Separation of amino acids and amines by capillary electrophoresis using poly(ethylene oxide) solution containing cetyltrimethylammonium bromide

Chen, Chuan-Kuo; Liu, Kung-Tien; Chiu, Tai-Chia

doi:10.1016/j.chroma.2009.02.054

Cited by 13 publications

(4 citation statements)

References 36 publications

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“…The use of organic solvents such as methanol and acetonitrile to improve separation has been described previously . It shows that electro‐osmotic velocity is reduced in CTAB/methanol solutions, and the migration time window is extended.…”

Section: Resultsmentioning

confidence: 99%

Determination of seven preservatives in cosmetic products by micellar electrokinetic chromatography

Huang

Chiu

2013

Intern J of Cosmetic Sci

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A micellar electrokinetic chromatography method using cetyltrimethylammonium bromide (CTAB) as a cationic surfactant, coupled with UV-Vis detection, was developed for the simultaneous determination of seven preservatives, including methyl-, ethyl-, propyl- and butyl-paraben and phenol, phenoxyethanol and resorcinol. The method involved optimizing the pH of the phosphate buffer and concentrations of CTAB, ethanol and 2-hydroxypropyl-β-cyclodextrin (HP-β-CD). The preservatives were well separated using optimum conditions and separated within 10 min at a separation voltage of -12.5 kV with the 1.0 mM phosphate buffer (pH 7.0) containing 90 mM CTAB, 25 mM HP-β-CD and 10% (v/v) ethanol. Satisfactory recoveries (84.1-103.0%), migration time (RSD < 3.1%) and peak area (RSD < 4.5%) repeatabilities were achieved. Detection limits of the preservatives were between 0.31 and 1.52 μg mL(-1) (S/N = 3, n = 5). The optimized method was successfully applied to the simultaneous determination of these preservatives in 10 commercial cosmetic products.

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Section: Resultsmentioning

confidence: 99%

Determination of seven preservatives in cosmetic products by micellar electrokinetic chromatography

Huang

Chiu

2013

Intern J of Cosmetic Sci

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“…A comparable sensitivity is reported in the analysis of naphthalene‐2,3‐dicarboxaldehyde (NDA)‐labeled AAs and amines using a LED emitting at 405 nm (maximum output at 405 nm in the range 390–420 nm), with LODs range from 9.5 to 50.5 nM, using poly(ethylene oxide) (PEO) containing CTAB. In the presence of CTAB and ACN, adsorption of PEO on the capillary wall is suppressed, leading to generation of a faster and more reproducible EOF, and the separation is obtained in less than 4 min 11. If a UV laser is used (rather than an LED) with native detection nanomolar LODs are obtained 12, using fiber‐optic LED‐induced fluorescence and CE with the LOD of D / L ‐Tyr derivatives around 2 μM 13.…”

Section: New Ce Methodsmentioning

confidence: 99%

Recent advances in amino acid analysis by CE

Poinsot

Gavard

Feurer³

et al. 2009

Electrophoresis

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This article describes the most important articles that have been published on amino acid analysis using CE during the period from June 2007 to May 2009. It follows the format of the previous articles of Smith [Electrophoresis 1999, 20, 3078-3083], Prata et al. [Electrophoresis 2001, 22, 4129-4138] and Poinsot et al. [Electrophoresis 2003, 24, 4047-4062; Electrophoresis 2006, 27, 176-194; Electrophoresis 2008, 29, 207-223]. For several years we have presented the new developments in amino acid analysis with CE which describe the use of laser emitting diodes for LIF as well as via MS. In addition, we describe articles concerning clinical studies and neuroclinical applications.

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“…PEO acts as concentration medium, and AA‐CBIs that migrate toward the PEO zone are stacked at the boundary. This principle was applied for the analysis of AAs in cerebrospinal fluid [152, 180], red wine [181], tea leaves, and beverages [182], tobacco extracts and urine [188], beer samples [183], breast cancer cells [184], and ascites (Val, Leu, Ile) [185] as well as in the chiral analysis of Asp [187] and branched‐chain AAs [186]. Fluorescence signal enhancement by the addition of α‐, β‐, HP‐β‐, and γ‐CDs in the BGE was investigated by Church et al.…”

Section: Naphthalene‐23‐dicarboxaldehydementioning

confidence: 99%

Homocyclic o‐dicarboxaldehydes: Derivatization reagents for sensitive analysis of amino acids and related compounds by capillary and microchip electrophoresis

Celá

Glatz

2020

Electrophoresis

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Amino acids are essential compounds for living organisms, and their determination in biological fluids is crucial for the clinical analysis and diagnosis of many diseases. However, the detection of most amino acids is hindered by the lack of a strong chromophore/fluorophore or electrochemically active group in their chemical structures. The highly sensitive determination of amino acids often requires derivatization. Capillary electrophoresis is a separation technique with excellent characteristics for the analysis of amino acids in biological fluids. Moreover, it offers the possibility of precapillary, on‐capillary, or postcapillary derivatization. Each derivatization approach has specific demands in terms of the chemistry involved in the derivatization, which is discussed in this review. The family of homocyclic o‐dicarboxaldehyde compounds, namely o‐phthalaldehyde, naphthalene‐2,3‐dicarboxaldehyde, and anthracene‐2,3‐dicarboxaldehyde, are powerful derivatization reagents for the determination of amino acids and related compounds. In the presence of suitable nucleophiles they react with the primary amino group to form both fluorescent and electroactive derivatives. Moreover, the reaction rate enables all of the derivatization approaches mentioned above. This review focuses on articles that deal with using these reagents for the derivatization of amino acids and related compounds for ultraviolet‐visible spectrometry, fluorescence, or electrochemical detection. Applications in capillary and microchip electrophoresis are summarized and discussed.

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Separation of amino acids and amines by capillary electrophoresis using poly(ethylene oxide) solution containing cetyltrimethylammonium bromide

Cited by 13 publications

References 36 publications

Determination of seven preservatives in cosmetic products by micellar electrokinetic chromatography

Determination of seven preservatives in cosmetic products by micellar electrokinetic chromatography

Recent advances in amino acid analysis by CE

Homocyclic o‐dicarboxaldehydes: Derivatization reagents for sensitive analysis of amino acids and related compounds by capillary and microchip electrophoresis

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