Separation and purification of inulin oligomers and polymers by reversed-phase high-performance liquid chromatography

Heinze, Brian C.; Praznik, Werner

doi:10.1002/app.1991.070480017

Cited by 11 publications

(7 citation statements)

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“…The assignment of these chromatographic peaks was based on the generally accepted assumption that the retention time of a homologous series of carbohydrates increased as the DP increased. Furthermore, each FOS eluted before IOS having the same DP, according to works reported in literature 7–8. Following these assumptions, the elution order of peaks depicted in Fig.…”

Section: Resultsmentioning

confidence: 96%

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Characterization and quantitation of short‐chain fructooligosaccharides and inulooligosaccharides in fermented milks by high‐performance anion‐exchange chromatography with pulsed amperometric detection

Borromei

Cavazza

Merusi

et al. 2009

J of Separation Science

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With the increased interest in the possible link between fructooligosaccharides (FOS) intake from functional foods and health, the need for reliable data on the individual FOS content of those foods has become very important. In this study a new high-performance anion-exchange chromatography coupled to pulsed amperometric detection (HPAEC-PAD) method is described for the selective determination of short-chain FOS and inulooligosaccharides (IOS) present in the investigated prebiotic food ingredients. Response factor of PAD was investigated in relationship with degree of polymerization and isomers. The developed HPAEC-PAD method has been applied to characterize shortchain oligosaccharides of the inulin series with DP ranging from DP3 to DP7 and IOS from DP2 to DP7. Their quantification in commercial water-soluble fibers of relevant importance as prebiotic food ingredient added in fermented milks has been performed. In order to evaluate the reliability of the proposed method, results have been compared with those obtained by the official enzymatic method. Furthermore, this new HPAEC-PAD method may contribute to clarify the real importance, prebiotic effectiveness and biological role of these carbohydrates.

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Section: Resultsmentioning

confidence: 96%

“…In another work 7 it is shown that Fn and GFn molecules do not have the same response factor in the HPAEC‐PAD system. In detail, the sensitivity of the PAD decreases clearly from DP2 to DP8 8.…”

Section: Introductionmentioning

confidence: 94%

Characterization and quantitation of short‐chain fructooligosaccharides and inulooligosaccharides in fermented milks by high‐performance anion‐exchange chromatography with pulsed amperometric detection

Borromei

Cavazza

Merusi

et al. 2009

J of Separation Science

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“…Fructans were identified by comparison of their retention times with those of fructan standards isolated and purified from H . tuberosus (Sigma) according to the method of Heinze and Praznik (1991). The fructan oligomers were quantified according to the method of Timmermans et al (1994), using rhamnose as an interna1 standard.…”

Section: Analysis Of Sugars and Fructansmentioning

confidence: 99%

Purification and Characterization of the Enzymes of Fructan Biosynthesis in Tubers of Helianthus tuberosus Colombia (II. Purification of Sucrose:Sucrose 1-Fructosyltransferase and Reconstitution of Fructan Synthesis in Vitro with Purified Sucrose:Sucrose 1-Fructosyltransferase and Fructan:Fructan 1-Fructosyltransferase)

1996

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Sucrosesucrose 1 -fructosyltransferase (1 -SST), an enzyme involved in fructan biosynthesis, was purified to homogeneity from tubers of Helianfbus fuberosus that were harvested in the accumulation phase. Cel filtration under native conditions predicted a molecular mass of about 67 kD. Electrophoresis or gel filtration under denaturing conditions yielded a 27-and a 55-kD fragment. 1 -SST preferentially catalyzed the conversion of sucrose into the trisaccharide 1 -kestose (CF,). Other reactions catalyzed by 1 -SST at a lower rate were self-transfructosylations with CF, and 1,l -nystose (CF,) as substrates yielding CF, and 1,1,1 -fructosylnystose, respectively, as products. 1 -SST also catalyzed the removal of the terminal fructosyl unit from both CF, and CF,, which resulted in the release of sucrose and CF,, respectively, and free Fru. The purified enzyme did not display P-fructosidase activity. An enzyme mixture of purified 1 -SST and fructan:fructan 1 -fructosyltransferase, both isolated from tubers, was able to synthesize fructans up to a degree of polymerization of at least 13 with sucrose as a sole substrate.

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“…Sugars were detected with a differential refractometer (Waters 410) and quantified using fructose, glucose, sucrose and inulin (from Jerusalem ArtichokesSigma) as standards. Fructan oligomers were quantified individually using HPLC (Heinze & Praznik, 1991) with an rp-column (Spherisorb ODS H, Knauer-Berlin) eluted with a gradient (1-10% methanol in 60 min) at 0-8 mol min"^ and detected by Varex ELSD (Evaporative light scattering detector). Additionally fructans were quantified on a Dionex-System using the following parameters: detector.…”

Section: Plant Growthmentioning

confidence: 99%

Accumulation of fructans following oxygen deficiency stress in related plant species with different flooding tolerances

Albrecht

BIEMELT

Baumgartner³

1997

New Phytologist

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SUMMARYIn the present work we compare responses to hypoxia of the carbohydrate content of related plant species, which grow naturally on sites prone to flooding {Senecio aquaticus Hill., Myosotispalustris (L.) Lehm. Rchb.) with plants from habitats with only a low risk of oxygen shortage (Senecio jacobaea L., Myosotis arvensis (L.) Hill.). Whilst the amounts of glucose, fructose and sucrose changed only slightly following hypoxia and peaked at a maximum approximately double that of the aerated control specimens, the fructan content increased fivefold to tenfold. In nearly all the plants studied, fructan became the main polysaccharide reserve. For example, the flooding-tolerant Senecio aquaticus accumulated the highest amount, in particular fructans with a degree of pol^^merization up to 35 compared with 10 under control conditions. Nearly 70% of the soluble carbohydrates were fructans, compared with 30 % under aerated conditions.In all species tested the starch fraction marginally increased or remained constant. Fructans were found to accumulate as a response to oxygen deficiency in both flooding-tolerant and intolerant species but with higher absolute values and ratios between fructan and starch in the fiooding-tolerant species. At 24 h after the otiset of hypoxic treatment, the sugar content rose, in spite of the diminished photosynthetic rates. The ability to accumulate fructans seems to vary in plants coping with oxygen shortage but could be interpreted as advantageous in comparison to starch synthesis in terms of (i) the location of the fructan metabolism in the vacuoles, with no negative feedback in the photosynthetic apparatus and (ii) the possibility of storage of sucrose in the form of fructose polymers without the intermediate stages and energy-using processes which are indispensable for starch synthesis. These results might indicate that fructan metabolism could play a role in the tolerance of oxygen deficiency.

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Separation and purification of inulin oligomers and polymers by reversed-phase high-performance liquid chromatography

Cited by 11 publications

References 0 publications

Characterization and quantitation of short‐chain fructooligosaccharides and inulooligosaccharides in fermented milks by high‐performance anion‐exchange chromatography with pulsed amperometric detection

Characterization and quantitation of short‐chain fructooligosaccharides and inulooligosaccharides in fermented milks by high‐performance anion‐exchange chromatography with pulsed amperometric detection

Accumulation of fructans following oxygen deficiency stress in related plant species with different flooding tolerances

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