Sensory neurons in the human jugular ganglion

Atsumi, Keiichiro; Yajima, Takahiro; Tachiya, Daisuke; Kokubun, Souichi; Shoji, Nobuyuki; Sasano, Takashi; Ichikawa, Hiroyuki; Sato, Tadasu

doi:10.1016/j.tice.2020.101344

Cited by 13 publications

(6 citation statements)

References 45 publications

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“…Murine VG are composed of jugular and nodose ganglion fused together into a single complex, while these ganglia are anatomically separated in humans (1,4,52). The human nodose ganglion alone is considerably larger than the mouse vagal ganglion including a proportionally higher number of neuronal cell bodies (2,53,54). However, we see a similar distinct TMC3 mRNA expression pattern in sections generated from human donor VG (Fig.…”

Section: Discussionmentioning

confidence: 57%

Lung-innervating neurons expressingTmc3can induce broncho-constriction and dilation with direct consequences for the respiratory cycle

Kortmann,

Huang,

Tsai

et al. 2023

Preprint

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Sensory neurons of the vagal ganglia (VG) innervate lungs and play a critical role in maintaining airway homeostasis. However, the specific VG neurons that innervate lungs, and the mechanisms by which these neurons sense and respond to airway insults, are not well understood. Here, we identify a subpopulation of lung-innervating VG neurons defined by their expression of Tmc3. Single cell transcriptomics illuminated several subpopulations of Tmc3+ sensory neurons, revealing distinct Piezo2- and Trpv1-expressing subclusters. Furthermore, Tmc3 deficiency in VG neurons leads to global and subcluster specific transcriptional changes related to metabolic and ion channel function. Importantly, we show that broncho-constriction and dilation can be modulated through inhibition or activation of Tmc3+ VG neurons resulting in a decrease or increase of end-expiratory lung volume, respectively. Together, our data show that Tmc3 is a marker of lung-innervating neurons and may play a pivotal role in maintaining fundamental inspiratory and expiratory processes.

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Section: Discussionmentioning

confidence: 57%

Lung-innervating neurons expressingTmc3can induce broncho-constriction and dilation with direct consequences for the respiratory cycle

Kortmann,

Huang,

Tsai

et al. 2023

Preprint

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“…Data about the cerebral falx, other cerebral portions, and cerebellar dura mater were obtained from 1, 3, and 14 digital images (each image size = 660 µm × 820 µm), respectively, in each section. The proportion of their length to the area of each region was calculated for immunoreactive density 51 – 53 . The average of such immunoreactive density was recorded for each animal.…”

Section: Methodsmentioning

confidence: 99%

Distribution and possible function of galanin about headache and immune system in the rat dura mater

Shimazaki

Yajima

Ichikawa

et al. 2022

Sci Rep

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Galanin (GAL) is a nociceptive transmitter or modulator in the trigeminal sensory system. In this study, GAL expression was investigated in the rat dura mater to demonstrate its possible function in headache using immunohistochemical techniques. The cerebral falx and cerebellar dura mater received abundant blood and nerve supply, and were significantly thicker compared to other portions in the cerebral dura mater. GAL-immunoreactivity was expressed by cell and nerve fiber profiles. Presumed macrophages and dendritic cells contained GAL-immunoreactivity, and co-expressed with CD11b-immunoreactivity. Many isolated and perivascular nerve fibers also showed GAL-immunoreactivity. In addition, GAL-immunoreactive nerve fibers were present in the vicinity of macrophages and dendritic cells with either GAL- or ED1-immunoreactivity. GAL-immunoreactive cells and nerve fibers were common in the cerebral falx and cerebellar dura mater and infrequent in other portions. And, GAL-immunoreactive nerve fibers usually co-expressed calcitonin gene-related peptide (CGRP)-immunoreactivity. In the trigeminal ganglion, a substantial proportion of sensory neurons innervating the dura mater contained GAL-immunoreactivity (mean ± SD, 3.4 ± 2.2%), and co-expressed CGRP-immunoreactivity (2.7 ± 2.1%). The present study may suggest that GAL is associated with nociceptive transduction or modulation in the dura mater. GAL also possibly plays a role in the immune mechanism of the dura mater.

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“…Following nickel ammonium sulfate‐intensified diaminobenzidine reaction, they were dehydrated in a graded series of alcohols, cleared in lemosol and cover‐slipped with Softmount (Wako Pure Chemical Industries, Ltd., Japan) (Liu et al, 2020; Suzuki et al, 2012). The specificity of primary antibodies has been described elsewhere (Atsumi et al, 2020; Kawashima et al, 2021).…”

Section: Methodsmentioning

confidence: 99%

Distribution of neuronal cells which contain dopamine β‐hydroxylase, tyrosine hydroxylase, neuropeptide Y and vasoactive intestinal polypeptide in the human internal carotid nerve

Sato

Yajima

Kokubun

et al. 2021

The Anatomical Record

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The human internal carotid nerve (ICN) occasionally has a swelling beneath the external opening of the carotid canal. In this study, the presence and distribution of neuronal cells were investigated in the bilateral ICNs of nine human cadavers. Among 44.4% of the cadavers, swellings were detected in the ICN. Their diameters ranged from 1.7 to 3.6 mm (average ± SD = 2.6 ± 0.7 mm). Thirty‐eight percent of these swellings were large (diameter > 3 mm) and showed an oval shape. The large swelling contained many neuronal cells. However, the ICNs with or without a swelling <3 mm diameter were mostly free from neuronal cells (93.3%). Only in one human cadaver, the right ICN without a swelling had a small number of neuronal cells. By the present immunohistochemical method, ICN neurons contained catecholamine‐synthesizing enzymes and neuropeptides. Dopamine‐beta hydroxylase‐ and tyrosine hydroxylase‐immunoreactivity were mostly expressed by ICN neurons. More than half of them also contained neuropeptide Y‐immunoreactivity. However, vasoactive intestinal polypeptide‐immunoreactive ICN neurons were relatively infrequent. Substance P‐ and calcitonin gene‐related peptide‐immunoreactive ICN neurons could not be detected. By the cell size analysis, neuropeptide Y‐immunoreactive neurons were significantly smaller than neuropeptide Y‐immunonegative neurons in the ICN. The present study suggests that ICN neurons have a sympathetic function in the human.

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Sensory neurons in the human jugular ganglion

Cited by 13 publications

References 45 publications

Lung-innervating neurons expressingTmc3can induce broncho-constriction and dilation with direct consequences for the respiratory cycle

Lung-innervating neurons expressingTmc3can induce broncho-constriction and dilation with direct consequences for the respiratory cycle

Distribution and possible function of galanin about headache and immune system in the rat dura mater

Distribution of neuronal cells which contain dopamine β‐hydroxylase, tyrosine hydroxylase, neuropeptide Y and vasoactive intestinal polypeptide in the human internal carotid nerve

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