Separation and quantitative analysis of anthraquinones in Morinda officinalis How. by HPLC

Kyoung, Kim Ho; Byungkil, C.; Mi, Chi; Yeong, Lee A; Won, Lai

doi:10.1055/s-2006-950095

Cited by 3 publications

(3 citation statements)

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“…Currently, several analytical methods based on HPLC coupled to ultraviolet detection (UV) [ 13 , 14 , 15 , 16 , 17 ] or mass spectrometry (MS) (Li et al, 2016) have been reported for the determination of anthraquinones or iridoid glycosides in M. officinalis . However, these methods suffered from low resolution or long run-time, with few of these investigations being conducted for the simultaneous determination of multicomponents in this herbal medicine [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Analysis of Iridoid Glycosides and Anthraquinones in Morinda officinalis Using UPLC-QqQ-MS/MS and UPLC-Q/TOF-MSE

2018

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Morinda officinalis is an important herbal medicine and functional food, and its main constituents include anthraquinone and iridoid glycosides. Quantification of the main compounds is a necessary step to understand the quality and therapeutic properties of M. officinalis, but this has not yet been performed based on liquid chromatography/tandem mass spectrometry (LC-MS/MS). Analytes were extracted from M. officinalis by reflux method. Ultrahigh-performance liquid chromatography coupled with a triple quadrupole mass spectrometry (UPLC-QqQ-MS) using multiple reaction monitoring (MRM) mode was applied for quantification. Fragmentation pathways of deacetyl asperulosidic acid and rubiadin were investigated based on UPLC with quadrupole time-of-flight tandem mass spectrometry (Q/TOF-MS) in the MSE centroid mode. The method showed a good linearity over a wide concentration range (R2 ≥ 0.9930). The limits of quantification of six compounds ranged from 2.6 to 27.57 ng/mL. The intra- and inter-day precisions of the investigated components exhibited an RSD within 4.5% with mean recovery rates of 95.32–99.86%. Contents of selected compounds in M. officinalis varied significantly depending on region. The fragmentation pathway of deacetyl asperulosidic and rubiadin was proposed. A selective and sensitive method was developed for determining six target compounds in M. officinalis by UPLC-MS/MS. Furthermore, the proposed method will be helpful for quality control and identification main compounds of M. officinalis.

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Section: Introductionmentioning

confidence: 99%

Simultaneous Analysis of Iridoid Glycosides and Anthraquinones in Morinda officinalis Using UPLC-QqQ-MS/MS and UPLC-Q/TOF-MSE

2018

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“…Recently, studies on M. officinalis including chemical ingredients and relevant pharmacological properties have been performed. Iridoids isolated from M. officinalis have displayed obvious antinociceptive and anti-inflammatory effects [2], oligosaccharides have been shown to possess antidepressant effects [3] and its anthraquinones have been isolated [4]. Polysaccharides have also been determined and recently were shown to have protective effect against bone loss [5,6].…”

Section: Introductionmentioning

confidence: 99%

Structural characterization and anti-fatigue activity of polysaccharides from the roots of Morinda officinalis

Zhang

et al. 2009

International Journal of Biological Macromolecules

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“…Several QC analyses of a few of the major M. officinalis anthraquinones or iridoid glycoside compounds have been carried out using HPLC coupled to a UV detector . However, there is an increasing demand for accurate and consistent measurements of the bioactivity of phytomedicine.…”

Section: Introductionmentioning

confidence: 99%

Evaluation and quantitative analysis of 11 compounds in Morinda officinalis using ultra‐high performance liquid chromatography and photodiode array detection coupled with chemometrics

Zhao

Kong

Zhou

et al. 2017

J of Separation Science

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Morinda officinalis (Rubiaceae) is a traditional Chinese medicine widely used for the treatment of impotence and osteoporosis in clinical therapy. In the present study, a rapid and simple ultra-high performance liquid chromatography with photodiode array detection method was developed and validated for the simultaneous determination of 11 bioactive compounds in M. officinalis. This assay method was validated with respect to linearity (R > 0.9991), precision, repeatability, limit of detection, limit of quantification, and accuracy (with observed recovery rates between 94.21 and 100.38%). The quantitative results revealed significant differences in the concentrations of the selected compounds. Additionally, chemometric methods, including hierarchical clustering analysis, principal component analysis, and partial least-squares discriminate analysis, were applied to compare and sort the 25 batches of M. officinalis samples based on the quantitative data of the analytes. All of the samples were clearly divided into two groups: the Hainan samples were successfully discriminated from the samples from other origins. Simultaneous determination of multiple compounds using the proposed method combined with chemometrics could be a viable strategy to compare and evaluate the quality of M. officinalis.

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Separation and quantitative analysis of anthraquinones in Morinda officinalis How. by HPLC

Cited by 3 publications

References 0 publications

Simultaneous Analysis of Iridoid Glycosides and Anthraquinones in Morinda officinalis Using UPLC-QqQ-MS/MS and UPLC-Q/TOF-MSE

Simultaneous Analysis of Iridoid Glycosides and Anthraquinones in Morinda officinalis Using UPLC-QqQ-MS/MS and UPLC-Q/TOF-MSE

Structural characterization and anti-fatigue activity of polysaccharides from the roots of Morinda officinalis

Evaluation and quantitative analysis of 11 compounds in Morinda officinalis using ultra‐high performance liquid chromatography and photodiode array detection coupled with chemometrics

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