Separation of aminophenol isomers in polyelectrolyte multilayers modified PDMS microchip

Yan, Xilong; Wang, Kai; Yu, Xiao-Dong; Xu, Jing‐Juan; Chen, Hong‐Yuan

doi:10.1016/j.talanta.2007.01.037

Cited by 24 publications

(19 citation statements)

References 26 publications

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“…Successive multiple ionic layer (SMIL) (also referred to as polyelectrolyte multilayer) coatings have been introduced as semi-permanent coatings for conventional [16,[27][28][29] and MCE [25,[30][31][32]. SMIL coatings are formed by the deposition of alternating layers of polyelectrolytes on a surface as first reported by Decher et al [33,34].…”

Section: Introductionmentioning

confidence: 99%

Influence of polymer structure on electroosmotic flow and separation efficiency in successive multiple ionic layer coatings for microchip electrophoresis

et al. 2008

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The effect of successive multiple ionic layer (SMIL) coatings on the velocity and direction of EOF and the separation efficiency for PDMS electrophoresis microchips was studied using different polymer structures and deposition conditions. To date, the majority of SMIL studies have used traditional CE and fused-silica capillaries. EOF was measured as a function of polymer structure and number of layers, in one case using the same anionic polymer and varying the cationic polymer and in the second case using the same cationic polymer and varying the anionic polymer. In both situations, the EOF direction reversed with each additional deposited polymer layer. The absolute EOF magnitude, however, did not vary significantly with layer number or polymer structure. Next, different coatings were used to compare separation efficiencies on native and SMIL-coated PDMS microchips. For native PDMS microchips, the average separation efficiency was 4105 +/- 1540 theoretical plates. The addition of two layers of polymer increased the separation efficiency anywhere from two- to five-fold, depending on the polymer structure. A maximum separation efficiency of 12 880 +/- 1050 theoretical plates was achieved for SMIL coatings of polybrene (cationic) and dextran sulfate (anionic) polymers after deposition of six total layers. It was also noted that coating improved run-to-run consistency of the peaks as noted by a reduction of the RSD of the EOF and separation efficiency. This study shows that the use of polyelectrolyte coatings, irrespective of the polymer structure, generates a consistent EOF in the current experiments and dramatically improves the separation efficiency when compared to unmodified PDMS microchips.

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Section: Introductionmentioning

confidence: 99%

Influence of polymer structure on electroosmotic flow and separation efficiency in successive multiple ionic layer coatings for microchip electrophoresis

et al. 2008

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“…A bilayer of (PDDA-PSS) 2 modified PDMS microchip 26 is always reported as an efficient measure for improvement of capillary separation. The separation of the four analysts was also carried out in a (PDDA-PSS) 2 modified PDMS microchip (Fig.…”

Section: Electrophoretic Separation Efficiency On the Coated Channelmentioning

confidence: 99%

“…Xiao et al 26 used alternating layers of poly(dimethylammoniumchloride) (PDDA) and PSS to form a (PDDA/PSS) 2 modified PDMS microchannel, which achieved 100 multiples improvement of separation efficiency. Qiu et al 27 also reported a similar (PDDA/PSS) 2 modified PDMS microchannel to obtain a stable EOF due to reduced non-specific adsorption.…”

Section: Introductionmentioning

confidence: 99%

Electrophoretic separation of neurotransmitters on a polystyrene nano-sphere/polystyrene sulphonate coated poly(dimethylsiloxane) microchannel

Zhao¹,

Zhang²,

Yang³

et al. 2011

Biomicrofluidics

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In this paper, a poly(dimethylsiloxane) microchip with amperometric detector was developed for the electrophoretic separation and determination of neurotransmitters. For increasing the separation efficiency, the microchannel is modified by polystyrene sulphonate/polystyrene nano-sphere self-assembly coating. A stable electro-osmotic flow (EOF) and higher separation efficiency are obtained in proposed modified microchannel. Under optimized conditions, dopamine, epinephrine, catechol, and serotonin are acceptably baseline separated in this 3.5 cm length separation channel with the theoretical plate number from 4.6 × 104 to 2.1 × 105 per meter and resolution from 1.29 to 12.5. The practicability of proposed microchip is validated by the recovery test with cerebrospinal fluid as real sample which resulted from 91.7% to 106.5%.

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“…In addition, many separation and detection systems incorporating microchips have the potential to effectively identify and quantify environmental pollutants (Veuthey 2005;Dabek-Zlotorzynska et al 2008;Yang et al 2001;Li and Lin 2009;Xiao et al 2007;Holland and Leigh 2002;Stege et al 2009). Of the methods available, microchip capillary electrophoresis-electrochemical detection (CE-ECD) has the advantages of low cost, short analysis time, minimal wastewater output, high separation efficiency and high sensitivity, in addition to the potential for miniaturization (Song and Chen 2001;Chen et al 2006Wang 2005).…”

Section: Introductionmentioning

confidence: 99%

Enhanced aminophenols monitoring using in-channel amperometric detection with dual-channel microchip capillary electrophoresis

Hahn

2010

Environ Chem Lett

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Improved techniques are needed to monitor toxic aminophenols in industrial wastewater. Microchip capillary electrophoresis (CE)-electrochemical detection (ECD) can be used to develop miniaturized system providing on-site monitoring. Here, a dual-channel microchip CE-ECD has been applied for the monitoring of aminophenols. The effect of conductance and capacitance between a working electrode and a reference electrode has been studied to minimize the noise that was generated in the amperometric detection. A mixture of aminophenols dissolved in a running buffer has been monitored. A separation efficiency with a plate number of 4770 and a high linear concentration dependence from 100 nM to 1 mM have been achieved for p-aminophenol. As a result, the analysis system has been applied for the monitoring of the pollutants with low detection limits of p-aminophenol (14.6 nM), o-aminophenol (10.6 nM) and m-aminophenol (15.3 nM). Here, we show that a wastewater sample from a natural gas refinery can be monitored using the microchip CE-ECD system.

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Separation of aminophenol isomers in polyelectrolyte multilayers modified PDMS microchip

Cited by 24 publications

References 26 publications

Influence of polymer structure on electroosmotic flow and separation efficiency in successive multiple ionic layer coatings for microchip electrophoresis

Influence of polymer structure on electroosmotic flow and separation efficiency in successive multiple ionic layer coatings for microchip electrophoresis

Electrophoretic separation of neurotransmitters on a polystyrene nano-sphere/polystyrene sulphonate coated poly(dimethylsiloxane) microchannel

Enhanced aminophenols monitoring using in-channel amperometric detection with dual-channel microchip capillary electrophoresis

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