Sensitivity of Seeds to Chemical Mutagens, Detection of DNA Polymorphisms and Agro-Metrical Traits in M1 Generation of Coffee (Coffea arabica L.)

Vargas-Segura, César; López-Gamboa, Emmanuel; Araya-Valverde, Emanuel; Valdez-Melara, Marta; Gatica-Arias, Andrés

doi:10.1007/s12892-019-0175-0

Cited by 11 publications

(4 citation statements)

References 40 publications

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“…Various investigations have documented a negative relationship between seed germination and concentrations of EMS [6,23,[32][33][34]. Therefore, it is recommended to perform a dose-response analysis to determine the average lethal dose for the study model in particular.…”

Section: Morphology and Physiological Processes In Fruits And Seedsmentioning

confidence: 99%

“…On the other hand, Vargas-Segura et al [34] they mention that the mean lethal dose of EMS, for treatments with C. arabica seeds is between 160 mM to 240 mM for eight hours, in this range is where there are variations in terms of the percentage of germination and emergence, as in the length of the aerial part and the root; at lower concentrations it does not generate visible phenotypic changes and high doses generated embryo death.…”

Section: Seedsmentioning

confidence: 99%

“…These changes are attributed to the effect of EMS on the F0 seeds, which affected the embryo. Changes in the F2 generation appeared during the seedling stage in chlorophyll formation, causing slow growth in all phenological stages [34].…”

Section: Evaluation Of Mutation With Ems In Later Generationsmentioning

confidence: 99%

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Ethyl Methanesulfonate as Inductor of Somaclonal Variants in Different Crops

Joya-Dávila¹,

Gutiérrez-Miceli²

2020

Phyton

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Ethyl methanesulfonate is a chemical mutagen, which is currently being used in plant breeding, to increase genetic variability in genes of agronomic interest, of species useful in agriculture. It primarily causes single base point mutations by inducing guanine alkylation, resulting in GC to AT transitions. Its effect is different between clones of a genotype and between genotypes of the same species. This review presents the results obtained in recent research, where its effect on plant tissues, callus, and cells in suspension has been evaluated. Changes in the phenotypic expression of somaclonal variants were reported, involving morphology, production of secondary metabolites, changes in metabolic routes of resistance, tolerance to stress, increased seed yield, among others. In addition, this review compiles the doses and guidelines to consider before using this mutagen, which can serve as a guide for future trials in deciding the response variables, the type of plant explants and the selection of the study model. Mutant lines have allowed plant breeders to have a collection of plants with different characteristics, in places where the cultivar does not have its center of origin. It is important to note that it is still necessary to continue evaluating the heritability of mutations and their behaviour in the environment where they will be established, in order to obtain new varieties of plants that can be cultivated with uniformity in their genetic response.

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Section: Morphology and Physiological Processes In Fruits And Seedsmentioning

confidence: 99%

Section: Seedsmentioning

confidence: 99%

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Ethyl Methanesulfonate as Inductor of Somaclonal Variants in Different Crops

Joya-Dávila¹,

Gutiérrez-Miceli²

2020

Phyton

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“…It is a handy strategy proved to achieve rapid genetic changes that bring about additional variability in the qualitative and quantitative traits and supplement existing germplasm for cultivar improvement in breeding programs [ [14] , [15] , [16] , [17] ]. It was successfully employed for creating additional heritable variability in many crops including maize [ 18 ], rice [ 15 ], sesame [ 14 , 19 ], coffee [ 20 ], tomato [ 21 ] and brassica [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Treatment of seeds with sodium azide for quantitative and qualitative capsule traits at M2 generation of Fourteen Ethiopian sesame (Sesamum indicum L.) genotypes

Weldemichael

Baryatsion

Sbhatu

et al. 2023

Heliyon

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Evaluation of Coffee (Coffea arabica L. var. Catuaí) Tolerance to Leaf Rust (Hemileia vastatrix) Using Inoculation of Leaf Discs Under Controlled Conditions

Rojas-Chacón,

Echeverría-Beirute,

Gatica-Arias

2023

Mutation Breeding in Coffee With Special Reference to Leaf Rust

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Coffee leaf rust (CLR), caused by the obligate biotrophic fungus Hemileia vastatrix, is considered one of the most devastating diseases of Arabica coffee. The use of leaf rust resistant or tolerant coffee varieties is a critical component for effective management of this disease at the farm level. Conventional breeding of Arabica coffee for leaf rust resistance requires many years of breeding and field-testing. Induced mutagenesis is an effective tool to increase genetic variability and generate new alleles with potential benefit for addressing abiotic and biotic stresses such as leaf rust in Arabica coffee. Efficient screening methods are required to evaluate coffee germplasm or mutant populations for resistance to H. vastatrix. Here, we present a screening method that uses inoculation of leaf discs in a controlled environment. The method was evaluated using M1V1 and M2 plants derived from chemically mutagenized Arabica coffee cell suspensions. In this method, the first rust symptoms appear on the leaf discs approximately 29 days after inoculation while the disease severity and incidence can be scored about 47 days after inoculation. Our results show that the methodology is simple, efficient and suitable to rapidly screen large mutant populations in a small area.

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Sensitivity of Seeds to Chemical Mutagens, Detection of DNA Polymorphisms and Agro-Metrical Traits in M1 Generation of Coffee (Coffea arabica L.)

Cited by 11 publications

References 40 publications

Ethyl Methanesulfonate as Inductor of Somaclonal Variants in Different Crops

Ethyl Methanesulfonate as Inductor of Somaclonal Variants in Different Crops

Treatment of seeds with sodium azide for quantitative and qualitative capsule traits at M2 generation of Fourteen Ethiopian sesame (Sesamum indicum L.) genotypes

Evaluation of Coffee (Coffea arabica L. var. Catuaí) Tolerance to Leaf Rust (Hemileia vastatrix) Using Inoculation of Leaf Discs Under Controlled Conditions

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