Seonamhaeicola maritimus sp. nov., isolated from coastal sediment

Abstract

A Gram-stain-negative, facultatively anaerobic, non-motile, rod-shaped and orange-pigmented bacterium, designated 1505T, was isolated from marine sediment that was obtained off the coast of Weihai, PR China. Strain 1505T was found to grow at 10–35 °C (optimum, 28 °C), at pH 6.0–9.0 (optimum, 7.5) and in the presence of 1–4 % (w/v) NaCl (optimum, 2 %). Cells were positive for oxidase and catalase activity. The 16S rRNA gene based phylogenetic analysis revealed that the nearest phylogenetic neighbours of strain 1505T were Seonamhaeicola algicola Gy8T (97.1 %), Seonamhaeicola marinus B011T (96.3 %) and Seonamhaeicola aphaedonensis KCTC 32578T (95.6 %). Based on phylogenomic analysis, the average nucleotide identity values between strain 1505T and S. algicola Gy8T, S. marinus B011T and S. aphaedonensis KCTC 32578T were 75.9, 76.0 and 77.7 %, respectively; the digital DNA–DNA hybridization values based on the draft genomes between strain 1505T and S. algicola Gy8T, S. marinus B011T and S. aphaedonensis KCTC 32578T were 20.0, 20.7 and 21.4 %, respectively. Menaquinone-6 (MK-6) was detected as the major respiratory quinone. The dominant cellular fatty acids were iso-C15 : 1 G and C18 : 1ω9c. The DNA G+C content of strain 1505T was 33.3 mol%. The polar lipids included phosphatidylethanolamine, six aminolipids and four unidentified lipids. Based on its phylogenetic and phenotypic characteristics, strain 1505T is considered to represent a novel species of the genus Seonamhaeicola , for which the name Seonamhaeicola maritimus sp. nov. is proposed. The type strain is 1505T (=KCTC 72528T=MCCC 1H00389T).

Ornithinimicrobium laminariae sp. nov., isolated from the kelp Laminaria japonica

“…The 16S rRNA gene was amplified by PCR amplification using forward primer 27F and reverse primer 1492R [23]. On the basis of the almost-complete 16S rRNA gene sequence, a blast search (www.ncbi.nlm.nih.gov) and the EzBiocloud (www.ezbiocloud.net) were used to identify the phylogenetic relationships and calculate the pairwise 16S rRNA gene sequence similarity [24].…”

“…The 16S rRNA gene was amplified by PCR amplification using forward primer 27F and reverse primer 1492R [23]. On the basis of the almost-complete 16S rRNA gene sequence, a blast search (www.ncbi.nlm.nih.gov) and the EzBiocloud (www.ezbiocloud.…”

Shewanella nanhaiensis sp. nov., a marine bacterium isolated from sediment of South China Sea, and emended descriptions of Shewanella woodyi, Shewanella hanedai and Shewanella canadensis

“…The 16S rRNA gene was amplified by the universal primers 27F and 1492R as previously described [21]. The PCR product was purified and ligated into the PMD19-T vector (TaKaRa) and cloned according to the manufacturer′s instructions.…”

Salinimicrobium nanhaiense sp. nov. and Salinimicrobium oceani sp. nov., two novel species of the family Flavobacteriaceae isolated from the South China Sea

“…The 16S rRNA gene was amplified by the universal primers 27F and 1492R as described previously [21]. The almost full-length 16S rRNA gene sequences of strain J15B81-2 T (1511 bp) and J15B91 T (1510 bp) were determined and confirmed with the draft genome sequence.…”

“…Moreover, the ANIb and dDDH values between strain J15B81-2 T and J15B91 T were 81.1-85.8 % and 31.5 %, respectively. The 16S rRNA gene was amplified by the universal primers 27F and 1492R as described previously [21]. The almost full-length 16S rRNA gene sequences of strain J15B81-2 T (1511 bp) and J15B91 T (1510 bp) were determined and Table 1.…”