Separation, identification and quantitative determination of free amino acids from plant extracts

Hodisan, T.; Culea, Monica; Cimpoiu, Claudia; Cot, Agata

doi:10.1016/s0731-7085(98)00094-6

Cited by 31 publications

(17 citation statements)

References 14 publications

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“…A number of different columns and elution protocols for many different compounds or compound classes have been developed and successfully applied for metabolic studies. Methods exist for small sugar analysis [15], amino acids [41], nucleotides [57,68], flavonoids [59], volatiles [6], or alkaloids [30].…”

Section: Metabolomics Approachesmentioning

confidence: 99%

Metabolite Measurements

Roessner¹,

Beckles²

2008

Plant Metabolic Networks

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Section: Metabolomics Approachesmentioning

confidence: 99%

Metabolite Measurements

Roessner¹,

Beckles²

2008

Plant Metabolic Networks

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“…Amino acids and fatty acids were first derivatizated to obtain trifluoroacetyl ester derivatives in the case of amino acids [6][7][8][9][10][11] and fatty acids methyl esters (FAMEs) for free fatty acids [12][13][14][15][16][17]. Antioxidant activities of extracts were also compared.…”

Section: Introductionmentioning

confidence: 99%

Comparative Characterization of Basil, Mint and Sage Extracts

Bleiziffer¹,

Mesaros²,

Suvar³

et al. 2017

Studia UBB Chemia

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ABSTRACT. Aromatic plants are widely used in food preparation for aroma and fragrance, but they are also a good source of amino acids and fatty acids. Three indigenous species, Basil (Ocimum basilicum), mint (Mentha piperita) and sage (Salvia officinalis) which are traditionally used in medicine and food, were characterized and compared in terms of volatile extracts, amino acids, fatty acids contents and antioxidant activities. The gas chromatographic-mass spectrometry (GC-MS) method is a suitable technique for the characterization of the compounds of the herbs extracts. Antioxidant activity was measured using extracts ability to scavenge DPPH radicals. Linalool was found the major compound in basil, menthol in mint and α-thujone in sage. The dominant amino acids identified in the plants extracts were glutamic acid and aspartic acid in basil and mint, while proline was found in high concentration in sage and mint. The total free fatty acids (TFA) were highest in mint, followed by sage and basil. The essential omega 3 alpha-linolenic acid (ALA) was identified in all three extracts. All samples exhibited antioxidant activity, sage extract having the highest antioxidant activity.

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“…Gas chromatographic -mass spectrometric (GC/MS) applications are very important: purity control, pharmacokinetic studies, metabolic studies or clinical applications for treatment and diagnosis [1][2][3][4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

“…Amino acids from plasma, wine, milk or herb samples were derivatized in two steps to obtain trifluoroacetyl ester derivatives [1,2,4]. Finally, the extracted analytes were detected by GC/MS in the electron impact (EI) mode [3][4][5][6]. The method was validated by using amino acid standard samples.…”

Section: Introductionmentioning

confidence: 99%

Amino Acids Profiles in Biological Media

Iordache¹,

Horj²,

Ani³

et al. 2010

AIP Conference Proceedings

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An accurate analytical method was developed to determine amino acids in some biological specimens by GC/MS technique. Stable isotopes provide useful tools for a variety of studies, offering ideal internal standards in quantitative information. Isotopic dilution gas chromatography -mass spectrometry (ID-GC/MS) is the techniques used for quantitative analysis of compounds labeled with stable isotopes. A Trace DSQ Thermo Finnigan quadrupole mass spectrometer coupled with a Trace GC was used. Amino acids were separated on a Rtx-5MS capillary column, 30 m x 0.25 mm, 0.25µm film thickness, using a temperature program from 50 o C, 1 min, 6 o C/min at 100 o C, 4 o C/min at 200 o C, 20 o C/min at 300 o C, (3min). The transfer line temperature was 250 o C, the injector temperature 200 o C and ion source temperature 250 o C; splitter: 10:1. Electron energy was 70eV and emission current, 100µA. The amino acids were purified on a Dowex 50W-W8 exchange resin and were derivatized in a procedure following two steps to obtain trifluoroacetyl butyl esters. The identification of amino acids was obtained by using NIST library but also by using amino acid standards.

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Separation, identification and quantitative determination of free amino acids from plant extracts

Cited by 31 publications

References 14 publications

Metabolite Measurements

Metabolite Measurements

Comparative Characterization of Basil, Mint and Sage Extracts

Amino Acids Profiles in Biological Media

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