Separation of antidepressants by capillary electrophoresis with in-line solid-phase extraction using a novel monolithic adsorbent

Schaller, David; Hilder, Emily F.; Haddad, Paul R.

doi:10.1016/j.aca.2005.07.035

Cited by 69 publications

(48 citation statements)

References 47 publications

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“…In capillaries, there are a number of reports of using a monolith for in-line extraction. Baryla et al [260] used a methacrylic acid monolith for the extraction of propanol, and Schaller et al [261] and Breadmore [262] have used a sulphopropylmethacrylate monolith for the extraction of antidepressants and amino acids, respectively. In all the cases, an increase in sensitivity of several orders of magnitude was obtained.…”

Section: Spementioning

confidence: 99%

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips

2007

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Poor sensitivity is considered to be one of the major limitations of electrophoretic separation methods, particularly when compared to traditional liquid chromatographic techniques. To address this issue, various in-line preconcentration techniques have been developed over the past 15 years, ranging in power and complexity, and there are now a number of well understood approaches routinely capable of providing a 10,000- to 100,000-fold increase in sensitivity, as well as several that can be pushed above a million. Furthermore, these have been achieved with particularly troublesome and often difficult samples, such as those having high salinity from a biological or environmental origin. This review will discuss the most common methods for improving the sensitivity of CE, CEC and microchip version of these, with particular attention to those approaches developed over the last five years.

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Section: Spementioning

confidence: 99%

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips

2007

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“…Ethamsylate, tramadol, and lidocaine are partially excreted by kidneys, and are generally used as hemostatic, analgesic, and local anesthetic in surgery. Antihistamines [127], antidepressants [128], morphine and related compounds [127], protease inhibitors [129], antibiotics [40,133,134,138,139], and amino acids [140,141] are a few examples of drugs that have been detected by CE.…”

Section: Ce In Pharmaceuticsmentioning

confidence: 99%

CE at the omics level: Towards systems biology – An update

Song

Babar

et al. 2007

Electrophoresis

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This review provides an updated overview of recent developments and applications of CE based on previously published reports in the field of omic research. The increased number of published articles on omics shows that the field is growing and attracting the attention of many life science researchers. Due to developments in the omics sciences, many researchers have been studying systems biology, in which biological events in organisms are systematically interpreted through the combination of complex measurements from various methods resulting in high-throughput data. Given the challenges of such complex forms of analysis, CE is a strong candidate for generating omics data useful for acquiring the qualitative and quantitative knowledge necessary for systems-level investigation. By emphasizing CE for systems biology, this review will discuss and focus on the applicability of CE to systems-based analytical data at the genomic, transcriptomic, proteomic, and metabolomic levels from 2005 to the present.

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“…A LOD in the low-nM range was reported after the analysis of this single compound. To demonstrate the usefulness of in-line monolithic SPE-CE to the simultaneous preconcentration and separation of a mixture of analytes, Schaller et al [17] prepared a negatively charged methacrylate polymer monolith from 3-sulfopropyl methacrylate-cobutyl methacrylate-co-ethylene dimethacrylate. Using a 1-cm SPE module at the inlet end of the capillary, a mixture of sertraline, fluoxetine and fluvoxamine was preconcentrated and followed by baseline separation by CE.…”

Section: Introductionmentioning

confidence: 99%

In‐line coupling SPE and CE for DNA preconcentration and separation

et al. 2011

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An in-line SPE method coupled to CE was developed for the analysis of DNA. The amino silica monolith was prepared in situ by polymerization of tetraethoxysilane and N-(β-aminoethyl)-γ-aminopropyltriethoxysilane in ethanol aqueous solution at the inlet end of a 100 μm id fused-silica capillary, and the remaining part of the capillary was used as separation channel. The procedure for this in-line SPE-CE method was constructed on the basis of investigation on operational conditions such as the introduction mode of sieving matrix, the composition of elution solvent and the elution time. Twenty millimolar ammonium hydroxide was demonstrated to be effective for DNA desorption from the monolith, and linear poly(N-isopropylacrylamide) was used as the separation matrix. The proposed method could achieve limits of detection of 0.065-0.123 ng/mL for six DNA fragments ranging 100-2000 bp. Compared with conventional CE, preconcentration factors of over 100 times were obtained. The applicability of the in-line SPE-CE method was further demonstrated by analyzing plasmid DNA from Escherichia coli crude lysate.

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Separation of antidepressants by capillary electrophoresis with in-line solid-phase extraction using a novel monolithic adsorbent

Cited by 69 publications

References 47 publications

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips

CE at the omics level: Towards systems biology – An update

In‐line coupling SPE and CE for DNA preconcentration and separation

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