Separation of complex mixtures of fluorobenzoic acids by capillary electrophoresis

Isemura, Tsuguhide; Kitagawa, Fumihiko; Otsuka, Koji

doi:10.1002/jssc.200800549

Cited by 9 publications

(4 citation statements)

References 22 publications

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“…A similar study was performed on a (PB/DS) 1.5 coating in phosphate buffer (without maintaining ionic strength constant) for the analysis of 25 different acids. [30] Better separation efficiency was obtained at pH 3.1 (24 peaks were observed among the 25 acids analyzed). EOF remained stable at higher pH until 10, showing good robustness for this coating.…”

Section: Impact Of the Bgementioning

confidence: 95%

“…Likewise, Isemura et al examined the stability of their optimized (PB/DS) 1.5 coating for the analysis of three fluorobenzoic acids (tetrafluorophthalic acid, 2,4,5-trifluorobenzoic acid, and 3,4-difluorobenzoic acid). [30] Figure 6 shows the evolution of EOF and the acids electrophoretic mobilities over 60 runs, the BGE being changed every 15 runs. It can be seen that the EOF was stable for 30 runs and then gradually decreased, perhaps because of a minor detachment of the final PB layer.…”

Section: Chemical Stabilitymentioning

confidence: 99%

“…CE is an efficient tool to analyze therapeutic compounds, as is shown by Isemura et al for the analysis of drug intermediates. [30] Indeed, fluorobenzoic acids (FBAs) are important intermediates in antibacterial drug synthesis and require reproducible and efficient separation which can be provided by CE. 25 derivatives were analyzed with phosphate buffers (pH 2.5-7) and a (PB/DS) 1.5 coated capillary.…”

Section: Limiting Solute Adsorption In Czementioning

confidence: 99%

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Polyelectrolyte Multilayers in Capillary Electrophoresis

et al. 2022

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Capillary electrophoresis (CE) has been proven to be a performant analytical method to analyze both small and macro molecules. Indeed, it is capable of separating compounds of the same nature according to differences in their charge to size ratios, particularly proteins, monoclonal antibodies and peptides. However, one of the major obstacles to reach high separation efficiency remains the adsorption of solutes on the capillary wall. Among the different coating approaches used to control and minimize solute adsorption, polyelectrolyte multi-layers can be applied to CE as a versatile approach. These coatings are made up of alternating layers of polycations and polyanions, and may be used in acidic, neutral or basic conditions depending on the solutes to be analyzed. This Review provides an overview of Successive Multiple Ionicpolymer Layer (SMIL) coatings used in CE, looking at how different parameters induce variations on the electro-osmotic flow (EOF), separation efficiency and coating stability, as well as their promising applications in the biopharmaceutical field.

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Section: Impact Of the Bgementioning

confidence: 95%

Section: Chemical Stabilitymentioning

confidence: 99%

Section: Limiting Solute Adsorption In Czementioning

confidence: 99%

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Polyelectrolyte Multilayers in Capillary Electrophoresis

et al. 2022

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“…Hence, there is a need for their sensitive analysis in oil reservoir waters, known for their highly dissolved organic matter and salt (NaCl, CaCl 2 ) content often exceeding 25% [3]. Gas chromatography (GC) [4] or high-performance liquid chromatography (HPLC) [5–10] with MS/MS detection have been typically used to assure the separation of the tracer compounds from each other while assuring their specific detection.…”

Section: Introductionmentioning

confidence: 99%

Rapid ion-exchange matrix removal for a decrease of detection limits in the analysis of salt-rich reservoir waters for fluorobenzoic acids by liquid chromatography coupled with tandem mass spectrometry

et al. 2016

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A matrix removal procedure with ion-exchange resin prior to analysis for 18 fluorinated benzoic acids (FBAs) tracers in saline (>25% salt) reservoir water was optimized. The elimination of >98% of salt and the simultaneous matrix sample cleanup allowed the direct analysis using the supernatant by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). This resulted in a gain in detection limits for most of the tracers in comparison with the reference method (direct analysis after minimum required dilution). The limits of detection (LODs) were in the range of 0.01–0.15 ng/ml and compared to other studies the developed method provided comparable limits of detection and advantage of simplified and shorter sample preparation. The presented method offers a considerable gain in simplicity and analysis time. Recoveries for all the tracers reached 80–100%, except for 2-FBA and 2,6-dFBA for which they were ca. 60%. The low recoveries were corrected by the use of five isotopically labeled internal standards. The method was validated by the analysis of spiked samples and by an independent comparison of the results with those obtained by solid-phase extraction LC-MS/MS method.Electronic supplementary materialThe online version of this article (doi:10.1007/s00216-016-0060-5) contains supplementary material, which is available to authorized users.

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Contribution of CE to the Analysis of Protein or Peptide Biomarkers

Mesbah

Verpillot

l’Escaille³

et al. 2013

Capillary Electrophoresis of Biomolecules

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Biomarker analysis is pivotal for disease diagnosis and one important class of biomarkers is constituted by proteins and peptides. This review focuses on protein and peptide analyses from biological fluids performed by capillary electrophoresis. The various strategies that have been reported to prevent difficulties due to the handling of real samples are described. Innovative techniques to overcome the complexity of the sample, to prevent the adsorption of the analytes on the inner capillary wall, and to increase the sensibility of the analysis are summarized and illustrated by different applications. To fully illustrate the contribution of CE to the analysis of biomarkers from human sample, two detailed protocols are given: the analysis from CSF of five amyloid peptide, biomarkers of the Alzheimer disease, and the analysis of sialoforms of transferrin from human serum.

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Separation of complex mixtures of fluorobenzoic acids by capillary electrophoresis

Cited by 9 publications

References 22 publications

Polyelectrolyte Multilayers in Capillary Electrophoresis

Polyelectrolyte Multilayers in Capillary Electrophoresis

Rapid ion-exchange matrix removal for a decrease of detection limits in the analysis of salt-rich reservoir waters for fluorobenzoic acids by liquid chromatography coupled with tandem mass spectrometry

Contribution of CE to the Analysis of Protein or Peptide Biomarkers

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