Sensor impedimétrico para la detección de bacterias patogénicas mediante péptidos antimicrobianos

Hoyos‐Nogués, Mireia; Brosel-Oliu, S.; Abramova, Natalia A.; Bratov, Andrey; Mas-Moruno, C.; Gil, Francisco José Soler

doi:10.5821/sibb.24.1.5195

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“…The authors report that the biosensor obtained is capable of selectively detecting Salmonella spp cells with a detection limit of 15 CFU/mL. On the other hand, Hoyos, et al reported the design of an impedimetric sensor based on antimicrobial peptides for the early detection of periodontopathogenic bacteria [21]. The system is able to detect S. sanguinis in 1 h with LOD of 10 CFU/mL in KCl and 100 CFU/mL in artificial saliva.…”

Section: Evaluation Of the Biosensor Towards Detection Of E Coli 241 Evaluation Of The Linear Response Range Of The Biosensormentioning

confidence: 99%

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A Bioinspired Peptide in TIR Protein as Recognition Molecule on Electrochemical Biosensors for the Detection of E. coli O157:H7 in an Aqueous Matrix

et al. 2021

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Currently, the detection of pathogens such as Escherichia coli through instrumental alternatives with fast response and excellent sensitivity and selectivity are being studied. Biosensors are systems consisting of nanomaterials and biomolecules that exhibit remarkable properties such as simplicity, portable, affordable, user‑friendly, and deliverable to end‑users. For this, in this work we report for the first time, to our knowledge, the bioinformatic design of a new peptide based on TIR protein, a receptor of Intimin membrane protein which is characteristic of E. coli. This peptide (named PEPTIR‑1.0) was used as recognition element in a biosensor based on AuNPs‑modified screen‑printed electrodes for the detection of E. coli. The morphological and electrochemical characteristics of the biosensor obtained were studied. Results show that the biosensor can detect the bacteria with limits of detection and quantification of 2 and 6 CFU/mL, respectively. Moreover, the selectivity of the system is statistically significant towards the detection of the pathogen in the presence of other microorganisms such as P. aeruginosa and S. aureus. This makes this new PEPTIR‑1.0 based biosensor can be used in the rapid, sensitive, and selective detection of E. coli in aqueous matrices.

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Section: Evaluation Of the Biosensor Towards Detection Of E Coli 241 Evaluation Of The Linear Response Range Of The Biosensormentioning

confidence: 99%

“…Recently, peptide-based biosensors have been widely studied in pathogen detection and disease diagnosis due to their sensitivity, low cost, and specificity [18][19][20][21][22]. These devices are probably the most versatile systems due to the physicochemical properties of the peptides.…”

Section: Introductionmentioning

confidence: 99%