2001
DOI: 10.1006/abbi.2001.2533
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Sensitivity of Deinococcus radiodurans to γ-Irradiation: A Novel Approach by Fourier Transform Infrared Spectroscopy

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Cited by 21 publications
(9 citation statements)
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“…Bands comprising Amide I are primarily formed by the contributions of C ϭ O bond vibrations in amide groups, and can provide information on changes in protein secondary structure (Fabian and M ä ntele 2002). Th e changes in these bands are suggestive of severe oxidative modifi cations of amino acid side chains, conformational stability alteration and/or aggregation of proteins such as β sheet structures (Gault et al 2007) Previous FTIR studies of irradiated cells and organisms have also reported changes in absorbance intensities of Amide I bands following irradiation (Melin et al 2001, Gault et al 2005, Meade et al 2010. Th e authors attributed these decreases to the damaging eff ects of ionising radiation, particularly the generation of free radicals.…”
Section: Discussionmentioning
confidence: 96%
See 1 more Smart Citation
“…Bands comprising Amide I are primarily formed by the contributions of C ϭ O bond vibrations in amide groups, and can provide information on changes in protein secondary structure (Fabian and M ä ntele 2002). Th e changes in these bands are suggestive of severe oxidative modifi cations of amino acid side chains, conformational stability alteration and/or aggregation of proteins such as β sheet structures (Gault et al 2007) Previous FTIR studies of irradiated cells and organisms have also reported changes in absorbance intensities of Amide I bands following irradiation (Melin et al 2001, Gault et al 2005, Meade et al 2010. Th e authors attributed these decreases to the damaging eff ects of ionising radiation, particularly the generation of free radicals.…”
Section: Discussionmentioning
confidence: 96%
“…FTIR has been used to detect ionising radiation-induced biochemical changes in Deinococcus radiodurans bacteria (Melin et al 2001), human keratinocyte (HaCaT) cells (Gault et al 2005, Meade et al 2010, and human lymphocytes (Gault and Lefaix 2003). To our knowledge, this is the fi rst synchrotron FTIR study to examine the response of tumour tissue to MRT at a chemical level, thus providing novel information on potential eff ects of MRT and BB on proteins, lipids and nucleic acids within the tumour.…”
Section: Introductionmentioning
confidence: 98%
“…These investigations have since evolved to Raman spectroscopic studies of the effects of in-vitro proton irradiation of excised tissues [52], and radiotherapeutically irradiated (γ-radiation) tissue specimens [53][54][55]. Other studies with bacteria have investigated the effects of γ-radiation on Deinococcus radiodurans [56], Kocuria rosea [57]and Micrococcus luteus [58] with FTIR spectroscopy. Recently Gault et al have demonstrated the sensitivity of FTIR spectroscopy to apoptotic effects in γ-irradiated lymphocytes [59] and to molecular changes in HaCaT cells exposed to both γ-radiation and hydrogen peroxide [60], together with its sensitivity to biochemical alterations in HaCaT cells irradiated with α-particles delivered by micro-beams [61].…”
Section: Introductionmentioning
confidence: 99%
“…16), which indicated that the uranyl ions bound to the oxygen atom of carbonyl group or nitrogen atom of peptide bond of the BSA. These results also suggested that the uranyl ions promoted the α -helix changing to β -fold under pH 3.0 [12][13][14][15]. Zhou et al [16] have identified that the Mg 2+ , Cu 2+ , Zn 2+ induced the β -fold formation when interacting with BSA..…”
Section: Ftir Resultsmentioning
confidence: 77%