Separation and Determination of Isoflavonoids in Several Kudzu Samples by High‐Performance Capillary Electrophoresis (HPCE)

Abstract

Pueraria lobata is a rich source of isoflavonoids. The detection and identification of isoflavonoid components from root, stem, leaf, callus and cell samples, is very important for the best, safest and most efficacious use of kudzu as a medicinal plant, and for the studies on quantitative analysis in the secondary metabolism of isoflavonoids. In this paper, a simple, rapid and precise high-performance capillary electrophoresis (HPCE) method with diode array detection (DAD) has been developed for separation and determination of isoflavonoids in several kudzu samples. The isoflavonoids could be well separated within 15 min in a 40 cm length capillary at a separation voltage of 15kV in a 30 mmol L(-1) borax buffer (pH9.29), and this proposed method demonstrated excellent reproducibility and accuracy with relative standard deviations of less than 5% for isoflavonoid content (n = 5) of different kudzu samples. The relationship between peak areas and isoflavone concentrations, in a specified working range with linear response, was determined by first-order polynomial regression over the range 0.05-0.5 mg mL(-1) for puerarin and 2.5-50 microg mL(-1) for 3'-methoxypuerarin, daidzin and daidzein, respectively, and quantitative evaluation of those four main isoflavonoid components was determined by ultraviolet absorption at lambda = 192 nm. The differences were also illustrated by comparison of the determination of isoflavonoid components from kudzu root, stem, leaf samples and plant tissue cultures in vitro.

Molecular Cloning and Functional Characterization of Two Divergent 4-Coumarate : Coenzyme A Ligases from Kudzu (<i>Pueraria lobata</i>)

“…The Transcript of Pl4CL1 but Not Pl4CL2 Was Related to the Biosynthesis of Puerarin As previously reported, 35,36) the accumulation of puerarin was mostly detected in the roots compared to other organs (Fig. 5a).…”

Optimisation of Ultrasound‐assisted Extraction of Puerarin and Total Isoflavones from Puerariae Lobatae Radix (Pueraria lobata (Wild.) Ohwi) with Response Surface Methodology

“…The results are shown in Table together with the amounts of puerarin and total isoflavones extracted under the optimised conditions. These extracted amounts were higher than those obtained in previous reports (Jiang et al ., ; Zhang et al ., ; Fang et al ., ; Cherdshewasart et al ., ; Lee and Lin, ; Du et al ., ), which further demonstrates that the response model adequately reflected the predicted optimisation.…”

Effects of a surface topography composite with puerariae radix on human STRO-1-positive stem cells

“…This traditional Chinese medicine has been used for the treatment of various diseases and to promote bone healing for millenia and recent data suggest beneficial activity (16)(17)(18)(19)(20). The active ingredients have been identified as the isoflavones: puerarin, daidzin and daidzein, which have estrogenic activity (21)(22)(23)(24). Puerariae radix has shown positive therapeutic effects in animal studies (25)(26)(27) and in cell culture studies, Puerariae radix was shown to induce osteogenic activity (20,(28)(29)(30)(31).…”