Sensor System for Simultaneous Measurement of Oxygen and Hydrogen Peroxide Concentration During Glucose Oxidase Activity

Abstract

An amperometric sensor system, based on a repetitive double step potential method at a glassy carbon electrode, has been developed for the simultaneous measurement of hydrogen peroxide and oxygen concentrations. The current measured at a potential of À1 V (vs. AgyAgClysaturated Cl À ) corresponds to the sum of the reduction currents of hydrogen peroxide and dissolved oxygen. The current measured at À0.55 V (vs. AgyAgClysaturated Cl À ) is due to the reduction of dissolved oxygen to hydrogen peroxide. Alternatively, the concentration of dissolved oxygen can also be determined using a Clark electrode. The concentration of hydrogen peroxide and dissolved oxygen during enzymatic conversion of glucose can be followed on line and be used to control the process.

Evidence for [18-Crown-6 Na]2[S2O4] in Methanol and Dissociation to Na2S2O4and 18-Crown-6 in the Solid State; Accounting for the Scarcity of Simple Oxy Dianion Salts of Alkali Metal Crown Ethers in the Solid State

“…Sodium dithionite is an important industrial chemical used as a reductive bleaching agent for pulp and paper, and for the reduction of Vat dyes and sulfur containing dyes . Sodium dithionite is also used widely in research as a reducing agent in organic, organometallic, and inorganic chemistry.…”

Miniaturized implantable pressure and oxygen sensors based on polydimethylsiloxane thin films

“…The limit of the current plateau is given by Levich equation [12], which is controlled by the diffusion rate of the dissolved oxygen in solution at this potential. For best performance, electrochemical oxygen sensors usually operate at the potential where the plateau current occurs [13] – [15]. Thus, in our experiments, a steady dc bias of -1.0 V was applied to the Ag/AgCl reference electrode of the sensor.…”

Measurement of glucose concentrations in human plasma using a glucose biosensor

“…The enzymeimmobilized membrane was subsequently positioned on the surface of a dissolved oxygen sensor. Since many research groups have already used Clark-type electrodes to detect the change of oxygen concentration during enzymatic conversion of glucose [14,15], in this article we report a cheaper and improved biosensor which is developed from a traditional Clark-type oxygen electrode to detect the glucose content in human blood plasma.The detection scheme was based on the enzymatic reactions of glucose leading to the depletion in the dissolved oxygen level in the base solution and then the decrease in the oxygen level was monitored and related to the glucose concentration. The proposed glucose biosensor has the promising features of good stability, low cost, simple design, and ease of operation.…”

“…The enzymeimmobilized membrane was subsequently positioned on the surface of a dissolved oxygen sensor. Since many research groups have already used Clark-type electrodes to detect the change of oxygen concentration during enzymatic conversion of glucose [14,15], in this article we report a cheaper and improved biosensor which is developed from a traditional Clark-type oxygen electrode to detect the glucose content in human blood plasma.…”