Sentinel Lymph Node Characterization with a Dual-Targeted Molecular Ultrasound Contrast Agent

Nam, Kibo; Stanczak, Maria; Forsberg, Flemming; Liu, Ji‐Bin; Eisenbrey, John R.; Solomides, Charalambos; Lyshchik, Andrej

doi:10.1007/s11307-017-1109-3

Cited by 18 publications

(36 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Two‐step molecular lymphosonography was performed in all animals according to a previously described protocol . At first, SLNs were detected by using the Sonazoid US contrast agent injected subdermally around the tumor .…”

Section: Methodsmentioning

confidence: 99%

“…The center frequencies varied over 6 to 8 MHz depending on the depth of interest. For CEUS imaging, the built‐in Cadence Pulse Sequencing technique was used . Imaging clips (50–100 frames) were acquired from the SLNs before and after applying a high–mechanical index (>0.8) destruction pulse sequence .…”

Section: Methodsmentioning

confidence: 99%

“…There have been studies to detect and characterize SLNs noninvasively . A study by Akers et al showed the feasibility of detecting regional SLNs of rats in vivo with combined fluorescence and photoacoustic imaging.…”

mentioning

confidence: 99%

“…Contrast‐enhanced ultrasound (CEUS) imaging has also been used to detect and characterize SLNs . Shimazu et al achieved 98% accuracy in SLN detection using CEUS with Sonazoid (GE Healthcare, Oslo, Norway) compared to a blue dye and/or radiocolloid method in 100 patients with breast cancer.…”

mentioning

confidence: 99%

“…The detection of metastasis in SLNs was based on nonspecific accumulation of the contrast agent quantitatively as well as qualitatively, and somewhat limited accuracy of 60% to 80% was achieved . To improve accuracy in characterization of SLNs, as reported in a previous study, we introduced molecular lymphosonography using targeted contrast agents . In that study, a total of 25 melanoma SLNs from 9 swine were first identified by Sonazoid, and then, after SLN identification, metastatic involvement was detected by using intravenous injection of a dual‐targeted contrast agent .…”

mentioning

confidence: 99%

See 4 more Smart Citations

Performance of Molecular Lymphosonography for Detection and Quantification of Metastatic Involvement in Sentinel Lymph Nodes

Nam

Stapp

Liu

et al. 2018

J of Ultrasound Medicine

Self Cite

View full text Add to dashboard Cite

Objectives To assess the performance of molecular lymphosonography with dual‐targeted microbubbles in detecting and quantifying the metastatic involvement in sentinel lymph nodes (SLNs) using a swine melanoma model. Methods Targeted microbubbles were labeled with P‐selectin and αVβ3‐integrin antibodies. Control microbubbles were labeled with immunoglobulin G antibodies. First lymphosonography with Sonazoid (GE Healthcare, Oslo, Norway) was used to identify SLNs. Then dual‐targeted and control microbubbles were injected intravenously to detect and quantify metastatic disease in the SLNs. Distant non‐SLNs were imaged as benign controls. All evaluated lymph nodes (LNs) were surgically removed, and metastatic involvement was characterized by a histopathologic analysis. Two radiologists blinded to histopathologic results assessed the baseline B‐mode images of LNs, and the results were compared to the histologic reference standard. The mean intensities of targeted and control microbubbles within the examined LNs were measured and compared to the LN histologic results. Results Thirty‐five SLNs and 34 non‐SLNs from 13 Sinclair swine were included in this study. Twenty‐one SLNs (62%) were malignant, whereas 100% of non‐SLNs were benign. The sensitivity of B‐mode imaging for metastatic LN diagnosis for both readers was relatively high (90% and 71%), but the specificity was very poor (50% and 58%). The sensitivity and specificity of molecular lymphosonography for metastatic LN detection were 91% and 67%, respectively. The mean intensities from dual‐targeted microbubbles correlated well with the degree of metastatic LN involvement (r = 0.6; P < 0.001). Conclusions Molecular lymphosonography can increase the specificity of metastatic LN detection and provide a measure to quantify the degree of metastatic involvement.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Performance of Molecular Lymphosonography for Detection and Quantification of Metastatic Involvement in Sentinel Lymph Nodes

Nam

Stapp

Liu

et al. 2018

J of Ultrasound Medicine

Self Cite

View full text Add to dashboard Cite

show abstract

50‐nm Gas‐Filled Protein Nanostructures to Enable the Access of Lymphatic Cells by Ultrasound Technologies

Shen,

Li,

Wang

et al. 2024

Advanced Materials

View full text Add to dashboard Cite

Ultrasound imaging and ultrasound‐mediated gene and drug delivery are rapidly advancing diagnostic and therapeutic methods; however, their use is often limited by the need of microbubbles, which cannot transverse many biological barriers due to their large size. Here we introduce 50‐nm gas‐filled protein nanostructures derived from genetically engineered gas vesicles that we referred to as 50nmGVs. These diamond‐shaped nanostructures have hydrodynamic diameters smaller than commercially available 50‐nm gold nanoparticles and are, to our knowledge, the smallest stable, free‐floating bubbles made to date. 50nmGVs can be produced in bacteria, purified through centrifugation, and remain stable for months. Interstitially injected 50nmGVs can extravasate into lymphatic tissues and gain access to critical immune cell populations, and electron microscopy images of lymph node tissues reveal their subcellular location in antigen‐presenting cells adjacent to lymphocytes. We anticipate that 50nmGVs can substantially broaden the range of cells accessible to current ultrasound technologies and may generate applications beyond biomedicine as ultrasmall stable gas‐filled nanomaterials.This article is protected by copyright. All rights reserved

show abstract

Bridging Smart Nanosystems with Clinically Relevant Models and Advanced Imaging for Precision Drug Delivery

Zhou,

Liu,

Wang

et al. 2024

Advanced Science

View full text Add to dashboard Cite

Intracellular delivery of nano‐drug‐carriers (NDC) to specific cells, diseased regions, or solid tumors has entered the era of precision medicine that requires systematic knowledge of nano‐biological interactions from multidisciplinary perspectives. To this end, this review first provides an overview of membrane‐disruption methods such as electroporation, sonoporation, photoporation, microfluidic delivery, and microinjection with the merits of high‐throughput and enhanced efficiency for in vitro NDC delivery. The impact of NDC characteristics including particle size, shape, charge, hydrophobicity, and elasticity on cellular uptake are elaborated and several types of NDC systems aiming for hierarchical targeting and delivery in vivo are reviewed. Emerging in vitro or ex vivo human/animal‐derived pathophysiological models are further explored and highly recommended for use in NDC studies since they might mimic in vivo delivery features and fill the translational gaps from animals to humans. The exploration of modern microscopy techniques for precise nanoparticle (NP) tracking at the cellular, organ, and organismal levels informs the tailored development of NDCs for in vivo application and clinical translation. Overall, the review integrates the latest insights into smart nanosystem engineering, physiological models, imaging‐based validation tools, all directed towards enhancing the precise and efficient intracellular delivery of NDCs.

show abstract

Sentinel Lymph Node Characterization with a Dual-Targeted Molecular Ultrasound Contrast Agent

Abstract: The results indicate that dual-targeted microbubbles labeled with P-selectin and αβ-integrin antibodies may aid in detecting metastatic involvement in SLNs of melanoma.

Cited by 18 publications

References 55 publications

Performance of Molecular Lymphosonography for Detection and Quantification of Metastatic Involvement in Sentinel Lymph Nodes

Performance of Molecular Lymphosonography for Detection and Quantification of Metastatic Involvement in Sentinel Lymph Nodes

50‐nm Gas‐Filled Protein Nanostructures to Enable the Access of Lymphatic Cells by Ultrasound Technologies

Bridging Smart Nanosystems with Clinically Relevant Models and Advanced Imaging for Precision Drug Delivery

Contact Info

Product

Resources

About