Separation and detection of polar cuticular components from Oriental tobacco leaf by integration of normal‐phase liquid chromatography fractionation with reversed‐phase liquid chromatography‐mass spectrometry

Ding, Li; Xie, Fuwei; Xu, Guowang; Liu, Kejian; Wang, Sheng; Xie, Jianping

doi:10.1002/jssc.201000536

Cited by 9 publications

(6 citation statements)

References 34 publications

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“…There are several reports on the isolation and structural characterization of different types of SCE homologues from Nicotiana by silica gel column chromatography or HPTLC combined with spectroscopic methods, , LC–MS, − and LC–MS n . , The SCEs in the cuticular waxes of green tobacco are the major components of Turkish tobacco (N. tabacum) .…”

Section: Metabolite Classes: Structures and Functionsmentioning

confidence: 99%

Ecological Roles and Biological Activities of Specialized Metabolites from the GenusNicotiana

Jassbi¹,

Zare²,

Asadollahi³

et al. 2017

Chem. Rev.

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Species of Nicotiana grow naturally in different parts of the world and have long been used both medicinally and recreationally by human societies. More recently in our history, Nicotiana tabacum has attracted interest as one of the most economically important industrial crops. Nicotiana species are frequently investigated for their bioactive natural products, and the ecological role of their specialized metabolites in responses to abiotic stress or biotic stress factors like pathogens and herbivores. The interest of tobacco companies in genetic information as well as the success of a few wild tobacco species as experimental model organisms have resulted in growing knowledge about the molecular biology and ecology of these plants and functional studies of the plant's natural products. Although a large number of reviews and books on biologically active natural products already exists, mostly from N. tabacum, we focus our attention on the ecological roles and biological activity of natural products, versus products from cured and processed material, in this Review. The studied compounds include alkaloids, aromatic compounds, flavonoids, volatiles, sesquiterpenoids, diterpenes alcohols, and sugar esters from trichomes of the plants, and recently characterized acyclic hydroxygeranyllinalool diterpene glycosides (HGL-DTGs). In this Review (1800s-2017), we describe the above-mentioned classes of natural products, emphasizing their biological activities and functions as they have been determined either in bioassay-guided purification approaches or in bioassays with plants in which the expression of specific biosynthetic genes has been genetically manipulated. Additionally, a review on the history, taxonomy, ecology, and medicinal application of different Nicotiana species growing around the globe presented in this Review may be of interest for pharmacognosists, natural products, and ecological chemists.

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Section: Metabolite Classes: Structures and Functionsmentioning

confidence: 99%

Ecological Roles and Biological Activities of Specialized Metabolites from the GenusNicotiana

Jassbi¹,

Zare²,

Asadollahi³

et al. 2017

Chem. Rev.

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“…Acetonitrile, dichloromethane and ethyl acetate were commonly chosen for extraction of SEs from tobacco. 10,12,24 Herein, the extraction efficiency of three kinds of solvent was first investigated. The average values of peak areas obtained for five groups of sucrose esters from three replicates were used to evaluate extraction efficiencies.…”

Section: Optimization Of Sample Preparationmentioning

confidence: 99%

Separation and Analysis of Sucrose Esters in Tobacco by Online Liquid Chromatography-Gas Chroinatography/Mass Spectrometry

Huang

Sha

et al. 2018

ANAL. SCI.

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In this work, a strategy of in-series combination of ultrasound-assisted extraction and online LC-GC/MS was constructed for effective separation and analysis of sucrose esters in tobacco. Sucrose esters were first extracted by ultrasound-assisted extraction with high efficiency and easyhandling. Online LC-GC/MS was then applied for sucrose ester clean-up and analysis. To better evaluate the effectiveness of this strategy, we limited our focus to five groups of sucrose ester isomers. Each group differed in mass from the next by 14 Da. The obtained coefficient of the calibration curve was 0.9986. Limit of detection (LOD) and limit of quantitation (LOQ) were 0.05 and 0.16 μg/ mL, respectively. The recovery was above 90% and the reproducibility was below 4%. This strategy was subsequently applied to the comparison of relative amounts of five groups of sucrose esters extracted from three different parts of aromatic tobacco. The satisfactory performance indicated that this strategy has great prospect for the rapid and high-throughput analysis of sucrose esters in tobacco.

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“…The California Department of Public Health reports that African American smokers have the greatest prevalence of menthol cigarette use (82.6%). In a few nations, flavour compounds in electronic cigarette (EC) fluids that may harm human health have been examined [20]. Alternatives to traditional cigarettes (CC) have been offered to the public in recent years.…”

Section: Introductionmentioning

confidence: 99%

Extraction and Characterisation of Flavoured Tobacco by GC-MS Studies and Validation of Results by GC-FID to Investigate Hazardous Flavour Ingredients

Patil¹,

Naik²,

Sonawane³

et al. 2022

EJNFS

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Flavoured tobacco is mainly consumed in India and neighbouring countries like Pakistan, Afghanistan, and Nepal and the hazards are known. Considering the need to identify such flavouring ingredients and a simple analytical method was required to quantify such favouring ingredients and hazardous / allergens, we selected top brands available in India for investigation. We simply extracted the ingredients by triturating with Diethyl Ether, evaporating solvent ether and reconstituting the extract in Acetone & Ethanol for GC-MS & GC-FID work respectively. The flavour ingredients were identified, and hazardous ingredients, viz. Diethyl Phthalate was identified. It was found around 2.5% to 3.0%. The GC-MS method was validated with GC-FID analysis with Linearity, LOD & LOQ study.

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Separation and detection of polar cuticular components from Oriental tobacco leaf by integration of normal‐phase liquid chromatography fractionation with reversed‐phase liquid chromatography‐mass spectrometry

Cited by 9 publications

References 34 publications

Ecological Roles and Biological Activities of Specialized Metabolites from the GenusNicotiana

Ecological Roles and Biological Activities of Specialized Metabolites from the GenusNicotiana

Separation and Analysis of Sucrose Esters in Tobacco by Online Liquid Chromatography-Gas Chroinatography/Mass Spectrometry

Extraction and Characterisation of Flavoured Tobacco by GC-MS Studies and Validation of Results by GC-FID to Investigate Hazardous Flavour Ingredients

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