Sensitivity to oxazolone induced dermatitis is transferable with gut microbiota in mice

Abstract

Atopic Dermatitis (AD) has been associated with gut microbiota (GM) dysbiosis in humans, indicating a causative role of GM in AD etiology. Furthermore, the GM strongly correlates to essential disease parameters in the well-known oxazolone-induced mouse model of AD. Here, we demonstrate that it is possible to transfer both a high-responding and a low-responding AD phenotype with GM from conventional mice to germ-free mice. The mice inoculated with the high-responding GM had significantly higher clinical score, increased ear thickness, and increased levels of IL-1β, TNFα, IL-4, IL-5, and IL-6 compared to the mice inoculated with the low-responding GM. The inter-individual variation was in general not affected by this increase in effect size. Germ-free mice induced with AD revealed a high disease response as well as high inter-individual variation indicating protective properties of certain microbial taxa in this model. This study underlines that the GM has a strong impact on AD in mouse models, and that the power of studies may be increased by the application of mice inoculated with a specific GM from high responders to increase the effect size.

The gut microbiome alterations in allergic and inflammatory skin diseases – an update

“…One of the favourable mechanism is promoting Th 1 response in atopic patients . The role of microbiota has been also observed in oxazolone‐induced mouse model of atopic dermatitis, where atopic dermatitis phenotype correlated with the gut microbiota transfer . Potential beneficial role of probiotic supplementation may also modulate the inflammatory processes in the course of psoriasis by the suppression of interleukin 23 and Th 17 axis .…”

“…29 The role of microbiota has been also observed in oxazolone-induced mouse model of atopic dermatitis, where atopic dermatitis phenotype correlated with the gut microbiota transfer. 36 Potential beneficial role of probiotic supplementation may also modulate the inflammatory processes in the course of psoriasis by the suppression of interleukin 23 and Th 17 axis. 37 The peri-neonatal antibiotic therapy in imiquimod-induced psoriasis resulted in gut dysbiosis and skin lesion aggravation.…”

Investigating the role of Interleukin-33 and Soluble ST2 in pediatric atopic dermatitis

“…According to J. Penders et al study, intestinal microbiota plays an important role in the development of AD in early childhood [37]. An experimental murine model demonstrates that germ-free mice fail to develop oral tolerance, presence of gut microbiota have a protective role against AD formation [38,39]. According to our study, stool testing did not reveal any activation of IL-33/sST2 axis in AD patients, further testing is needed.…”

Traiter la dermatite atopique par les probiotiques

“…La composition complexe de la flore intestinale, mise en évidence par des études de séquençage, rend la balance entre équilibre du microbiote intestinal et dysbiose intestinale d'autant plus instable [2]. Or, une dysbiose du microbiote intestinal chez l'homme pourrait être à l'origine de nombreuses pathologies associées ou non à l'intestin (maladies intestinales chroniques, diabète, obésité, certains cancers ou certaines maladies allergiques dont la DA) [2,4]. Chez les patients atteints de DA, une dysbiose du microbiote intestinal est associée à une diversité réduite de la population bactérienne commensale intestinale [4].…”