Separation and characterisation of products of the reaction between propionamide and formaldehyde

Abstract

Propionamide-formaldehyde reaction products have been characterised using micellar electrokinetic chromatography (MEKC), HPLC-electrospray-MS (HPLC-ES-MS) and time-of-flight-MS (TOF-MS). HPLC-MS-MS was used to distinguish bel',,veen isomeric species. The MEKC separation of all reaction products was obtained with good resolution and efficiency. Comparison of water-micelle distribution constants (PMEKC) for propionamide-formaldehyde with those for caprolactam-formaldehyde reaction products suggests that the concentration of sodium dodecyl sulfate appropriate for use in MEKC is inversely related to PMEKO Quantification of all species containing one or I',,vo amide unitswas achieved using standard calibration, mass balance and the assumption of identical absorption coefficients for the same functional groups in monomers and dimers.

Reversed-phase high-performance liquid chromatographic separation of some 2-arylpropionic acids using vancomycin as chiral stationary phase

“…Currently, a number of chiral stationary phases have been described in the literature for HPLC, which have been shown to be efficient for the separation of enantiomeric drugs and other compounds [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22]. However, there is at present no chiral stationary phase in HPLC that is capable of separating all classes of drugs, so that analysts have to select a convenient phase for a particular separation from a large selection of chiral stationary phases.…”