Sensitivity of Pure Cultures of Bradyrhizobium Japonicum  to Fungicides

Kukol, K.P.; Vorobey, N. A.; Kots, S.Yа.

doi:10.35868/1997-3004.30.20-31

Cited by 5 publications

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“…We previously conducted studies to determine the resistance of several strains of nodule bacteria B. japonicum to plant protection agents using the "agar diffusion method". Both strains PC07 and B78 in pure culture (in vitro) were found to be relatively insensitive to the fungicides Fever and Maxim XL [24,25].…”

Section: Resultsmentioning

confidence: 99%

“…With the use of 2 production norms of the fungicide, the inhibition zone around the "wells" for strain PC07 was 7.9 mm, and for strain B78, it was 9.0 mm. Therefore, the strains were identified as having low sensitivity to Maxim XL [24,25].…”

Section: Discussionmentioning

confidence: 99%

“…The use of recombinant strains resistant to fungicides in pure culture as model objects is a promising direction in order to identify the possibility of the combined or sequential use of recombinant strains of rhizobia and PPP to increase the productivity of leguminous crops. In pure culture, recombinant strains show a higher level of resistance to a number of fungicides, including Fever and Maxim XL compared to the original parental forms and a number of other investigated strains [24,25].…”

Section: Methodsmentioning

confidence: 96%

“…The consumption rate of the preparations for the treatment of soybean seeds is 0.2-0.4 l/t. The use of the working solution is 10 l/t of seeds [24].…”

Section: Methodsmentioning

confidence: 99%

“…In last years, Fever and Maxim XL are widely used among soybean fungicides. They are less toxic to nodule bacteria [24,25]. At the same time to "List of pesticides and agrochemicals approved for use in Ukraine in 2023" includes many of preparations that are used to treat soybean seeds in order to limit the development of phytopathogens and contain the same active substances as Fever and Maxim XL.…”

Section: Introductionmentioning

confidence: 99%

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Viability of Nodule Bacteria Bradyrhizobium japonicummon Soybean Seeds by Treatment With Fungicides During Extended Storage

Vorobey,

Kukol,

Kots

et al. 2023

Innov Biosyst Bioeng

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Background. With the increase in soybean cultivation areas, inoculants are becoming increasingly sought after. They are not only compatible with the original preparations for seed treatment of soybean but also offer the possibility of applying them for several days or even months before sowing into the soil. Objective. The viability of new strains of nodule bacteria Bradyrhizobium japonicum (strain PC07 and strain B78) was investigated on the surface of soybean seeds treated with fungicides Fever and Maxim XL during the extended storage of inoculated seeds. Additionally, their ability for nitrogen fixation under symbiotic conditions was evaluated. Methods. Сultivation, serial dilution method, determination of bacterial titer of inoculants, quantification of colony forming units (CFU), gas chromatography. Results. In laboratory conditions, on the soybean seeds of Almaz and Vasylkivska varieties, the viability of the nodule bacteria B. japonicum PC07 and B78, which are more resistant to the active substances of these fungicides in pure culture, decreased. This reduction was dependent on the storage period, the fungicide used, and the strain of inoculant. One day after inoculation on the seeds without the use of fungicide, 68.6–75.4% CFU of the initial number was retained. Seeds treated with the fungicide Fever contained 36.5–38.3% CFU, and those treated with Maxim XL contained 26.2–27.0% CFU. The use of inoculants based on fungicide-resistant strains of B. japonicum PC07 and B78 also provided high cell viability – 2.41–2.8×106 (on the seeds without treatment), 1.40–1.70×106 (with Fever) and 0.8–1.17×106 (with Maxim XL) CFU/seed after 5 days of storage. The nitrogen-fixing activity of root nodules in soybean plants of Almaz and Vasylkivska varieties, whose seeds were treated with Fever, decreased on both varieties by 18.4–22.4% and 32.1–39.5%, respectively and for treatment with Maxim XL, the reduction was 24.5–33.7% and 47.7–75.2%, respectively, during the storage of seeds for 5 and 7 days before sowing, compared to control variants (seeds without fungicide treatment). Conclusions. The utilization of fungicide-resistant strains of B. japonicum PC07 and B78 for bacterization of soybean seeds treated with fungicides Fever and Maxim XL provides a high inoculation titer during 5 days of storage. Viability of microbial cells on seeds treated with fungicides significantly diminishes during storage for more than 7–14 days. Insufficient titer of CFU of B. japonicum strains PC07 and B78 on the seed surface after 14 days of storage can considerably impede the efficacy of biopreparations. Therefore, it is crucial to seek substances that can enhance the resistance of rhizobia on seeds to the adverse effects of fungicide seed treatment, ensuring a longer period of their viability.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 96%

“…The consumption rate of the preparations for the treatment of soybean seeds is 0.2-0.4 l/t. The use of the working solution is 10 l/t of seeds [24].…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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