Sensitivity of porcine peripheral blood leukocytes to gamma irradiation in vivo, in vitro and ex vivo

Zárybnická, Lenka; Šinkorová, Zuzana; Sinkora, J; Blaha, V.; Vávrová, Jiřina; Pejchal, Jaroslav; Österreicher, Jan

doi:10.3109/09553002.2011.548437

Cited by 8 publications

(3 citation statements)

References 26 publications

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“…However, there might be several problems with this approach. Lymphocyte numbers significantly decrease after irradiation of pigs in vivo or of pig blood in vitro in a dose-dependent manner, but reduction of lymphocyte numbers after irradiation in vivo proceeds much faster than after irradiation in vitro [21]. Furthermore, a significantly different response to irradiation is achieved in vivo than in vitro or ex vivo .…”

Section: Discussionmentioning

confidence: 99%

Attempted Depletion of Passenger Leukocytes by Irradiation in Pigs

Tai

Zhu

Lin

et al. 2011

Journal of Transplantation

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Allograft/xenograft rejection is associated with “passenger leukocyte” migration from the organ into recipient lymph nodes. In Study 1, we attempted to deplete leukocytes from potential kidney “donor” pigs, using two regimens of total body irradiation. A dose of 700 cGy was administered, followed by either 800 cGy (“low-dose”) or 1,300 cGy (“high dose”) with the kidneys shielded. Neither regimen was entirely successful in depleting all leukocytes, although remaining T and 8 cell numbers were negligible. Study 2 was aimed at providing an indication of whether near-complete depletion of leukocytes had any major impact on kidney allograft survival. In non-immunosuppressed recipient pigs, survival of a kidney from a donor that received high-dose irradiation was compared with that of a kidney taken from a non-irradiated donor. Kidney graft survival was 9 and 7 days, respectively, suggesting that depletion had little impact on graft survival. The lack of effect may have been related to (i) inadequate depletion of passenger leukocytes, thus not preventing a direct T cell response, (ii) the presence of dead or dying leukocytes (antigens), thus not preventing an indirect T cell response, or (iii) constitutive expression of MHC class II and B7 molecules on the porcine vascular endothelium, activating recipient T cells.

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Section: Discussionmentioning

confidence: 99%

Attempted Depletion of Passenger Leukocytes by Irradiation in Pigs

Tai

Zhu

Lin

et al. 2011

Journal of Transplantation

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“…The porcine model is similar to the human model in some aspects related to irradiation, such as phylogeny, immune system and immune response (argument of the similarity between humans and animals) (68). The results of the in vitro model cannot be extrapolated to the in vivo model with respect to the absolute leukocyte population, because of scavenging mechanisms and resident leukocytes only present in the in vivo model (argument about tradition and scientific relevance) (68).…”

Section: Arguments For Animal Experimentationmentioning

confidence: 99%

Animal Experimentation in Oncology and Radiobiology: Arguments for and Against Following a Critical Literature Review

Girard

Bertrand-Grenier

Drolet

2022

Canadian Journal of Bioethics

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“…Peripheral blood (PB) comprises plasma and circulating cells; leukocytes are a particularly radiosensitive blood component (5,6). The cytogenetic effects of 131 I on PB lymphocytes have been analyzed (7)(8)(9)(10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

Predictive factors of cytotoxic damage in radioactive iodine treatment of differentiated thyroid cancer patients

Monzen

Mariya

Wójcik

et al. 2015

Molecular and Clinical Oncology

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Radioactive iodine (I) therapy in patients suffering from differentiated thyroid cancer (DTC) is a targeted treatment commonly used for thyroid ablation and locoregional and distant metastatic spread management. Despite a significant proportion of the I dose entering the circulation, there is currently no detailed information regarding its effect on the blood cell system. In order to assess the cytotoxic effects ofI therapy on the circulatory system, blood cell levels, thyroid-related hormones and CD45 cell cytotoxicity were estimated in blood collected from patients with DTC. The micronuclei (MN) frequency of the peripheral blood CD45 cell fraction was significantly increased after 30 days of I therapy compared to that prior to treatment, although a strong individual variation was observed. A significantly negative correlation between MN frequency and the level of platelets and plateletcrit was observed; however, there was no such correlation with thyroid-related hormones. These resultS suggest that the correlation between MN frequency and the platelet system may serve as a biomarker of exposure and, possibly, of sensitivity in DTC patients undergoingI therapy following thyroid and lymph node surgery.

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Sensitivity of porcine peripheral blood leukocytes to gamma irradiation in vivo, in vitro and ex vivo

Abstract: All three tested experimental arrangements demonstrated the radiosensitivity of lymphocytes and the radioresistance of peripheral blood granulocytes. These in vivo and in vitro approaches, as well as the newly introduced ex vivo observations, appear to be relevant to biodosimetry.

Cited by 8 publications

References 26 publications

Attempted Depletion of Passenger Leukocytes by Irradiation in Pigs

Attempted Depletion of Passenger Leukocytes by Irradiation in Pigs

Animal Experimentation in Oncology and Radiobiology: Arguments for and Against Following a Critical Literature Review

Predictive factors of cytotoxic damage in radioactive iodine treatment of differentiated thyroid cancer patients

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