“…The activity measurements were performed according to the modifications by Prinz et al [23]. All measurements were performed in triplicates and the averaged values were calculated.…”
Section: Activity Measurement Of Laccasesmentioning
confidence: 99%
“…For this study, a system composed of PEG-3000 with an amount of 13% (w/w) and phosphate salt with 6% (w/w) related to the amount of phosphate ions (PO 4 3− ) was used, as it was previously investigated for the extractive separation of laccases from the culture supernatant of Pleurotus sapidus [23]. All necessary data about the system's phases composition and influence of NaCl on laccases activity partition coefficient is given in [23].…”
Section: Preparation Of Atpsmentioning
confidence: 99%
“…All necessary data about the system's phases composition and influence of NaCl on laccases activity partition coefficient is given in [23]. As described in the introduction, a partition coefficient around one is desired for CPC separations.…”
“…Aqueous two-phase extraction (ATPE) has been reported for successful purification of mAbs and other biological compounds [4,5,6,7,8,9,10,11,12,13].…”
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