Separation of Chlordiazepoxide and Selected Chlordiazepoxide Mixtures Using Capillary SFC

Abstract

Journal of Liquid ChromatographyPublication details, including instructions for authors and subscription information: ABSTRACTThe analysis of chlordiazepoxide and selected chlordiazepoxide mixtures using supercritical fluid chromatography (SFC) has been investigated. The separations were carried out on four different stationary phases (SBmethyl-I 00, SB-biphenyl-30, SB-smectic and SB-cyanopropyl-50) with carbon dioxide as the mobile phase and flame ionization detection. The relative retention data obtained using the SFC method is compared with HPLC methods.

Supercritical Fluid Chromatography and Extraction

“…Peytavin et al reviewed the potential of open-tubular SFC for the determination of polar pharmaceutical actives (244). Jagota and Stewart published a number of evaluations of open-tubular SFC for the determination of particular types of actives: estrogens (245), diazepam, chlordiazepoxide, and related compounds (246,247), and nonsteroidal antiinflammatory agents (248). Of three polysiloxane columns evaluated (100% methyl, 30% biphenyl/70% methyl, and 50% cyanopropyl/50% methyl), the cyanopropyl provided best results for the estrogens and the diazepam-and chlordiazepoxide-related compounds, while greater success was achieved with the biphenyl column for the antiinflammatory compounds.…”

Forensic Toxicology; Current Status/Recent Trends

“…By means of modifiers added to the gas, one can effectively program the chromatography without the high temperatures of GC or the solvent use of HPLC (53)(54)(55)(56)(57)(58).…”

Simultaneous Determination of Clidinium Bromide and Chlordiazepoxide in Combined Dosage Forms by High-Performance Liquid Chromatography

“…The United States Pharmacopeia (USP) stated the nonaqueous titration method for the assay of clidinium bromide and chlordiazepoxide [ 4 ]. Few methods for the determination of clidinium bromide and chlordiazepoxide in combined dosage forms including HPLC [ 5 – 7 ], derivative spectrophotometry [ 8 , 9 ], spectrophotometry using multivariate calibration techniques [ 10 ], and capillary SFC [ 11 ] have been reported. Literature survey revealed that some analytical methods have been used for the individual estimation of clidinium bromide and chlordiazepoxide.…”