Separation and determination of nanogram amounts of inorganic arsenic and methylarsenic compounds

Braman, Robert S.; Johnson, David L.; Foreback, Craig C.; Ammons, James M.; Bricker, Joseph L.

doi:10.1021/ac50012a029

Cited by 236 publications

(67 citation statements)

References 19 publications

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“…However, MMA(V) is not a phosphate analogue (Delnomdedieu et al, 1995), and thus it is unlikely to be transported into cells by phosphate carriers. MMA(V) has a pK a1 of 2.6 (Braman et al, 1977), and would be expected to be ionized at physiological pH, thus decreasing its ability to diffuse passively across membranes. Arsenite (trivalent iAs) is transported across membranes by aquaglyceroporins and hexose permeases (Liu et al, 2002(Liu et al, , 2004a(Liu et al, , 2004b.…”

Section: Discussionmentioning

confidence: 99%

Tissue dosimetry, metabolism and excretion of pentavalent and trivalent monomethylated arsenic in mice after oral administration

Hughes

Devesa

Adair

et al. 2005

Toxicology and Applied Pharmacology

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Exposure to monomethylarsonic acid (MMA(V)) and monomethylarsonous acid (MMA(III)) can result from their formation as metabolites of inorganic arsenic and by the use of the sodium salts of MMA(V) as herbicides. This study compared the disposition of MMA(V) and MMA(III) in adult female B6C3F1 mice. Mice were gavaged po with MMA(V), either unlabeled or labeled with 14 C at two dose levels (0.4 or 40 mg As/kg). Other mice were dosed po with unlabeled MMA(III) at one dose level (0.4 mg As/kg). Mice were housed in metabolism cages for collection of excreta and sacrificed serially over 24 h for collection of tissues. MMA(V)-derived radioactivity was rapidly absorbed, distributed and excreted. By 8 h post-exposure, 80% of both doses of MMA(V) were eliminated in urine and feces. Absorption of MMA(V) was dose dependent; that is, there was less than a 100-fold difference between the two dose levels in the area under the curves for the concentration-time profiles of arsenic in blood and major organs. In addition, urinary excretion of MMA(V)-derived radioactivity in the low dose group was significantly greater (P < 0.05) than in the high dose group. Conversely, fecal excretion of MMA(V)-derived radioactivity was significantly greater (P < 0.05) in the high dose group than in the low dose group. Speciation of arsenic by hydride generation-atomic absorption spectrometry in urine and tissues of mice administered MMA(V) or MMA(III) found that methylation of MMA(V) was limited while the methylation of MMA(III) was extensive. Less than 10% of the dose excreted in urine of MMA(V)-treated mice was in the form of methylated products, whereas it was greater than 90% for MMA(III)-treated mice. In MMA(V)-treated mice, 25% or less of the tissue arsenic was in the form of dimethylarsenic, whereas in MMA (III)-treated mice, 75% or more of the tissue arsenic was in the form of dimethylarsenic. Based on urinary analysis, administered dose of MMA(V) did not affect the level of its metabolites excreted. In the tested range, dose affects the absorption, distribution and route of excretion of MMA(V) but not its metabolism.

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Section: Discussionmentioning

confidence: 99%

Tissue dosimetry, metabolism and excretion of pentavalent and trivalent monomethylated arsenic in mice after oral administration

Hughes

Devesa

Adair

et al. 2005

Toxicology and Applied Pharmacology

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“…The CT-HG-AAS is widely used in the analysis of As compound, such as As(V), As(III), MMAA(V) and DMAA(V) (Braman et al, 1977;Hasegawa et al, 1994). In this technique, As is reduced to hydride forms such as arsine, and is then transported to the atomization part at boiling point.…”

Section: P a G Ementioning

confidence: 99%

Seasonal changes of arsenic speciation in lake waters in relation to eutrophication

Hasegawa

Rahman

Kitahara

et al. 2010

Science of The Total Environment

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“…A sample of a Standard Reference Material (SRM) from the National Research Council of Canada (NRCC), SLEW-2 was concentrated and analyzed along with each group of samples to check accuracy. Arsenic was analyzed by hydride generation, cryogenic trapping, chromatographic separations, and detection using a hydrogen-burning quartz cuvette (Andreae 1977;Braman et al 1977;Riedel 1993) (Van Loon 1985). The resulting digestates were analyzed for As, Cu, Cd, Ni, and Zn by the same techniques cited for dissolved trace elements.…”

mentioning

confidence: 99%

The interrelationships among trace element cycling, nutrient loading, and system complexity in estuaries: A mesocosm study

Riedel¹,

Sanders

2003

Estuaries

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Biogeochemical interactions between a suite of trace elements and nutrients were examined in a series of experimental mesocosm experiments to understand how multiple stressors affect estuarine environments and how these effects are modified by the complexity of the system used to examine them. Experimental treatment included additions of nutrients and trace elements separately and combined, along with a gradient in experimental system complexity. Eight mesocosm experiments were carried out from 1996 through 1998. Increased nutrients generally decreased dissolved trace element concentrations, in large part through an increase in phytoplankton biomass, but also by increasing the concentration of metals in the particles. Trace element additions increased dissolved nutrients by decreasing phytoplankton biomass. The presence of sediments reduced both dissolved trace element and nutrient concentrations. Other complexity treatments had weaker effects on both dissolved nutrients and trace elements. Many of the observed effects appeared to be seasonal, occurring only in spring, or their magnitude was greater in spring. This may be linked to a change from phosphorus to nitrogen limitation that often occurs in the Patuxent River estuary in the late spring or early summer period.

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Separation and determination of nanogram amounts of inorganic arsenic and methylarsenic compounds

Cited by 236 publications

References 19 publications

Tissue dosimetry, metabolism and excretion of pentavalent and trivalent monomethylated arsenic in mice after oral administration

Tissue dosimetry, metabolism and excretion of pentavalent and trivalent monomethylated arsenic in mice after oral administration

Seasonal changes of arsenic speciation in lake waters in relation to eutrophication

The interrelationships among trace element cycling, nutrient loading, and system complexity in estuaries: A mesocosm study

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