Separation and identification of trifluralin metabolites by open-tubular liquid chromatography/negative chemical ionization mass spectrometry

Abstract

The utility of combined open-tubular liquid chromatography/negative ion chemical ionization (NCI) mass spectrometry for the separation and analysis of trifluralin, a commonly used pre-emergence herbicide, and seven of its metabolites has been demonstrated. The NCI mass spectra for these compounds are reported. Some of the NCI mass spectra were obtained from as little as 50 pg of analyte.

Electron capture mass spectrometry of organic environmental contaminants

“…An added degree of selectivity was gained when using GC/EC-MS; SIM of the M-' ion for trifluralin and ethalfluralin can be used to differentiate the analytes of interest from other co-eluting electrophilic species. Trifluralin and its metabolites in soil were also analyzed by De Wit et al using liquid chromatographic mass spectrometry (108). The authors successfully interfaced a liquid chromatograph to a mass spectrometer operated under methane electron capture ionization conditions (109).…”

Nanoscale separations combined with electrospray ionization mass spectrometry: Sulfonamide determination

“…Applications of microbore LC/electrospray ionization (ESI)/MS include the determination of peptide and protein standards [22,26,30,[34][35][36][37][38][39]; tryptic digests [26,27,34,[37][38][39]; sulfonated azo dyes [22,23]; steroid sulfates [25,28]; oligosaccharides, glycopeptides, and glycoproteins [31]; synthetic estrogens [22,24]; marine toxins (29,32,33], adenosine; and methyl red [24]. Our interest in this trend has been in the development of miniaturized separation techni ques in LC, i.e., nanoscale LC separation techniques, in conjunction with mass spectrometric detection [40][41][42][43][44][45][46][47][48][49][50][51]. These nanoscale separation techniques are characterized by liquid flow rates that are in the nanoliter per minute rather than microliter per minute range, and include packed capillary LC (50-or 75-l£m i.d .…”

Optimization of a coaxial continuous flow fast atom bombardment interface between capillary liquid chromatography and magnetic sector mass spectrometry for the analysis of biomolecules

“…We have successfully interfaced OTLC with both electron impact and chemical ionization mass spectrometry (11,12), but these approaches so far have limited utility for more polar and higher molecular weight analytes. Our interest in extending the application of OTLC/MS to the analysis of biopolymers led us to investigate methods for coupling OTLC with .…”