Sensitivity of different rat tumour strains to photodynamic treatment with chlorin e6

Kostenich, Genady; Zhuravkin, Ivan N.; Furmanchuk, A.V.; Zhavrid, Edvard

doi:10.1016/1011-1344(93)80012-x

Cited by 12 publications

(5 citation statements)

References 19 publications

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“…Thus, the cell membrane is very likely the principal target for Ce6 35. Third, animal studies36 found Ce6 to be a useful photosensitizer with low toxicity and preferential tumor localization, which could further reduce nonspecific damage to the normal cells.…”

Section: Resultsmentioning

confidence: 99%

Photosensitizer–Gold Nanorod Composite for Targeted Multimodal Therapy

Wang

You

Zhu

et al. 2013

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In this work, a DNA inter-strand replacement strategy for therapeutic activity has been successfully designed for multimodal therapy. In this multimodal therapy, chlorin e6 (Ce6) photosensitizer molecules are used for photodynamic therapy (PDT), while aptamer-AuNRs, are used for selective binding to target cancer cells and for photothermal therapy (PTT) with near infrared (NIR) laser irradiation. Aptamer Sgc8, which specifically targets leukemia T cells, is conjugated to an AuNR by a thiol-Au covalent bond. and then hybridized with a Ce6-labeled photosensitizer/reporter to form a DNA double helix. When target cancer cells are absent, Ce6 is quenched and shows no PDT effect. However, when target cancer cells are present, the aptamer changes structure to release Ce6 to produce singlet oxygen for PDT upon light irradiation. Importantly, by combining photosensitizer and photothermal agents, PTT/PDT dual therapy supplies a more effective therapeutic outcome than either therapeutic modality alone.

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Section: Resultsmentioning

confidence: 99%

Photosensitizer–Gold Nanorod Composite for Targeted Multimodal Therapy

Wang

You

Zhu

et al. 2013

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116

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“…It appears that additional factors contribute to HY‐PDT–induced tumor damage in vivo and that the observed phenomena must be explained also in terms of biological arguments. It has been shown that the structural–functional characteristics of tumor microcirculation in different tumor strains may influence the response to PDT (28). We suggest that in some tumor models, damage to the blood vessels in the peripheral area is critical.…”

Section: Discussionmentioning

confidence: 99%

Wavelength-dependent Properties of Photodynamic Therapy Using Hypericin in vitro and in an Animal Model¶

Blank

Kostenich²,

Lavie

et al. 2007

Photochemistry and Photobiology

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Wavelength effects in photodynamic therapy (PDT) with hypericin (HY) were examined in a C26 colon carcinoma model both in vitro and in vivo. Irradiation of HY‐sensitized cells in vitro with either 550 or 590 nm caused the loss of cell viability in a drug‐ and light‐dose–dependent manner. The calculated ratio of HY‐based PDT (HY‐PDT) efficiencies at these two wavelengths was found to correlate with the numerical ratio of absorbed photons at each wavelength. In vivo irradiation of C26‐derived tumors, 6 h after intraperitoneal administration of HY (5 mg/kg), caused extensive vascular damage and tumor necrosis. The depth of tumor necrosis (d) was more pronounced at 590 than at 550 nm and increased when the light dose was raised from 60 to 120 J/cm2. The maximal depths of tumor necrosis (at 120 J/cm2) were 7.5 ± 1.5 mm at 550 nm and 9.9 ± 0.8 mm at 590 nm. Both values are rather high in view of the limited penetration of green‐yellow light into the tissue. Moreover, the depth ratio, d590/d550= 1.3 (P < 0.001), is smaller than expected considering the 2.2‐fold lower HY absorbance and the 1.7‐fold lower tissue penetration of radiation at 550 than at 590 nm. This finding indicates that in vivo the depth at which HY‐PDT elicits tumor necrosis is not only determined by photophysical considerations (light penetration, number of absorbed photons) but is also influenced significantly by other mechanisms such as vascular effects. Therefore, despite the relatively short‐wavelength peaks of absorption, our observations suggest that HY is an effective photodynamic agent that can be useful in the treatment of tumors with depths in the range of 1 cm.

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“…Chlorin e 6 belongs to the chlorin compounds, which contain a number of photophysical properties superior to those of HPD and photofrin 2. It possesses a higher photodynamic activity under in vitro and in vivo conditions (17–19). In contrast to photofrin, chlorin e 6 absorption peak is in the long‐wave spectral area (660 nm), and this results in improved light penetrability in biological tissues.…”

Section: Discussionmentioning

confidence: 99%

“…Chlorin e 6 was synthesized at the Laboratory of Photochemistry, National Academy of Sciences of the Republic of Belarus (16). PDT techniques were developed in experiments on animals with inoculated tumors and necrobiotic changes were demonstrated to occur in the tumor tissue exposed to PDT with chlorin e 6 , followed by subsequent regression of the transplanted tumors in rats (17–19).…”

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confidence: 99%