Sensitization of bacteria to danofloxacin by temperate prophages

Froshauer, S; Silvia, Annette M.; Chidambaram, Monjula; Sharma, Brij; Weinstock, George M.

doi:10.1128/aac.40.6.1561

Cited by 14 publications

(19 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Basically, the las system regulates the two others (Latifi et al, 1996; Pesci et al, 1997), and while rhl inhibits pqs, pqs is able to activate rhlI . At the same time pqs regulates eDNA release, membrane vesicles production and prophage induction (Figure 1; Froshauer et al, 1996; Mashburn and Whiteley, 2005; Allesen-Holm et al, 2006). …”

Section: Edna Production In Cultured Microorganismsmentioning

confidence: 99%

“…Specifically, it depends on the las and pqs systems (Renelli et al, 2004; Nakamura et al, 2008). The pqs system is not only implicated in eDNA production, as quinolones are well-known for triggering prophage induction and cause cell lysis (Froshauer et al, 1996). Considering that the motility is necessary for phages propagation, Allesen-Holm et al (2006), analyzed a motility mutant and concluded that the PQS mediates prophage induction and the flagella/pili-dependent phage propagation are involved in eDNA release.…”

Section: Edna Production In Cultured Microorganismsmentioning

confidence: 99%

See 1 more Smart Citation

Mechanisms and Regulation of Extracellular DNA Release and Its Biological Roles in Microbial Communities

2017

View full text Add to dashboard Cite

The capacity to release genetic material into the extracellular medium has been reported in cultures of numerous species of bacteria, archaea, and fungi, and also in the context of multicellular microbial communities such as biofilms. Moreover, extracellular DNA (eDNA) of microbial origin is widespread in natural aquatic and terrestrial environments. Different specific mechanisms are involved in eDNA release, such as autolysis and active secretion, as well as through its association with membrane vesicles. It is noteworthy that in microorganisms, in which DNA release has been studied in detail, the production of eDNA is coordinated by the population when it reaches a certain cell density, and is induced in a subpopulation in response to the accumulation of quorum sensing signals. Interestingly, in several bacteria there is also a relationship between eDNA release and the development of natural competence (the ability to take up DNA from the environment), which is also controlled by quorum sensing. Then, what is the biological function of eDNA? A common biological role has not been proposed, since different functions have been reported depending on the microorganism. However, it seems to be important in biofilm formation, can be used as a nutrient source, and could be involved in DNA damage repair and gene transfer. This review covers several aspects of eDNA research: (i) its occurrence and distribution in natural environments, (ii) the mechanisms and regulation of its release in cultured microorganisms, and (iii) its biological roles. In addition, we propose that eDNA release could be considered a social behavior, based on its quorum sensing-dependent regulation and on the described functions of eDNA in the context of microbial communities.

show abstract

Section: Edna Production In Cultured Microorganismsmentioning

confidence: 99%

Section: Edna Production In Cultured Microorganismsmentioning

confidence: 99%

Mechanisms and Regulation of Extracellular DNA Release and Its Biological Roles in Microbial Communities

2017

View full text Add to dashboard Cite

show abstract

“…Previous studies demonstrated that the presence of prophages increased the sensitivity of M. haemolytica to danofloxacin, and exposure to this antimicrobial induced prophage production (Froshauer et al . ). We selected 66 M. haemolytica isolates with high and low levels of enrofloxacin sensitivity based on disc diffusion assays and found no correlation between presence of inducible phages and the sensitivity of M. haemolytica to this antibiotic.…”

Section: Discussionmentioning

confidence: 97%

“…Both danofloxacin and enrofloxacin inhibit bacterial DNA gyrase (Froshauer et al . ), resulting in DNA damage during replication. Many bacteria possess an SOS‐response system which is an inducible DNA repair system that allows bacteria to overcome DNA damage (Foster ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Investigation ofMannheimia haemolyticabacteriophages relative to host diversity

et al. 2013

View full text Add to dashboard Cite

Aims: This study aimed to characterize the impact of lytic and temperate bacteriophages on the genetic and phenotypic diversity of Mannheimia haemolytica from feedlot cattle. Methods and Results: Strictly lytic phages were not detected from bovine nasopharyngeal (n = 689) or water trough (n = 30) samples, but Myoviridaeor Siphoviridae-like phages were induced from 54 of 72 M. haemolytica strains by mitomycin C, occasionally from the same strain. Phages with similar restriction fragment length polymorphism profiles (RFLP ! 70% relatedness) shared common host serotypes 1 or 2 (P < 0Á0001). Likewise, phages with similar RFLP tended to occur in genetically related host bacteria (70-79% similarity). Host range assays showed that seven phages from host serotypes 1, 2 and 6 lysed representative strains of serotypes 1, 2 or 8. The genome of vB_MhM_1152AP from serotype 6 was found to be collinear with P2-like phage φMhaA1-PHL101. Conclusions: Prophages are a significant component of the genome of M. haemolytica and contribute significantly to host diversity. Further characterization of the role of prophage in virulence and persistence of M. haemolytica in cattle could provide insight into approaches to control this potential respiratory pathogen. Significance and Impact of the Study: This study demonstrated that prophages are widespread within the genome of M. haemolytica isolates and emphasized the challenge of isolating lytic phage as a therapeutic against this pathogen.

show abstract

Extracellular DNA as Matrix Component in Microbial Biofilms

Chiang

Tolker‐Nielsen

2010

Nucleic Acids and Molecular Biology

View full text Add to dashboard Cite

Sensitization of bacteria to danofloxacin by temperate prophages

Cited by 14 publications

References 8 publications

Mechanisms and Regulation of Extracellular DNA Release and Its Biological Roles in Microbial Communities

Mechanisms and Regulation of Extracellular DNA Release and Its Biological Roles in Microbial Communities

Investigation ofMannheimia haemolyticabacteriophages relative to host diversity

Extracellular DNA as Matrix Component in Microbial Biofilms

Contact Info

Product

Resources

About