Sensitivity programmable ratiometric electrochemical aptasensor based on signal engineering for the detection of aflatoxin B1 in peanut

Li, Yuye; Liu, Dong; Zhu, Chengxi; Shen, Xiuli; Yang, Liu; You, Tianyan

doi:10.1016/j.jhazmat.2019.122001

Cited by 100 publications

(41 citation statements)

References 37 publications

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“…This result indicated that the dual-signal change of the ratio sensor can effectively improve the sensitivity of the uorescence aptasensor, and the homogeneous detection method improved the stability of the sensor. ELISA 0.24-2.21 0.13 [20] Liquid Chromatography 0.05-2 0.108 [21] Electrochemistry 0.05-20 0.016 [22] Fluorescence 0.1-0.8 0.07 [23] Fluorescence 0.05-100 0.05 [24] Fluorescence 0.015-15 0.003 [25] Fluorescence 0.05-100 0.0126 This work…”

Section: Detection Of Afb1mentioning

confidence: 94%

A Ratiometric Fluorescent Aptamer Homogeneous Biosensor Based on Hairpin Structure Aptamer for AFB1 Detection

Feng

Zhao

Wei

et al. 2022

Preprint

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On the basis of aptamer (Apt) with hairpin structure and fluorescence resonance energy transfer (FRET), a ratio fluorescent aptamer homogeneous sensor was prepared for the determination of Aflatoxin B1 (AFB1). Initially, the Apt labeled simultaneously with Cy5, BHQ2, and cDNA labeled with Cy3 were formed a double-stranded DNA through complementary base pairing. The fluorescent aptamer sensor demonstrates a weak fluorescence emission of Cy3 and a high fluorescence emission of Cy5 due to the quenching effect of BHQ2. The double-stranded DNA structure will be disintegrated in the presence of AFB1, resulting the removal of Cy3 and the close of Cy5 with BHQ2. The fluorescence signal of Cy3 and Cy5 were restored and quenched respectively. Thus, the ratio change of FCy3 to FCy5 was used to realized the detection of AFB1 with wider detection range and lower limit of detection (LOD). The response of the optimized protocol for AFB1 detection was wider linear range from 0.05 ng/mL to 100 ng/mL and the LOD was 12.6 pg/mL. The sensor designed in this strategy has the advantages of simple preparation and fast signal response. It has been used for the detection of AFB1 in labeled corn and wine, indicating it had good application potential in practical samples.

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Section: Detection Of Afb1mentioning

confidence: 94%

A Ratiometric Fluorescent Aptamer Homogeneous Biosensor Based on Hairpin Structure Aptamer for AFB1 Detection

Feng

Zhao

Wei

et al. 2022

Preprint

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“…Ferrocene-labelled aptamer (Fc-apt) served as the response signal and reduced graphene oxide (THI-rGO) functionalized with thionine was used as the reference signal. In this study, ratiometric detection was achieved by formation of Fc-apt-AFB1 complex which resulted in decreased current intensity of Fc (I Fc ), and increased current intensity of THI (I THI ) [ 123 ]. It is worth mentioning that sensitivity of this sensor can be adjusted by changing the assembly of Fc-apt, which exhibited high sensitivity and selectivity.…”

Section: Rapid Detection Strategiesmentioning

confidence: 99%

Recent advances in immunoassays and biosensors for mycotoxins detection in feedstuffs and foods

Yang

Wang

et al. 2021

J Animal Sci Biotechnol

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Mycotoxins are secondary metabolites produced by fungus. Many mycotoxin species are highly toxic and are frequently found in cereals and feedstuffs. So, powerful detection methods are vital and effective ways to prevent feed contamination. Traditional detection methods can no longer meet the needs of massive, real-time, simple, and fast mycotoxin monitoring. Rapid detection methods based on advanced material and sensor technology are the future trend. In this review, we highlight recent progress of mycotoxin rapid detection strategies in feedstuffs and foods, especially for simultaneous multiplex mycotoxin determination. Immunoassays, biosensors, and the prominent roles of nanomaterials are introduced. The principles of different types of recognition and signal transduction are explained, and the merits and pitfalls of these methods are compared. Furthermore, limitations and challenges of existing rapid sensing strategies and perspectives of future research are discussed.

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“…Enlightened by this idea, Li et al. (2020) fabricated a sensitivity‐adjustable ratiometric EC aptasensor for AFB 1 detection based on the Thi functionalized reduced GO (Thi‐rGO) nanocomposite, AuNPs and Fc‐labeled aptamer (Fc‐apt; Figure 1f). Thi‐rGO was employed not noly as an EC substrate, but also as a signal producer.…”

Section: Typical Aptasensors For Mycotoxin Detectionmentioning

confidence: 99%

“…(e) Schematic illustration of a hemin/G‐quadruplex/DTN/AuNRs/NH 2 ‐AuE based EC aptasensor for OTA detection (Wei & Zhang, 2018b). (f) Schematic illustration of a ratiometric EC aptasensor based on Thi–rGO nanocomposite, AuNPs and Fc‐apt (Li et al., 2020). (g) Schematic illustration of the dual‐target EC aptasensor based on rMoS 2 –AuNPs for multi‐mycotoxin detection (Han et al., 2020)…”

Section: Typical Aptasensors For Mycotoxin Detectionmentioning

confidence: 99%

Aptasensors for mycotoxins in foods: Recent advances and future trends

Hou

Jia

Sheng

et al. 2021

Comp Rev Food Sci Food Safe

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Mycotoxin contamination in foods has posed serious threat to public health and raised worldwide concern. The development of simple, rapid, facile, and cost‐effective methods for mycotoxin detection is of urgent need. Aptamer‐based sensors, abbreviated as aptasensors, with excellent recognition capacity to a wide variety of mycotoxins have attracted ever‐increasing interest of researchers because of their simple fabrication, rapid response, high sensitivity, low cost, and easy adaptability for in situ measurement. The past few decades have witnessed the rapid advances of aptasensors for mycotoxin detection in foods. Therefore, this review first summarizes the reported aptamer sequences specific for mycotoxins. Then, the recent 5‐year advancements in various newly developed aptasensors, which, according to the signal output mode, are divided into electrochemical, optical and photoelectrochemical categories, for mycotoxin detection are comprehensively discussed. A special attention is taken on their strengths and limitations in real‐world application. Finally, the current challenges and future perspectives for developing novel highly reliable aptasensors for mycotoxin detection are highlighted, which is expected to provide powerful references for their thorough research and extended applications. Owing to their unique advantages, aptasensors display a fascinating prospect in food field for safety inspection and risk assessment.

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Sensitivity programmable ratiometric electrochemical aptasensor based on signal engineering for the detection of aflatoxin B1 in peanut

Cited by 100 publications

References 37 publications

A Ratiometric Fluorescent Aptamer Homogeneous Biosensor Based on Hairpin Structure Aptamer for AFB1 Detection

A Ratiometric Fluorescent Aptamer Homogeneous Biosensor Based on Hairpin Structure Aptamer for AFB1 Detection

Recent advances in immunoassays and biosensors for mycotoxins detection in feedstuffs and foods

Aptasensors for mycotoxins in foods: Recent advances and future trends

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