Separation of anaesthetic ketamine and its derivates in PAMAPTAC coated capillaries with tuneable counter-current electroosmotic flow

Tůma, Petr; Koval, Dušan; Sommerová, Blanka; Vaculín, Šimon

doi:10.1016/j.talanta.2020.121094

Cited by 22 publications

(18 citation statements)

References 39 publications

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“…The speed of the analyte in the capillary is proportional to the difference between the electrophoretic mobility and the EOF mobility, resulting in a slow rate of migration within the capillary. The elec-trophoretic separation time is long, contributing to mutual separation of the analytes with similar mobility values, as was demonstrated in our previous paper [50].…”

Section: Electrophoretic Stacking With Whole Capillary Sample Injectionsupporting

confidence: 65%

“…Contrary, the forcing out of the matrix by an external pressure is accompanied by sample losses, as was demonstrated in our previous work [49]. With PAMAPTAC coating, the magnitude of the EOF is controlled by the content of cationic APTAC components in the polymerisation mixture, where 6% PAMAPTAC coating was found suitable for the separation of methylarginines [50].…”

Section: Electrophoretic Stacking With Whole Capillary Sample Injectionmentioning

confidence: 67%

“…The capillary parameters are a 25 µm inner diameter (ID), 360 µm outer diameter (OD), total length of 39.45 cm and length to C 4 D 25.05 cm. The PAMAPTAC capillary was prepared in the laboratory according the procedure in [53,54] and described in detail in our recent publications [49,50]. The 6% PAMAPTAC capillary was characterised by an anodic EOF mobility with a value of (−17.3 ± 0.0) 10 −9 m 2 V −1 s −1 in 750 mM AcOH as background electrolyte (BGE).…”

Section: Pamaptac-coated Capillarymentioning

confidence: 99%

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Electrophoretic Determination of Symmetric and Asymmetric Dimethylarginine in Human Blood Plasma with Whole Capillary Sample Injection

Tůma

Sommerová

Koval

et al. 2021

IJMS

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Asymmetric and symmetric dimethylarginines are toxic non-coded amino acids. They are formed by post-translational modifications and play multifunctional roles in some human diseases. Their determination in human blood plasma is performed using capillary electrophoresis with contactless conductivity detection. The separations are performed in a capillary covered with covalently bonded PAMAPTAC polymer, which generates anionic electroosmotic flow and the separation takes place in the counter-current regime. The background electrolyte is a 750 mM aqueous solution of acetic acid with pH 2.45. The plasma samples for analysis are treated by the addition of acetonitrile and injected into the capillary in a large volume, reaching 94.5% of the total volume of the capillary, and subsequently subjected to electrophoretic stacking. The attained LODs are 16 nm for ADMA and 22 nM for SDMA. The electrophoretic resolution of both isomers has a value of 5.3. The developed method is sufficiently sensitive for the determination of plasmatic levels of ADMA and SDMA. The determination does not require derivatization and the individual steps in the electrophoretic stacking are fully automated. The determined plasmatic levels for healthy individuals vary in the range 0.36–0.62 µM for ADMA and 0.32–0.70 µM for SDMA.

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Section: Electrophoretic Stacking With Whole Capillary Sample Injectionsupporting

confidence: 65%

Section: Electrophoretic Stacking With Whole Capillary Sample Injectionmentioning

confidence: 67%

Section: Pamaptac-coated Capillarymentioning

confidence: 99%

See 1 more Smart Citation

Electrophoretic Determination of Symmetric and Asymmetric Dimethylarginine in Human Blood Plasma with Whole Capillary Sample Injection

Tůma

Sommerová

Koval

et al. 2021

IJMS

Self Cite

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“…or intravenous (i.v.) ketamine injection in rodents, dogs, and horses (Leung and Baillie, 1986;Moaddel et al, 2015;Pham et al, 2018;Tuma et al, 2020;Yamaguchi et al, 2018;Zanos et al, 2016Zanos et al, , 2019a, and at the end of a 40-min i.v. infusion in humans (Farmer et al, 2020;Kurzweil et al, 2020;Zarate et al, 2012b;Zhao et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Sex-dependent metabolism of ketamine and (2R,6R)-hydroxynorketamine in mice and humans

et al. 2021

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Background: Ketamine is rapidly metabolized to norketamine and hydroxynorketamine (HNK) metabolites. In female mice, when compared to males, higher levels of ( 2R,6R;2S,6S)-HNK have been observed following ketamine treatment, and higher levels of ( 2R,6R)-HNK following the direct administration of ( 2R,6R)-HNK. Aim: The objective of this study was to evaluate the impact of sex in humans and mice, and gonadal hormones in mice on the metabolism of ketamine to form norketamine and HNKs and in the metabolism/elimination of ( 2R,6R)-HNK. Methods: In CD-1 mice, we utilized gonadectomy to evaluate the role of circulating gonadal hormones in mediating sex-dependent differences in ketamine and ( 2R,6R)-HNK metabolism. In humans (34 with treatment-resistant depression and 23 healthy controls) receiving an antidepressant dose of ketamine (0.5 mg/kg i.v. infusion over 40 min), we evaluated plasma levels of ketamine, norketamine, and HNKs. Results: In humans, plasma levels of ketamine and norketamine were higher in males than females, while ( 2R,6R;2S,6S)-HNK levels were not different. Following ketamine administration to mice (10 mg/kg i.p.), Cmax and total plasma concentrations of ketamine and norketamine were higher, and those of ( 2R,6R;2S,6S)-HNK were lower, in intact males compared to females. Direct ( 2R,6R)-HNK administration (10 mg/kg i.p.) resulted in higher levels of ( 2R,6R)-HNK in female mice. Ovariectomy did not alter ketamine metabolism in female mice, whereas orchidectomy recapitulated female pharmacokinetic differences in male mice, which was reversed with testosterone replacement. Conclusion: Sex is an important biological variable that influences the metabolism of ketamine and the HNKs, which may contribute to sex differences in therapeutic antidepressant efficacy or side effects.

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“…PAMAPTAC coating generates anionic EOF and faster AAs cations migrate in the opposite direction to EOF in counter current electrophoretic mode. This approach improves the electrophoretic resolution of neighboring peaks, but at the same time prolongs the separation time by approximately twofold and deteriorates the separation efficiency [39].…”

Section: Resultsmentioning

confidence: 99%

Monitoring of circulating amino acids in patients with pancreatic cancer and cancer cachexia using capillary electrophoresis and contactless conductivity detection

et al. 2021

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Branched chain amino acids (BCAAs), alanine and glutamine are determined in human plasma by capillary electrophoresis with contactless conductivity detection (CE/C4D). The baseline separation of five amino acids from other plasma components is achieved on the short capillary effective length of 18 cm in 3.2 mol/L acetic acid with addition of 13% v/v methanol as background electrolyte. Migration times range from 2.01 min for valine to 2.84 min for glutamine, and LODs for untreated plasma are in the interval 0.7–0.9 μmol/L. Sample treatment is based on the addition of acetonitrile to only 15 μL of plasma and supernatant is directly subjected to CE/C4D. Circulating amino acids are measured in patients with pancreatic cancer and cancer cachexia during oral glucose tolerance test. It is shown that patients with pancreatic cancer and cancer cachexia syndrome exhibit low basal circulating BCAAs and glutamine levels and loss of their insulin‐dependent suppression.

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Separation of anaesthetic ketamine and its derivates in PAMAPTAC coated capillaries with tuneable counter-current electroosmotic flow

Cited by 22 publications

References 39 publications

Electrophoretic Determination of Symmetric and Asymmetric Dimethylarginine in Human Blood Plasma with Whole Capillary Sample Injection

Electrophoretic Determination of Symmetric and Asymmetric Dimethylarginine in Human Blood Plasma with Whole Capillary Sample Injection

Sex-dependent metabolism of ketamine and (2R,6R)-hydroxynorketamine in mice and humans

Monitoring of circulating amino acids in patients with pancreatic cancer and cancer cachexia using capillary electrophoresis and contactless conductivity detection

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