Separation and anti‐inflammatory evaluation of phytochemical constituents from Pleurospermum candollei (Apiaceae) by high‐speed countercurrent chromatography with continuous sample load

Abstract

Pleurospermum (Apiaceae) species possess a wide range of biological properties viz. analgesic, anti‐inflammatory, antimalarial, and so on. Pleurospermum candollei (DC.) Benth. Ex C. B. Clark. is reported to cure diarrhea, gastric, respiratory, stomach, abdominal, joint, and back pain problems. In addition, it is also used for both male and female infertility. The present study deals with an efficient technique using high‐speed countercurrent chromatography for separation of chemical components from the methanol extract of P. candollei. Notably, nine main compounds namely luteolin 7‐O‐glucoside (1), oxypeucedanin hydrate (2), pabulenol (3), bergapten (4), heptadecanoic acid (5), (E)‐isoelemicin (6), trans‐asarone (7), α‐linolenic acid (8), and isoimperatorin (9) were very efficiently separated and isolated in pure form. Multiple injections were applied followed by two off‐line recycling high‐speed countercurrent chromatography. The inhibitory effect of nitric oxide production of all compounds was tested in the presence of 200 ng/mL lipopolysaccharide in RAW264.7 mice macrophage cells. The results demonstrated that compounds 7 and 8 effectively inhibited nitric oxide production, with IC50 values of 28.44 and 53.18 μM, respectively. This study thus validates the traditional claim of using P. candollei. Taken together, these findings will be useful in future research to find a potential candidate with anti‐inflammatory properties.

Pharmacological Properties of Bergapten: Mechanistic and Therapeutic Aspects

“…Ex C. B. Clark. at doses of 100 μ M evidenced a significant inhibition of NO production in RAW264.7 cells stimulated with LPS [ 32 ].…”

Comprehensive Phytochemical Profiling, Biological Activities, and Molecular Docking Studies of Pleurospermum candollei: An Insight into Potential for Natural Products Development

“…It is employed for the treatment of respiratory disorders, and the evidence also shows its good effects in pain, unconsciousness, and cerebral disorders. Many other ailments are also getting cured by the people of the Himalayan zone using this plant [14]. Stem powder of this plant has been used in joints problem and back pain in the area of Gilgit-Baltistan.…”

An efficient high‐speed counter‐current chromatography method for the preparative separation of potential antioxidants from Paeonia lactiflora Pall. combination of in vitro evaluation and molecular docking

“…Overlap of peaks will happen at a high sample loading amount which further limits the large scale separation. High-speed counter-current chromatography (HSCCC) is a fast-growing separation technique in natural products based on the liquid-liquid partition principle and has been reported for preparative separation and purification of various natural products [16][17][18][19][20]. In the most recent research, Wang et al used high-speed counter-current chromatography (HSCCC) followed by medium-pressure liquid chromatography to isolate monoterpene glycosides [21], which was able to achieve a high recovery yield of 95%.…”

“…Overlap of peaks will happen at a high sample loading amount which further limits the large scale separation. High‐speed counter‐current chromatography (HSCCC) is a fast‐growing separation technique in natural products based on the liquid‐liquid partition principle and has been reported for preparative separation and purification of various natural products [16–20]. In the most recent research, Wang et al.…”