Separation of 5‐aminovalerate from its bioconversion liquid by macroporous adsorption resin: mechanism and dynamic separation

Xu, Sheng; Lü, Xu; Li, Mei; Wang, Jing; Li, Hui; He, Xun; Jiao, Feng; Wu, Jinglan; Chen, Kequan; Ouyang, Pingkai

doi:10.1002/jctb.6249

Cited by 5 publications

(2 citation statements)

References 36 publications

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“…The adsorption effect of macroporous resin relies on the van der Waals attraction between it and the adsorbents, and work through physical adsorption through its huge specific surface. During separation, organic compounds can pass through a certain solvent according to the adsorption force and molecular weight [ 79 ]. Through the macroporous adsorption resin adsorption method, Chen et al separated the anti-tobacco mosaic virus (TMV) ε-Poly-l-lysine compounds from Streptomyces ahygroscopicus [ 80 ], and He et al separated the anti-gray mold compound tetramycin A from ZC-G-5 [ 81 ].…”

Section: Methodologies Of Screening Marine Antimicrobial Substancementioning

confidence: 99%

Screening of Marine Bioactive Antimicrobial Compounds for Plant Pathogens

Zhao

Chen

2021

Marine Drugs

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Plant diseases have been threatening food production. Controlling plant pathogens has become an important strategy to ensure food security. Although chemical control is an effective disease control strategy, its application is limited by many problems, such as environmental impact and pathogen resistance. In order to overcome these problems, it is necessary to develop more chemical reagents with new functional mechanisms. Due to their special living environment, marine organisms have produced a variety of bioactive compounds with novel structures, which have the potential to develop new fungicides. In the past two decades, screening marine bioactive compounds to inhibit plant pathogens has been a hot topic. In this review, we summarize the screening methods of marine active substances from plant pathogens, the identification of marine active substances from different sources, and the structure and antibacterial mechanism of marine active natural products. Finally, the application prospect of marine bioactive substances in plant disease control was prospected.

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Section: Methodologies Of Screening Marine Antimicrobial Substancementioning

confidence: 99%

Screening of Marine Bioactive Antimicrobial Compounds for Plant Pathogens

Zhao

Chen

2021

Marine Drugs

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“…13.4 g/L 5AVA could be successfully obtained with RaiP immobilized on a solid support ( Pukin et al, 2010 ). In addition, 5AVA could be effectively separated by macroporous adsorption resin AK-1 from bioconversion liquid with the purity of 99.3% ( Xu et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

A High-Efficiency Artificial Synthetic Pathway for 5-Aminovalerate Production From Biobased L-Lysine in Escherichia coli

Cheng

Luo

et al. 2021

Front. Bioeng. Biotechnol.

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Bioproduction of 5-aminovalerate (5AVA) from renewable feedstock can support a sustainable biorefinery process to produce bioplastics, such as nylon 5 and nylon 56. In order to achieve the biobased production of 5AVA, a 2-keto-6-aminocaproate-mediated synthetic pathway was established. Combination of L-Lysine α-oxidase from Scomber japonicus, α-ketoacid decarboxylase from Lactococcus lactis and aldehyde dehydrogenase from Escherichia coli could achieve the biosynthesis of 5AVA from biobased L-Lysine in E. coli. The H2O2 produced by L-Lysine α-oxidase was decomposed by the expression of catalase KatE. Finally, 52.24 g/L of 5AVA were obtained through fed-batch biotransformation. Moreover, homology modeling, molecular docking and molecular dynamic simulation analyses were used to identify mutation sites and propose a possible trait-improvement strategy: the expanded catalytic channel of mutant and more hydrogen bonds formed might be beneficial for the substrates stretch. In summary, we have developed a promising artificial pathway for efficient 5AVA synthesis.

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Optimization of l‐arginine purification from Corynebacterium crenatum fermentation broth

Qiao

Huang

Long

et al. 2020

J of Separation Science

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Abstractl‐Arginine has many special physiological and biochemical functions, with wide applications in the food and pharmaceutical industry. Few studies on the purification of l‐arginine from fermentation broth have been conducted; however, none of them were systematic enough for industrial scale‐up. Therefore, it is necessary to develop a highly efficient and systematic process for the purification of l‐arginine from fermentation broth. In this study, we screened out a cation exchange resin, D155, having high exchange capacity, high selectivity, and easy elution capacity, and analyzed its adsorption isotherm, thermodynamics, and kinetics using different models. Further, the process parameters of fixed‐bed ion exchange adsorption and elution were optimized, and the penetration curve during the operation was modeled. Based on the fixed‐bed ion‐exchange parameters, a 30‐column continuous ion‐exchange system was designed, and the flow velocity in each zone was optimized. Finally, to obtain a high purity of l‐arginine, the purification tests were conducted using anion exchange resin 711, and an l‐arginine yield of 99.1% and purity of 98.5% was obtained. This effective and economical method also provides a promising strategy for separation of other amino acids from the fermentation broth, which is of great significance to the l‐arginine fermentation industry.

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Separation of 5‐aminovalerate from its bioconversion liquid by macroporous adsorption resin: mechanism and dynamic separation

Cited by 5 publications

References 36 publications

Screening of Marine Bioactive Antimicrobial Compounds for Plant Pathogens

Screening of Marine Bioactive Antimicrobial Compounds for Plant Pathogens

A High-Efficiency Artificial Synthetic Pathway for 5-Aminovalerate Production From Biobased L-Lysine in Escherichia coli

Optimization of l‐arginine purification from Corynebacterium crenatum fermentation broth

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