Separate cytotoxic T lymphocyte subsets recognize the different H-2 specificities.

Abstract

Using a monolayer adsorption technique, the fine specificity of cytotoxic effector T lymphocytes (CTL) generated against allogeneic or semi-allogeneic H-2 haplotypes was investigated. The results show that: (a) CTL reacting with the private specificity expressed on an H-2.K molecule can be separated from those reacting with the public specificities expressed on the same molecule and (b) the CTL that recognize cross-reacting H-2 determinants (public specificities) can also be separated into several subpopulations. These data support the hypothesis that an allogeneic stimulation induces a large number of independent T cell clones that react with H-2 determinants.

Role of self and foreign antigenic determinants in allogeneic and self-restricted cytotoxic T cell recognition.

“…3D and E) confirmed this hypothesis, thus demonstrating that the CTL clones that mediated lysis against TNP-self targets were not responsible for the lysis of FITC-self targets. Thus, alloantigen-activated CTL cultures appear to be composed of multiple cytotoxic components (13,14) of which specific subpopulations are able to recognize different self plus haptenated target cells. These findings, therefore, demonstrate that this response is immunologically specific, and is not a result of an artifact induced by hapten conjugation to the target cells.…”

Specific Suppressor T‐Cells Immune to Antigens of the H‐2 Complex: Receptors, Clonal Structure, Genetic Restriction and Antigenic Markers

“…The CR CTL reflect the peculiarities of their specificity and can be revealed not only in the crude CTL populations but in a part of their clones (Taswell et al 1980, Guimezanes et al 1982, Plata 1982, as well as in cloned CTL precursors (Teh et al 1978). The absorptions of monospecific CTL have shown that they consist of subpopulations capable of adhering to the particular CR TC in a selective fashion (Andreev et al 1976, Geib et al 1978, Vasquez et al 1980). To study the nature of this phenomenon, the fine specificities of the CR CTL fractions isolated from the given "irrelevant" mon-olayers bearing mutant (Brondz et al 1979b) or allogeneic haplotypK {Brondz et al 1982b, c) were estimated.…”