Sensitivity to electrical stimulation of human immunodeficiency virus type 1 and MAGIC-5 cells

Kumagai, Etsuko; Tominaga, Masato; Harada, Shinji

doi:10.1186/2191-0855-1-23

Cited by 11 publications

(9 citation statements)

References 28 publications

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“…Additionally, we emphasized that the codified number of ions produced from LRD are responsible to reduce the SARS-CoV-2 viral load but not the precipitate produced by metal rods used in the LRD. This data is consistent with previously published data about the effect of electric stimulation on viral infectivity [15,25,26]. The mechanism of how electric stimulation reduces the infectivity of respiratory viruses is still unclear.…”

Section: Discussionsupporting

confidence: 92%

“…Interestingly, stimulation of auricular vagus nerve by electric frequencies reduced the side effects of lung inflammations caused by SARS-CoV-2 [25]. Additionally, patients infected with human immunodeficiency virus type 1 (HIV-1) exposed to low electric potential frequencies showed minimal side effects and reduction of the viral infectivity [16,26]. Interestingly, the previous reports are consistent with our hypothesis about viral infectivity reduction using the codified number of ions produced by LRD.…”

Section: Discussionsupporting

confidence: 89%

“…Despite the fact that rapid progress in our understanding of the structural mechanisms of virus entry promises new discoveries, approaches capable of 'outwitting' the virus, no entry clinically used inhibitors or treatment protocols have far been developed on the basis of forecasts by structural models, and the primary source of new information inhibitors are still discovered by screening large libraries of small molecules. The papered LRD system in this study depends on the production of the codified number of ions that can exterminate the biological materials of the treated viruses [15,25,26]. Interestingly, stimulation of auricular vagus nerve by electric frequencies reduced the side effects of lung inflammations caused by SARS-CoV-2 [25].…”

Section: Discussionmentioning

confidence: 97%

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Using Safe Calculated Low Power of Electrons to Cut, Analyze and Exterminate the Outer and Inner Biological Elements of SARS-CoV-2, MERS-CoV-2 and Influenza viruses in Vitro

Madboly¹,

Shehata

Nashed

et al. 2022

JSRR

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Background: The treatment of pneumonia viruses with antiviral drugs produced mild to severe side effects until the immune system worked against the invaded virus. Objective: Therefore, it is crucial to develop an efficient alternative therapy with lower side effects against infection of pneumonia viruses. Methods: We hereby designated a novel device termed a a Life Restoration Device (LRD to produce electric frequencies with lower potential. The electric frequencies cancan specifically destroy the nucleic acid materials and viral envelope rather than the plasma of the infected cell. Using a glass tube model the infected cells with propagated viruses were filled in the glass tube and two nickel-coated copper rods were inserted into both ends. Then the two rods were connected into the LRD. Lower potential electric frequencies were applied for 30 min and 60 min onto the infected cells. Results: The treatment of the infected cell culture of MERS-CoV and SARS-CoV-2 using LRD for 30 min significantly reduced the viral infectivity to 83% and 22% respectively. After 60 min of MERS-CoV and SARS-CoV-2 exposure to LRD, the infectivity reduced to 21% and 1% respectively. Additionally, based on the data of transmission electron microscopy of the H5N1 virus and electrophoretic patterns of different types of viruses, the nucleic acid materials of the treated viruses were reduced based on the non-treated viruses. The electric frequencies sensitivity produced by LRD can reduce the fluidity of the viral envelope rather than the plasma membrane of the infected cells. Conclusion: Treatment of pneumonia viruses with electric stimulation is a new alternative therapy but needs more investigations. The data obtained from this study is important to develop an effective alternative viral therapy.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 97%

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Using Safe Calculated Low Power of Electrons to Cut, Analyze and Exterminate the Outer and Inner Biological Elements of SARS-CoV-2, MERS-CoV-2 and Influenza viruses in Vitro

Madboly¹,

Shehata

Nashed

et al. 2022

JSRR

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“…Treating the coronavirus by an electric stimulus may sound absurd, however literature provides interesting findings and indicates that electric stimulation may be an effective approach to reduce viral burden in COVID-19 patients. Furthermore, the application of electric current is known to improve drug penetration [9] . A short impulse of low voltage current can irreversibly damage the viral membrane.…”

Section: Justification Of Hypothesismentioning

confidence: 99%

Potential of electric stimulation for the management of COVID-19

Allawadhi¹,

Khurana

Allwadhi

et al. 2020

Medical Hypotheses

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The COVID-19 pandemic is the most devastating health emergency that humans have seen over the past century. The war against the disease has been handicapped by unavailability of effective therapeutic options. Till date, there is no clinically approved vaccine or drug for the treatment of COVID-19, and the ongoing search to find a novel therapy is progressing at pandemic pace. Herein, we propose a novel hypothesis based on sound research evidence that electric stimulation can be a potential adjuvant to the currently used symptomatic therapies and antiviral drugs. Based on preclinical evidence, we propose that electric stimulation can improve respiratory functions, inhibit SARS-CoV-2 growth, reduce pain, boost immunity and improve the penetration of antiviral drugs. We envisage that our hypothesis, if used clinically as an adjuvant, may significantly improve the therapeutic outcomes of the current treatment regimen being used around the globe for the management of COVID-19.

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“…Several studies [ 17 , 18 , 19 ] indicated that cells, bacteria, and viruses are affected by a pulsed electric current or direct current (DC) with different wavefronts. Kumagai et al [ 20 , 21 ] used a constant DC potential of 1.0 V to stimulate HIV type 1 (HIV-1) and MAGIC-5 cells. Their results indicated that HIV-1LAI and HIV-1KMT infections were inhibited by 90% following 3 min of stimulation; however, the healthy cells were not damaged.…”

Section: Introductionmentioning

confidence: 99%

Reduction of Viral and Bacterial Activity by Using a Self-Powered Variable-Frequency Electrical Stimulation Device

et al. 2023

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Viruses and bacteria, which can rapidly spread through droplets and saliva, can have serious effects on people’s health. Viral activity is traditionally inhibited using chemical substances, such as alcohol or bleach, or physical methods, such as thermal energy or ultraviolet-light irradiation. However, such methods cannot be used in many applications because they have certain disadvantages, such as causing eye or skin injuries. Therefore, in the present study, the electrical stimulation method is used to stimulate a virus, namely, coronavirus 229E, and two types of bacteria, namely, Escherichia coli and Staphylococcus aureus, to efficiently reduce their infectivity of healthy cells (such as the Vero E6 cell in a viral activity-inhibition experiment). The infectivity effects of the aforementioned virus and bacteria were examined under varying values of different electrical stimulation parameters, such as the stimulation current, frequency, and total stimulation time. The experimental results indicate that the activity of coronavirus 229E is considerably inhibited through direct-current pulse stimulation with a current of 25 mA and a frequency of 2 or 20 Hz. In addition, E. coli activity was reduced by nearly 80% in 10 s through alternating-current pulse stimulation with a current of 50 mA and a frequency of 25 Hz. Moreover, a self-powered electrical stimulation device was constructed in this study. This device consists of a solar panel and battery to generate small currents with variable frequencies, which has advantages of self-powered and variable frequencies, and the device can be utilized on desks, chairs, or elevator buttons for the inhibition of viral and bacterial activities.

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Sensitivity to electrical stimulation of human immunodeficiency virus type 1 and MAGIC-5 cells

Cited by 11 publications

References 28 publications

Using Safe Calculated Low Power of Electrons to Cut, Analyze and Exterminate the Outer and Inner Biological Elements of SARS-CoV-2, MERS-CoV-2 and Influenza viruses in Vitro

Using Safe Calculated Low Power of Electrons to Cut, Analyze and Exterminate the Outer and Inner Biological Elements of SARS-CoV-2, MERS-CoV-2 and Influenza viruses in Vitro

Potential of electric stimulation for the management of COVID-19

Reduction of Viral and Bacterial Activity by Using a Self-Powered Variable-Frequency Electrical Stimulation Device

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