Sensitivity of Field Strains of<i>Gibberella Fujikuroi (Fusarium</i>Section<i>Liseola</i>) to Benomyl and Hygromycin B

Yan, Keying; Dickman, Martin B.; Xu, Jin‐Rong; Leslie, John F.

doi:10.1080/00275514.1992.12026269

Cited by 18 publications

(3 citation statements)

References 21 publications

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“…GUS reporter gene expression was assessed only for the F. verticillioides isolates recovered on MMϩHyg, and all were positive. The apparent isolation of only the transformed strain RRC PATgus on MMϩHyg, which contains 150 g of hygromycin B per ml, is consistent with the native level of hygromycin sensitivity among field isolates of F. verticillioides (56).…”

Section: Survey Ofmentioning

confidence: 66%

Detoxification of Corn Antimicrobial Compounds as the Basis for Isolating Fusarium verticillioides and Some Other Fusarium Species from Corn

Glenn

Hinton

Yates

et al. 2001

Appl Environ Microbiol

115

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The preformed antimicrobial compounds produced by maize, 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3-one and its desmethoxy derivative 2,4-dihydroxy-2H-1,4-benzoxazin-3-one, are highly reactive benzoxazinoids that quickly degrade to the antimicrobials 6-methoxy-2-benzoxazolinone (MBOA) and 2-benzoxazolinone (BOA), respectively. Fusarium verticillioides ‫؍(‬ F. moniliforme) is highly tolerant to MBOA and BOA and can actively transform these compounds to nontoxic metabolites. Eleven of 29 Fusarium species had some level of tolerance to MBOA and BOA; the most tolerant, in decreasing order, were F. verticillioides, F. subglutinans, F. cerealis ‫؍(‬ F. crookwellense), and F. graminearum. The difference in tolerance among species was due to their ability to detoxify the antimicrobials. The limited number of species having tolerance suggested the potential utility of these compounds as biologically active agents for inclusion within a semiselective isolation medium. By replacing the pentachloronitrobenzene in Nash-Snyder medium with 1.0 mg of BOA per ml, we developed a medium that resulted in superior frequencies of isolation of F. verticillioides from corn while effectively suppressing competing fungi. Since the BOA medium provided consistent, quantitative results with reduced in vitro and taxonomic efforts, it should prove useful for surveys of F. verticillioides infection in field samples.

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Section: Survey Ofmentioning

confidence: 66%

Detoxification of Corn Antimicrobial Compounds as the Basis for Isolating Fusarium verticillioides and Some Other Fusarium Species from Corn

Glenn

Hinton

Yates

et al. 2001

Appl Environ Microbiol

115

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“…We tested the multiplex PCR amplification on 102 strains from mating populations A to F (24) and 60 strains from mating population H (4) and found that the amplification products detected were the same as those predicted based on the results of sexual crosses. The amplified MAT DNA fragments cosegregated with mating type in a genetic mapping cross (9,24). Thus, the amplified fragments map with 95% certainty to a 2.3-map unit region that includes MAT and are unlikely to map more than one map unit from MAT, if they are not coincident with it.…”

Section: Analysis Of Mat-2mentioning

confidence: 99%

“…We examined 60 strains from G. fujikuroi mating population H (4) and 80 strains from G. fujikuroi mating populations A to F (24). To demonstrate Mendelian segregation and cosegregation of the molecular markers with their corresponding mating phenotype, we examined 128 progeny of a cross between two F. verticillioides isolates from the A mating population (23).…”

mentioning

confidence: 99%

PCR-Based Identification of MAT-1 and MAT-2 in the Gibberella fujikuroi Species Complex

Steenkamp

Wingfield

Coutinho

et al. 2000

Appl Environ Microbiol

Self Cite

155

106

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All sexually fertile strains in the Gibberella fujikuroi species complex are heterothallic, with individual mating types conferred by the broadly conserved ascomycete idiomorphs MAT-1 and MAT-2. We sequenced both alleles from all eight mating populations, developed a multiplex PCR technique to distinguish these idiomorphs, and tested it with representative strains from all eight biological species and 22 additional species or phylogenetic lineages from this species complex. In most cases, either an ϳ800-bp fragment from MAT-2 or an ϳ200-bp fragment from MAT-1 is amplified. The amplified fragments cosegregate with mating type, as defined by sexual cross-fertility, in a cross of Fusarium moniliforme (Fusarium verticillioides). Neither of the primer pairs amplify fragments from Fusarium species such as Fusarium graminearum, Fusarium pseudograminearum, and Fusarium culmorum, which have, or are expected to have, Gibberella sexual stages but are thought to be relatively distant from the species in the G. fujikuroi species complex. Our results suggest that MAT allele sequences are useful indicators of phylogenetic relatedness in these and other Fusarium species.Fusarium isolates in the Gibberella fujikuroi species complex include important fungal pathogens of agricultural crops and trees and may be divided into at least eight different biological species and 32 additional asexual species or phylogenetic lineages (4, 10, 13, 15, 16). All sexually fertile species in the G. fujikuroi species complex are dimictic, i.e., two isolates are cross-fertile if they carry the different mating-type idiomorphs MAT-1 and MAT-2 (4, 6, 9, 13), which share no sequence similarity with respect to either DNA sequence or the proteins encoded (5). The MAT-2 idiomorphs thus far characterized have a conserved HMG (high-mobility-group) domain (3,5,6,9). The MAT-1 idiomorphs have a conserved ␣-domain (5, 25), but no MAT-1 alleles have yet been described from any members of the G. fujikuroi species complex.Our objectives in this study were (i) to sequence conserved portions of the MAT-1 and MAT-2 alleles from the eight known G. fujikuroi mating populations, (ii) to develop a multiplex PCR reaction to be used for the identification of both mating-type idiomorphs within the defined biological species of the G. fujikuroi species complex, (iii) to determine the range of Fusarium species which have Gibberella teleomorphs to which this technique can be successfully applied, and (iv) to test the use of the sequence of the MAT idiomorphs for phylogenetic analyses. This technique eases the identification of strains to be used in crosses to identify new biological species and eliminates the need for sexual crosses to score this trait. MAT sequence variations may be used as phylogenetic characters, providing another marker that can be used to test phylogenies for robustness.

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Characterization of Fusarium verticillioides strains by PCR‐RFLP analysis of the intergenic spacer region of the rDNA

et al. 2005

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Thirty-three Fusarium verticillioides strains from diverse origins and hosts have been analysed for fumonisin production and characterized in order (i) to detect the variability present in this species and (ii) to discriminate among isolates. The method used was a combination of polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) generated by restriction endonucleases applied to the IGS region (intergenic spacer of rDNA). All the F. verticillioides strains associated with crops produced fumonisins B 1 and B 2 except those isolated from banana. Analysis of the IGS region by PCR-RFLP proved to be useful to detect variability within F. verticillioides and allowed discrimination of two related groups of isolates belonging to distinct lineages differing in fumonisin production and host preferences: the fumonisin-producing group associated with cereals and the fumonisin non-producing group associated with banana. The method used facilitates early detection and characterization of F. verticillioides strains required to control both types of pathogens and to evaluate plant exposure to the toxin, quality of the raw material to be processed and the potential fumonisin contamination in order to prevent fumonisins entering the food chain.

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Sensitivity of Field Strains ofGibberella Fujikuroi (FusariumSectionLiseola) to Benomyl and Hygromycin B

Cited by 18 publications

References 21 publications

Detoxification of Corn Antimicrobial Compounds as the Basis for Isolating Fusarium verticillioides and Some Other Fusarium Species from Corn

Detoxification of Corn Antimicrobial Compounds as the Basis for Isolating Fusarium verticillioides and Some Other Fusarium Species from Corn

PCR-Based Identification of MAT-1 and MAT-2 in the Gibberella fujikuroi Species Complex

Characterization of Fusarium verticillioides strains by PCR‐RFLP analysis of the intergenic spacer region of the rDNA

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