Separate signal pathways regulate the expression of a low-temperature-induced gene in Arabidopsis thaliana (L.) Heynh.

Nordin, Kerstin; Heino, Pekka; Palva, E. Tapio

doi:10.1007/bf00016077

Cited by 221 publications

(181 citation statements)

References 39 publications

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“…In RC12B mRNA it seems that both the ABA-dependent and -independent pathways are required to achieve full cold-induced accumulation. In conclusion, RC12 genes are induced by low temperature in abil and aba2 mutants, indicating that, as described for other cold-responsive genes (Nordin et al, 1991;Gilmour and Thomashow, 1991), the accumulation of RCT2 mRNAs by low temperature is regulated by at least two different signal transduction pathways, one ABA dependent and the other ABA independent.…”

Section: Discussionmentioning

confidence: 53%

“…Their high degree of homology suggests that they probably arose by duplication of the same ancestral gene. The same origin has been proposed for four pairs of Arabidopsis low-temperature-responsive genes, in which the homologous genes are arranged in tandem in the genome; they are kinl and cor6.6/kin2 (Gilmour et al, 1992;Kurkela and Borg-Franck, 1992), corl5a and corl5b (Wilhelm and Thomashow, 1993), W78/rd29A/cor78 and W65/rd29B (Nordin et al, 1991;Horvath et al, 1993;Yamaguchi-Shinozaki and Shinozaki, 1993), and Iti45/lti29 and cor47 . It is interesting that in each case both homologous genes have diverged in their regulation by low temperature, suggesting they have different roles in cold acclimation or freezing tolerance.…”

Section: Discussionmentioning

confidence: 60%

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Two Homologous Low-Temperature-Inducible Genes from Arabidopsis Encode Highly Hydrophobic Proteins

et al. 1997

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We have characterized two related cDNAs (RCIM and RC12B) corresponding to genes from Arabidopsis thaliana, the expression of which is transiently induced by low, nonfreezing temperatures.RClZA and R C D B encode small(54 amino acids), highly hydrophobic proteins that bear two potential transmembrane domains. They show similarity to proteins encoded by genes from barley (Hordeum vulgare L.) and wheatgrass (Lophophyrum elongatum) that are regulated by different stress conditions. Their high leve1 of sequence homology (78%) and their genomic location in a single restriction fragment suggest that both genes originated as a result of a tandem duplication. However, their regulatory sequences have diverged enough to confer on them different expression patterns. Like most of the cold-inducible plant genes characterized, the expression of RC12A and RC/ZB is also promoted by abscisic acid (ABA) and dehydration but is nota general response to stress conditions, since it is not induced by salt stress or by anaerobiosis. Furthermore, low temperatures are able to induce RC12A and RC12B expression in ABA-deficient and -insensitive genetic backgrounds, indicating that both ABA-dependent and 4ndependent pathways regulate the lowtemperature responsiveness of these two genes.

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Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 60%

Two Homologous Low-Temperature-Inducible Genes from Arabidopsis Encode Highly Hydrophobic Proteins

et al. 1997

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“…It is not yet fully understood how plants can sense osmotic stress, but it is clear that abscisic acid (ABA) is involved in the expression of a number of stress-responsive genes [4]. However, studies conducted on ABA-deficient or ABA-insensitive mutants have indicated that some of these genes are expressed independently of ABA [5,6]. With regard to anoxia, it is known to quickly inhibit protein synthesis, except for a selected set of proteins called anaerobic proteins (' ANPs ') ; most of them are enzymes of glycolysis and fermentation [2].…”

Section: Introductionmentioning

confidence: 99%

Sugar/osmoticum levels modulate differential abscisic acid-independent expression of two stress-responsive sucrose synthase genes in Arabidopsis

Déjardin

Sokolov

Kleczkowski

1999

Biochemical Journal

114

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Sucrose synthase (Sus) is a key enzyme of sucrose metabolism. Two Sus-encoding genes (Sus1 and Sus2) from Arabidopsis thaliana were found to be profoundly and differentially regulated in leaves exposed to environmental stresses (cold stress, drought or O2 deficiency). Transcript levels of Sus1 increased on exposure to cold and drought, whereas Sus2 mRNA was induced specifically by O2 deficiency. Both cold and drought exposures induced the accumulation of soluble sugars and caused a decrease in leaf osmotic potential, whereas O2 deficiency was characterized by a nearly complete depletion in sugars. Feeding abscisic acid (ABA) to detached leaves or subjecting Arabidopsis ABA-deficient mutants to cold stress conditions had no effect on the expression profiles of Sus1 or Sus2, whereas feeding metabolizable sugars (sucrose or glucose) or non-metabolizable osmotica [poly(ethylene glycol), sorbitol or mannitol] mimicked the effects of osmotic stress on Sus1 expression in detached leaves. By using various sucrose/mannitol solutions, we demonstrated that Sus1 was up-regulated by a decrease in leaf osmotic potential rather than an increase in sucrose concentration itself. We suggest that Sus1 expression is regulated via an ABA-independent signal transduction pathway that is related to the perception of a decrease in leaf osmotic potential during stresses. In contrast, the expression of Sus2 was independent of sugar/osmoticum effects, suggesting the involvement of a signal transduction mechanism distinct from that regulating Sus1 expression. The differential stress-responsive regulation of Sus genes in leaves might represent part of a general cellular response to the allocation of carbohydrates during acclimation processes.

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“…9 and 10). However, studies employing the ABA-insensitive (abi) mutants of Arabidopsis indicate that there are also ABA-independent signal transduction pathways for cold-and dehydration-regulated gene expression (11,12). Indeed, the results of Yamaguchi-Shinozaki and Shinozaki (6) indicate that the C-repeat͞DRE is not responsive to ABA levels and, thus, appears to impart cold-and dehydration-regulated gene expression through an ABAindependent pathway.…”

mentioning

confidence: 99%

Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit

Stockinger

Gilmour

Thomashow

1997

Proc. Natl. Acad. Sci. U.S.A.

1,590

1,203

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Recent efforts have defined a cis-acting DNA regulatory element in plants, the C-repeat͞dehydration responsive element (DRE), that stimulates transcription in response to low temperature and water deficit. Here we report the isolation of an Arabidopsis thaliana cDNA that encodes a C-repeat͞DRE binding factor, CBF1 (C-repeat͞DRE Binding Factor 1). Analysis of the deduced CBF1 amino acid sequence indicates that the protein has a molecular mass of 24 kDa, a potential nuclear localization sequence, and a possible acidic activation domain. CBF1 also has an AP2 domain, which is a DNA-binding motif of about 60 aa present in the Arabidopsis proteins APETALA2, AINTEGUMENTA, and TINY; the tobacco ethylene response element binding proteins; and numerous other plant proteins of unknown function. The transcript levels for CBF1, which appears to be a single or low copy number gene, did not change appreciably in plants exposed to low temperature or in detached leaves subjected to water deficit. Binding of CBF1 to the C-repeat͞DRE was demonstrated in gel shift assays using recombinant CBF1 protein expressed in Escherichia coli. Moreover, expression of CBF1 in yeast was found to activate transcription of reporter genes containing the C-repeat͞DRE as an upstream activator sequence but not mutant versions of the DNA element. We conclude that CBF1 can function as a transcriptional activator that binds to the C-repeat͞DRE DNA regulatory element and, thus, is likely to have a role in cold-and dehydrationregulated gene expression in Arabidopsis.

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Separate signal pathways regulate the expression of a low-temperature-induced gene in Arabidopsis thaliana (L.) Heynh.

Cited by 221 publications

References 39 publications

Two Homologous Low-Temperature-Inducible Genes from Arabidopsis Encode Highly Hydrophobic Proteins

Two Homologous Low-Temperature-Inducible Genes from Arabidopsis Encode Highly Hydrophobic Proteins

Sugar/osmoticum levels modulate differential abscisic acid-independent expression of two stress-responsive sucrose synthase genes in Arabidopsis

Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit

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