Sensivity and specifity of ELISA test for diagnosis of brucellosis

Vakili, Zarichehr; Heravi, Mansooreh Momen; Sharif, Alireza

doi:10.1016/j.ijid.2010.02.403

Cited by 2 publications

(3 citation statements)

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“…Diagnostic methods of brucellosis are usually based on detecting specific antibodies in the patient's serum. Nowadays many serological tests are applied for the diagnosis of human brucellosis (3,4), Most routine tests are serum agglutination tests based on Wright and 2-Mercaptoethanol (2-ME), which were developed in 1897 by Weight et al (5) and nowadays they are still used as reference tests in clinical laboratories. Other tests, which were developed later, include Rose Bengal, complement fixation, indirect coombs and the Enzyme Linked Immunosorbent Assay (ELISA) (6).…”

Section: Introductionmentioning

confidence: 99%

Epidemiology and the Agreement Rate of Serological Tests in Human Brucellosis in North East of Iran

Sadeghian

Saedi

Sadeghian

et al. 2015

Arch Clin Infect Dis

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Background: Brucellosis still remains a major health problem with different symptoms and various diagnostic methods. Diagnostic methods of brucellosis are usually based on detecting specific antibodies in the patient's serum. Nowadays, many serological tests are applied for the diagnosis of human brucellosis. Most routine tests are serum agglutination tests based on Wright and 2-Mercaptoethanol (2-ME). Objectives: The aim of this study (cross sectional study) was to evaluate the prevalence of brucellosis and assess the degree of agreement among serum samples of suspected brucellosis serological tests routinely performed in Mashhad, Iran. Patients and Methods:This study was conducted in Mashhad from August 2011 to September 2012. Sera (2 -3 mL) were collected from 83 cases suspected of brucellosis among 594 patients. Ten serum samples were collected from healthy subjects as control sera. Rose Bengal test for initial screening and Wright and 2 ME as standard tests were conducted to determine antibody titers. Thereafter, IgG and IgM levels were determined by the Enzyme Linked Immunosorbent Assay (ELISA) method. Results: Among 83 serum samples, Rose Bengal test was able to identify 20 (12%) positive specimens; the standard tube agglutination test was able to detect 30 (18%) positive samples, and the ELISA IgG and ELISA IgM were able to trace 42 (21%) and 13 (6.5%) positive samples, respectively. Ten control samples had negative results for the ELISA method. The results were calculated by the Kappa formula. The highest level of agreement was among 1 = KRB-SAT tests and the lowest level of agreement was among tests K ELISA IgM-IgG = 0.30. Conclusions: According to the results, brucellosis has remained endemic in this region. Most cases were detected by ELISA IgG. The highest kappa agreements were between tests KRB-SAT, K RB-IgG and K SAT-IgG , while the lowest levels of agreement were between tests SATIgM and ELISA IgM-IgG. Considering that ELISA IgM results are covered by SAT and ELISA IgG test results, applications of this test do not seem necessary.

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Section: Introductionmentioning

confidence: 99%

Epidemiology and the Agreement Rate of Serological Tests in Human Brucellosis in North East of Iran

Sadeghian

Saedi

Sadeghian

et al. 2015

Arch Clin Infect Dis

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“…A study by Mohraz et al, 9 the sensitivity and specificity of IgG ELISA were 93% and 100%, respectively. The sensitivity of IgG ELISA in the study by Peeridogaheh et al 10 was 75.4% which is less as compared to the studies done by Mohrazet al, 9 (93%) and Vakili et al ( 93.7%) 11 . However, the sensitivity of IgM ELISA in the study by Peeridogaheh et al 10 was higher that is 40.6% than that in Vakili et al, 11 (12.5%) study.…”

Section: Discussionmentioning

confidence: 67%

“…The sensitivity of IgG ELISA in the study by Peeridogaheh et al 10 was 75.4% which is less as compared to the studies done by Mohrazet al, 9 (93%) and Vakili et al ( 93.7%) 11 . However, the sensitivity of IgM ELISA in the study by Peeridogaheh et al 10 was higher that is 40.6% than that in Vakili et al, 11 (12.5%) study. The specificity of IgG ELISA of Mohrazet al, 9 study (100%) and IgM ELISA of Vakiliet al, 11 study (100%) support the result of Peeridogaheh et al 10 It is observed that from the review of literature, that it is difficult to compare seroprevalence of brucellosis in different studies as it varies from place to place and time to time.…”

Section: Discussionmentioning

confidence: 67%

Seroprevalence of Brucella Agglutinins in Patients with Pyrexia of Unknown Origin Attending a Tertiary Care Rural Hospital

Damke¹,

Damke²

2020

jemds

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Sensivity and specifity of ELISA test for diagnosis of brucellosis

Abstract: e35214th International Congress on Infectious Diseases (ICID) Abstracts -Analysis of H. pylori virulence gene vacAs1 and cagA by conventional PCR method, emphasizing high sensitivity of PCR method.

Cited by 2 publications

References 0 publications

Epidemiology and the Agreement Rate of Serological Tests in Human Brucellosis in North East of Iran

Epidemiology and the Agreement Rate of Serological Tests in Human Brucellosis in North East of Iran

Seroprevalence of Brucella Agglutinins in Patients with Pyrexia of Unknown Origin Attending a Tertiary Care Rural Hospital

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