Separation and determination of l-tyrosine and its metabolites by capillary zone electrophoresis with a wall-jet amperometric detection

Huang, Ying; Jiang, Xiuyan; Wang, Wei; Duan, Jianping; Chen, Guonan

doi:10.1016/j.talanta.2006.03.009

Cited by 56 publications

(21 citation statements)

References 19 publications

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“…When the concentration is lowered to 8 Â 10 À9 M, the anodic peak is still discernible (S/N ¼ 3). In comparison with other voltammetric methods reported [15 -19] 6 ]/GCE, the RSD of peak current is 3.4%.…”

Section: Calibration Curve and Reproducibilitymentioning

confidence: 68%

“…Hence, Cu 2þ ion is chosen in the following experiments. As shown in Figure 2B, 6 ] is more acceptable.…”

Section: Influence Of Metal Ions and Ionic Liquidsmentioning

confidence: 90%

“…The assay of Tyr is required in clinic and food fields and so on. The approaches for Tyr determination are mainly high-performance liquid chromatography [3], chromatography-mass spectrometry [4,5], and capillary electrophoresis [6,7]. However, these methods suffer from either slow detection speed or high expense.…”

Section: Introductionmentioning

confidence: 99%

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Improved Voltammetric Response of L‐Tyrosine on Multiwalled Carbon Nanotubes‐Ionic Liquid Composite Coated Glassy Electrodes in the Presence of Cupric Ion

et al. 2008

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l-Tyrosine can exhibit a small anodic peak on multiwalled carbon nanotubes (MWCNTs) coated glassy carbon electrodes (GCE). At pH 5.5 its peak potential is 0.70 V (vs. SCE). When an ionic liquid (i.e., 1-octyl-3-methylimidazolium hexafluorophosphate, [omim][PF 6 ]) is introduced on the MWCNT coat, the peak becomes bigger. Furthermore, in the presence of Cu 2þ ion the anodic peak of l-tyrosine increases further due to the formation of Cu 2þ -l-tyrosine complex, while the peak potential keeps unchanged. Therefore, a sensitive voltammetry based on the oxidation of Cu 2þ -l-tyrosine complex on MWCNTs-[omim] [PF 6 ] composite coated electrode is developed for ltyrosine. Under the optimized conditions, the anodic peak current is linear to l-tyrosine concentration in the range of 1 Â 10 À8 -5 Â 10 À6 M, and the detection limit is 8 Â 10 À9 M. The modified electrode shows good reproducibility and stability. In addition, the voltammetric behavior of other amino acids is explored. It is found that among them tryptophan (Trp) and histidine (His) can also produce sensitive anodic peak under same experimental conditions, and their detection limits are 4 Â 10 À9 M and 4 Â 10 À6 M, respectively.

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Section: Calibration Curve and Reproducibilitymentioning

confidence: 68%

“…Hence, Cu 2þ ion is chosen in the following experiments. As shown in Figure 2B, 6 ] is more acceptable.…”

Section: Influence Of Metal Ions and Ionic Liquidsmentioning

confidence: 90%

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Improved Voltammetric Response of L‐Tyrosine on Multiwalled Carbon Nanotubes‐Ionic Liquid Composite Coated Glassy Electrodes in the Presence of Cupric Ion

et al. 2008

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“…Easy detection of these metabolites would be helpful in assessing medicinal metabolism in the body. Using CE with amperometric detection, Chen and co-workers [102] detected seven such metabolites from a urine sample. In addition to urine, saliva can be a sample source for forensic analysis.…”

Section: Ce In Metabolomicsmentioning

confidence: 99%

CE at the omics level: Towards systems biology – An update

Song

Babar

et al. 2007

Electrophoresis

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This review provides an updated overview of recent developments and applications of CE based on previously published reports in the field of omic research. The increased number of published articles on omics shows that the field is growing and attracting the attention of many life science researchers. Due to developments in the omics sciences, many researchers have been studying systems biology, in which biological events in organisms are systematically interpreted through the combination of complex measurements from various methods resulting in high-throughput data. Given the challenges of such complex forms of analysis, CE is a strong candidate for generating omics data useful for acquiring the qualitative and quantitative knowledge necessary for systems-level investigation. By emphasizing CE for systems biology, this review will discuss and focus on the applicability of CE to systems-based analytical data at the genomic, transcriptomic, proteomic, and metabolomic levels from 2005 to the present.

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“…Because of the physiological and clinical significance, chemistry of pHPP has been a topic of constant interest. The methods for determination of pHPP included flow injection spectrophotometry combined with single chip microcomputer [3], fluorescence quenching [4], GC [5][6], HPLC [7], CE [8][9][10], and electrochemical technique [1]. The Keto-enol tautomerism of pHPP was early investigated by Chi et al, using electrochemical method and UV spectrometry [1].…”

Section: Introductionmentioning

confidence: 99%

Characterization of keto‐enol tautomerism of p‐hydroxyphenylpyruvic acid using CE with amperometric detection and spectrometric analysis

Huang¹,

Zhang²,

LiangJun³

et al. 2009

J of Separation Science

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A high-performance CE with amperometric detection (CE-AD) was employed for the kinetic study of keto-enol tautomerism of p-hydroxyphenylpyruvic acid (pHPP). Several factors (concentration of b-cyclodextrin (b-CD), concentration and pH of running buffer, separation voltage and injection time) affecting CE-AD were investigated and separation conditions were optimized. The kinetics of pHPP was performed in water solution and phosphate solution under different pH and temperature, the homologous ketonization rate constants and half-life were obtained. Also, the activation energy was calculated according to the rate constants under different temperature. The experimental results indicated that b-CD played an important role in the separation, therefore UV spectrometric method was applied for the study of complexation interaction between ketonic pHPP and b-CD. The results indicated that the stoichiometric ratio of the pHPP-b-CD complex was 1:1 and formation constant was determined. The obtained kinetic results are in correspondence with those reported by our group with CE-UV.

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Separation and determination of l-tyrosine and its metabolites by capillary zone electrophoresis with a wall-jet amperometric detection

Cited by 56 publications

References 19 publications

Improved Voltammetric Response of L‐Tyrosine on Multiwalled Carbon Nanotubes‐Ionic Liquid Composite Coated Glassy Electrodes in the Presence of Cupric Ion

Improved Voltammetric Response of L‐Tyrosine on Multiwalled Carbon Nanotubes‐Ionic Liquid Composite Coated Glassy Electrodes in the Presence of Cupric Ion

CE at the omics level: Towards systems biology – An update

Characterization of keto‐enol tautomerism of p‐hydroxyphenylpyruvic acid using CE with amperometric detection and spectrometric analysis

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