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A ␤-tubulin gene from a UV-irradiated benomyl-resistant mutant of Fusarium moniliforme was isolated, cloned, and sequenced. The gene encodes a 446-amino-acid polypeptide with homology to other fungal ␤-tubulins. RNA blot analysis showed expression of the gene during vegetative growth and conidial germination but no expression during conidiation. A point mutation, which likely confers benomyl resistance, has been identified in the cloned gene; this mutation results in a single amino acid substitution of asparagine for tyrosine at position 50. Expression of benomyl resistance in the mutant was also cold sensitive. Sexual crosses between the mutant and a wild-type strain indicated cosegregation of benomyl resistance and cold sensitivity.

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SIGNIFICANCE:Although the high prevalence of digital eyestrain has been well established, to date, there is no objective measurement of this condition. Previous studies have suggested that digital eyestrain may be associated with decreased critical fusion frequency, but the published evidence to support this association is sparse.PURPOSE: This study sought to determine whether symptoms of digital eyestrain after a sustained computer task are indeed associated with changes in critical fusion frequency. METHODS:The experiment was performed on 30 young visual-normal subjects. They attended two sessions, during which they undertook a 20-minute reading task. This comprised either reading random words from a tablet computer or a story from a printed children's book. Critical fusion frequency was measured both before and immediately after each of the reading tasks. In addition, the level of digital eyestrain was assessed by subjects completing a questionnaire regarding ocular and visual symptoms experienced during each of the reading trials. RESULTS:The mean increase in digital eyestrain symptoms after the digital and printed conditions was 11.37 (standard error of the mean [SEM], 2.23) and 4.40 (SEM, 1.34), respectively. Both the post-task symptom change ( P < .001) and the difference between the two reading conditions ( P = .004) were significant. The mean change in critical fusion frequency after the digital and printed conditions was −0.42 (SEM, 0.25) and −0.72 (SEM, 0.26), respectively. Neither the post-task change nor the difference between the two reading conditions was significant. In addition, when considering the digital condition only, no significant correlation was observed between the changes in critical fusion frequency and reported symptoms.CONCLUSIONS: These results do not support the proposal that changes in critical fusion frequency can be used as an objective measure of eyestrain or other symptoms of digital eyestrain.

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