a 10 5 -fold decrease in concentration was ten times higher than that for bacterial suspensions (0.313% Oxsil® 320N). An effective concentration of Oxsil® 320N of 3.13% was required (Surdeau et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Control of Microbial Adhesion as a Strategy for Food and Bioprocess Technology

Araújo

Andrade

Silva

et al. 2009

Food Bioprocess Technol

View full text Add to dashboard Cite

Bacteria and other microorganisms have a natural tendency to adhere to surfaces as a survival mechanism. This can occur in many environments, including the living host, industrial systems, and natural waters. The general outcome of bacterial colonization of a surface is biofilm formation, which consists of microorganisms immobilized in a variety of polymeric compounds generally referred to as extracellular polymeric substances. Bacterial adhesion to a solid surface is a crucial step in the biofilm process. This step is dependent upon van der Waals, electrostatic, and acid-base interactions. These interactions are influenced by physicochemical properties of the substratum and the bacterial surface, such as hydrophobicity, surface charge, and electron donor-electron acceptor properties. In addition, the roughness of the substratum and the microbiological characteristics of the cell surface, such as cellular appendages and production of exopolysaccharides, can affect the adherence process. To date, many strategies have been developed to decrease the adherence of bacteria to surfaces. Surface modification with the addition of the suitable compounds makes surfaces less attractive for microorganisms and therefore prevents bacterial adherence and biofilm formation.

show abstract

Sensitivity of bacterial biofilms and planktonic cells to a new antimicrobial agent, Oxsil® 320N

Cited by 38 publications

References 22 publications

Innovative non-thermal plasma disinfection process inside sealed bags: Assessment of bactericidal and sporicidal effectiveness in regard to current sterilization norms

Innovative non-thermal plasma disinfection process inside sealed bags: Assessment of bactericidal and sporicidal effectiveness in regard to current sterilization norms

Is peracetic acid suitable for the cleaning step of reprocessing flexible endoscopes?

Control of Microbial Adhesion as a Strategy for Food and Bioprocess Technology

Contact Info

Product

Resources

About