Separation and Identification of Two Fungal Strains in Stored Maize

Abstract

Abstract:The microbiological spoilage of stored grain is important for human health and economic loss. Two major predominant grain spoilage fungi (M15 and M16) were isolated from maize from a granary in China. Morphological observation of the fungal isolates, analysis of their ITS sequences and construction of their phylogenetic trees were conducted on these two strains, which were identified as Fusarium solani (M15) and Aspergillus sclerotiorum (M16). This method was shown to be a feasible, accurate and convenient way to identify fungi in stored grain.

Isolation and Identification Rust Pathogens and the Study of Antioxidant Enzyme Activity and Gene Expression under Rust Infection in Zoysia japonica

“…Identification of the zoysiagrass rust pathogen. Identification of the pathogenic fungal strain is beneficial for selecting effective fungicide for disease control and serves as the foundation for epidemiological investigations [27]. Previously, Li [28] isolated and purified the pathogen from rust-infected hyacinth leaves, performed 18S rDNA sequence homology analysis, and identified the rust pathogen as Uromyces muscari.…”