Separation and determination of flavonoids in three traditional chinese medicines by capillary electrophoresis with amperometric detection

Wang, Wei; Lin, Ping; Ma, Lihong; Xu, Kaixuan; Lin, Xiuli

doi:10.1002/jssc.201501287

Cited by 54 publications

(27 citation statements)

References 31 publications

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“…23 Many studies have revealed that rutin is good for human health, such as antibacterial, antiulcer, anti-inflammatory, antiarrhythmia, antitumor, protective and expanding blood vessel. 24,25 Up to now, lots of analytical methods have been applied for the determination of rutin, including chemiluminescence, 26,27 high-performance liquid chromatography (HPLC), 28,29 capillary electrophoresis spectrophotometry, 30,31 UV-Vis spectrophotometry 32 and electrochemical methods. [33][34][35][36] In this work, Cu-doped carbon quantum dots (Cu-CQDs) were prepared by a facile one-step pyrolytic synthesis using Na 2 [Cu(EDTA)] as precursor.…”

Section: Introductionmentioning

confidence: 99%

Fluorescent Sensors Based on Cu-Doped Carbon Quantum Dots for the Detection of Rutin

Wang¹,

Shang²,

Zheng³

et al. 2018

J. Braz. Chem. Soc.

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In this paper, Cu-doped carbon quantum dots (Cu-CQDs) were prepared by carrying out thermolysis of Na 2 [Cu(EDTA)] and hydroxylamine hydrochloride at 300 °C for 2 h. The asprepared Cu-CQDs were characterized by UV-Vis, Fourier transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD). The Cu-CQDs exhibited good fluorescence property, high stability, excitationdependent emission behavior and a quantum yield of 9.8%. More significantly, the synthesized Cu-CQDs were developed for sensing rutin (RT) based on the fluorescence quenching of the prepared Cu-CQDs. A good linear relationship between the fluorescence quenching of Cu-CQDs and different concentrations of RT was obtained in the range of 0.1-15 μg mL -1 with a correlation coefficient of 0.9959. The detection limit was as low as 0.05 μg mL -1 (signal-to-noise ratio, S/N = 3). The possible mechanism of fluorescence quenching was explored, which was proved to be inner filter effect. Importantly, the proposed method was successfully applied in the detection of RT tablet samples and with satisfactory results, showing the practical applications.Keywords: Cu-doped carbon quantum dots, rutin, Na 2 [Cu(EDTA)], fluorescence sensors Rutin tablet samples pretreatment 10 pieces of rutin tablets were ground to powder in a mortar, and 10 mg of the powder was dissolved in 25 mL of ethanol. Recovery experiments were performed by adding different concentrations of rutin to the treated samples. Results and Discussion Characterization of Cu-CQDsThe optical properties of the Cu-CQD were investigated by UV-Vis absorption and fluorescence emission spectra. The UV-Vis spectrum (Figure 2a) showed two shoulders at Fluorescent Sensors Based on Cu-Doped Carbon Quantum Dots for the Detection of Rutin J. Braz. Chem. Soc. 990

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Section: Introductionmentioning

confidence: 99%

Fluorescent Sensors Based on Cu-Doped Carbon Quantum Dots for the Detection of Rutin

Wang¹,

Shang²,

Zheng³

et al. 2018

J. Braz. Chem. Soc.

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“…Among these chemical compositions, flavonoids are the main active constituents present in the plant, which exhibited anxiolytic and antidepressant activity [20, 21]. Several methods have been developed for the determination of quercetin, luteolin, or apigenin in the plants, foods, and biological samples [22–25]. However, to our knowledge, there has been no report on the simultaneous determination of quercetin, luteolin, and apigenin in animal plasma after oral administration of M. chamomilla extracts.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination and Pharmacokinetic Study of Quercetin, Luteolin, and Apigenin in Rat Plasma after Oral Administration of Matricaria chamomilla L. Extract by HPLC‐UV

Dong

Wei

Yin

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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A simple and sensitive HPLC-UV method has been developed for the simultaneous determination of quercetin, luteolin, and apigenin in rat plasma after oral administration of Matricaria chamomilla L. extract. The flow rate was set at 1.0 ml/min and the detection wavelength was kept at 350 nm. The calibration curves were linear in the range of 0.11–11.36 μg/ml for quercetin, 0.11–11.20 μg/ml for luteolin, and 0.11–10.60 μg/ml for apigenin, respectively. The intraday and interday precisions (RSD) were less than 8.32 and 8.81%, respectively. The lower limits of quantification (LLOQ) of the three compounds were 0.11 μg/ml. The mean recoveries for quercetin, luteolin, and apigenin were 99.11, 95.62, and 95.21%, respectively. Stability studies demonstrated that the three compounds were stable in the preparation and analytical process. The maximum plasma concentration (Cmax) was 0.29 ± 0.06, 3.04 ± 0.60, and 0.42 ± 0.10 μg/ml, respectively. The time to reach the maximum plasma concentration (Tmax) was 0.79 ± 0.25, 0.42 ± 0.09, and 0.51 ± 0.13 h, respectively. The validated method was successfully applied to investigate the pharmacokinetics study of quercetin, luteolin, and apigenin in rat plasma after oral administration of M. chamomilla extract.

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“…The LODs were ranged from 0.46 to 0.85 μM. . In reality, the materials of the detection electrodes employed in CE‐AD strongly influenced their performances (sensitivity and reproducibility).…”

Section: Methods Developmentmentioning

confidence: 99%

Capillary electrophoresis in phytochemical analysis (2014–2017)

Zhou

Zhang

2018

Separation Science Plus

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This updated review is focused on the applications of CE in phytochemical analysis during the period of 2014 to April 2018 (about 130 papers were included). The article mainly consists of three main sections, including sample pretreatment (extraction and on‐line pre‐concentration), method development (separation and detection) and applications in quality evaluation of medicinal plants, screening of active compounds (enzyme inhibitors) and studying interaction between phytochemicals and bio‐macromolecules.

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Separation and determination of flavonoids in three traditional chinese medicines by capillary electrophoresis with amperometric detection

Cited by 54 publications

References 31 publications

Fluorescent Sensors Based on Cu-Doped Carbon Quantum Dots for the Detection of Rutin

Fluorescent Sensors Based on Cu-Doped Carbon Quantum Dots for the Detection of Rutin

Simultaneous Determination and Pharmacokinetic Study of Quercetin, Luteolin, and Apigenin in Rat Plasma after Oral Administration of Matricaria chamomilla L. Extract by HPLC‐UV

Capillary electrophoresis in phytochemical analysis (2014–2017)

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