Separation and identification of organic and organometallic compounds by use of a liquid chromatography–particle beam-glow discharge mass spectrometry combination

Gibeau, Terri E.; Marcus, R. Kenneth

doi:10.1016/s0021-9673(01)00595-7

Cited by 19 publications

(20 citation statements)

References 37 publications

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“…Typically, GD‐generated mass spectra of organic compounds yield classical EI type spectra [M + . ],23,24 while APCI techniques give protonated molecules ([M+H] + ions) through gas‐phase acid/base reactions. The different responses observed here in the glow discharge spectra for DNA and RNA species must be due to the differing chemistries of these compounds that are in fact non‐protonated at neutral pH values.…”

Section: Resultsmentioning

confidence: 99%

“…The desire to make the GD source a multifaceted mass spectrometric analysis tool has led researchers to explore the possibilities of using the source for analysis of both inorganic and organic solutions. To this end, Marcus and co‐workers have demonstrated the use of the particle beam (PB) as a means of introducing liquid‐phase samples into GD sources 19–24. The PB interface, originally applied in the MAGIC/LC/MS approach of Willoughby and Browner,25 provides a means of introducing LC effluents into low‐pressure sources (i.e., electron impact and chemical ionization) while maintaining chromatographic characteristics such as retention and elution quality 26,27.…”

mentioning

confidence: 99%

“…As such, the PB interface is well suited for the introduction of solution‐phase analytes into GD sources. The coupling of the particle beam interface to a glow discharge mass spectrometry (PB‐GDMS) ion source produces a high level of information content for both molecular and elemental species in solution analyses 23,24. Specifically, the mass spectra of a wide variety of compounds (e.g., selenoamino acids, hormones, and organometallics) are very EI‐like in nature, yielding molecular ions and easily identified fragmentation patterns.…”

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confidence: 99%

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Particle beam glow discharge mass spectrometry: spectral characteristics of nucleobases

Davis

Venzie

Willis

et al. 2003

Rapid Comm Mass Spectrometry

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Use of a particle beam glow discharge (PB-GD) source for mass spectrometric determinations of deoxy- and ribonucleosides and nucleotides is described. Use of this combination of sample introduction and ion source decouples the vaporization and ionization steps, leading to very simple spectral structure. The mass spectra of these compounds are EI-like in nature, with clearly identified molecular ions and fragmentation patterns that are easily rationalized. The PB-GDMS combination can be operated in a flow injection mode wherein the analyte is injected directly into the solvent flow, or can also be coupled to a high-performance liquid chromatography (HPLC) system allowing LC/MS analysis of mixtures. Mass spectra obtained for nucleic acid bases, nucleosides, and nucleotides are readily obtained with injections of low-nanomole quantities. Representative PB-GDMS spectra for deoxy- and ribonucleosides, nucleotides, and mixed-base oligonucleotides are presented to demonstrate the capabilities of the GD source. Characteristic fragmentation peaks from the spectra of adenine, cytosine, guanine, and thymine were identified in 22-base sequences of single-stranded DNA. The PB-GD source is capable of producing spectra that may be used to identify the individual bases present in mixed-base DNA and RNA fragments.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Particle beam glow discharge mass spectrometry: spectral characteristics of nucleobases

Davis

Venzie

Willis

et al. 2003

Rapid Comm Mass Spectrometry

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“…One way to reduce the solvent load is by employing highly efficient desolvation, such as is possible with a particle beam (PB) interface [201]. In this way, Marcus et al [185,195,202] have shown that liquid chromatographic separations can be coupled with GDMS quite simply and at a wide range of flow rates and solvent polarities. Fig.…”

Section: Reduced-pressure Plasmas As Tunable Ion Sourcesmentioning

confidence: 99%

“…Gibeau and Marcus [185] have shown that LC/PB-GD-MS could be used for analysis of inorganic and organic compounds, with limits of detection of 5.8-6.1 g/L for Fe, Ag, and Cs, and 13 g/L for caffeine as the molecular ion. This system has been employed for the analysis of several polycyclic aromatic hydrocarbons, steroids, selenoamino acids, and alkyllead compounds [202], for arsenic speciation [195], and recently for the investigation of individual nucleobases in single-stranded DNA and RNA fragments. In this last application, mass spectra of the four bases were found to be very similar to those observed in EI spectra (radical cation), and individual bases in 22mer oligomer strands were detected at the nanomolar level.…”

Section: Reduced-pressure Plasmas As Tunable Ion Sourcesmentioning

confidence: 99%

Plasma-source mass spectrometry for speciation analysis: state-of-the-art

Ray¹,

Andrade²,

Gamez³

et al. 2004

Journal of Chromatography A

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Current and emerging capabilities of plasma-source mass spectrometry (PS-MS) as it is employed for elemental speciation analysis are reviewed. Fundamental concepts and their advantageous aspects, experimental conditions, and analytical performance are described and illustrated by recent examples from the literature. Novel instrumentation, techniques, and strategies for inductively-coupled plasma mass spectrometry (ICP-MS), microwave-induced plasma (MIP) mass spectrometry, glow-discharge (GD) mass spectrometry, and electrospray ionization (ESI), among others, are described. The use of ionization sources that provide tunable ionization, others that can be modulated between different sets of operating conditions, and others used in parallel is also examined.

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Current Awareness

2001

J. Mass Spectrom.

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of mass spectrometry. Each bibliography is divided into 11 sections: 1 Books, Reviews & Symposia; 2 Instrumental Techniques & Methods; 3 Gas Phase Ion Chemistry; 4 Biology/Biochemistry: Amino Acids, Peptides & Proteins; Carbohydrates; Lipids; Nucleic Acids; 5 Pharmacology/Toxicology; 6 Natural Products; 7 Analysis of Organic Compounds; 8 Analysis of Inorganics/Organometallics; 9 Surface Analysis; 10 Environmental Analysis; 11 Elemental Analysis. Within each section, articles are listed in alphabetical order with respect to author (4 Weeks journals ‐ Search completed at 13th. June. 2001)

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Separation and identification of organic and organometallic compounds by use of a liquid chromatography–particle beam-glow discharge mass spectrometry combination

Cited by 19 publications

References 37 publications

Particle beam glow discharge mass spectrometry: spectral characteristics of nucleobases

Particle beam glow discharge mass spectrometry: spectral characteristics of nucleobases

Plasma-source mass spectrometry for speciation analysis: state-of-the-art

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