Electrophoretic separation of neurotransmitters on a polystyrene nano-sphere/polystyrene sulphonate coated poly(dimethylsiloxane) microchannel

Abstract

In this paper, a poly(dimethylsiloxane) microchip with amperometric detector was developed for the electrophoretic separation and determination of neurotransmitters. For increasing the separation efficiency, the microchannel is modified by polystyrene sulphonate/polystyrene nano-sphere self-assembly coating. A stable electro-osmotic flow (EOF) and higher separation efficiency are obtained in proposed modified microchannel. Under optimized conditions, dopamine, epinephrine, catechol, and serotonin are acceptably baseline separated in this 3.5 cm length separation channel with the theoretical plate number from 4.6 × 104 to 2.1 × 105 per meter and resolution from 1.29 to 12.5. The practicability of proposed microchip is validated by the recovery test with cerebrospinal fluid as real sample which resulted from 91.7% to 106.5%.

Electrophoretic Separation of Chlorophenols on Silica Nanospheres-coated Poly(dimethylsiloxane) Microchip Using a Nafion/graphene-modified Carbon Electrode for Detection

“…2, the EOF is more stable against any pH alteration on a modified microchannel other than on a native one. By comparing of the alteration of the EOF values, depending on the pH with reported similar methods [20][21][22][23] ( Table 1), we could find that there was only 0.06 of ΔEOF during a pH change from 8.0 to 11.0, which is the smallest one. Noticeably, there are at least one of two main aspects of difference between our research and those reports.…”

“…et al 20 successfully separated the neurotransmitters using a polystyrene nanosphere modified PDMS chip. Qiu et al 21 reported a poly(diallyldimethylammonium chloride) (PDDA)/poly(sodium-p-styrenesulfonate) (PSS) modified PDMS chip to obtain a stable EOF based on reduced non-specific adsorption.…”

Recent Trends in the Quantification of Biogenic Amines in Biofluids as Biomarkers of Various Disorders: A Review

“…Compared with the classical CE method previously reported, the method described by Tang et al [102] showed approximately a 100-fold enhancement, which allowed μM levels of LODs to be obtained for the analytes. Zhao et al developed another microchip electrophoresis (MCE) application with AmpD (Ag/AgCl) to determine DA, E, 5-HT and catechol in spiked CSF from healthy human samples using a microfluidic poly-dimethylsiloxane (PDMS) device with its microchannel coated with polystyrene nanosphere/polystyrene sulfonate to increase the separation efficiency and to stabilize the EOF [103].…”

Development of a Plastic-Based Microfluidic Immunosensor Chip for Detection of H1N1 Influenza

“…After the development of soft lithography techniques using poly(dimethylsiloxane) (PDMS), PDMS has become the most popular microfluidic device materials and offers several advantages such as easy handling, good sealing properties and high optical transparency [ 6 ]. However, the poor chemical stability in different types of organic solvents, difficulty in surface modification and mass production have limited the use of PDMS in the various applications [ 7 ].…”