Separation of conjugated linoleic acid isomers and parinaric fatty acid isomers by capillary electrophoresis

Öhman, Marcus; Wan, Hong; Hámberg, Mats; Blomberg, Lars G.

doi:10.1002/1615-9314(20020601)25:8<499::aid-jssc499>3.0.co;2-x

Cited by 16 publications

(7 citation statements)

References 34 publications

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“…Apart of the applications described above, CE methods have also been used to analyze other different compounds in foods [163][164][165][166][167][168][169][170][171][172][173][174]. Thus, capillary electromigration methods have successfully been applied for the simultaneous determination of multiple constituents in real beer samples [163,164].…”

Section: Other Applicationsmentioning

confidence: 99%

“…Some other interesting applications of CE in food analysis include authenticity studies of cheese [168], determination of taurine in Lycium Barbarum L., beverages and milk powder [169], separation of conjugated linoleic acid isomers and parinaric fatty acid isomers [170], determination of phytohormones in tomato [171] and coconut [172], separation and determination of active components (anthraquinone derivatives) in rhubarb [173] and separation and analysis of the major constituents (eugenol and β-caryophyllene) of cloves [174].…”

Section: Resistant Intermediate and Susceptible To Infection By Potamentioning

confidence: 99%

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Recent advances in the application of capillary electromigration methods for food analysis

García‐Cañas

Cifuentes

2007

Electrophoresis

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Section: Other Applicationsmentioning

confidence: 99%

Section: Resistant Intermediate and Susceptible To Infection By Potamentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis

García‐Cañas

Cifuentes

2007

Electrophoresis

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“…Following Hamberg [30] and Ohman et al [31], the third largest peak in Figure 6, peak 4, is assigned as the FAME of the tttc isomer of α-parinaric acid. The two smallest Hamberg [30] cautioned that thermal cis-to-trans isomerization can occur during GLC analysis of CSDA and estimated that it occurred to the extent of about 5% to 8% for a given CSDA isomer under the conditions used.…”

Section: :4 Isomersmentioning

confidence: 91%

“…Following Hamberg [30] and Ohman et al [31], the third largest peak in Figure 6, peak 4, is assigned as the FAME of the tttc isomer of α-parinaric acid. The two smallest peaks, peaks 3 and 5, are each about 1% of the CSDA FAME FID area.…”

Section: :4 Isomersmentioning

confidence: 91%

GC Analysis of Seven Seed Oils Containing Conjugated Fatty Acids

Goldschmidt

Byrdwell

2021

Separations

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The fatty acid compositions, including isomer compositions, of seven seed oils containing conjugated fatty acids (CFA) were determined. Seed oils were extracted using a modified Folch extraction, converted to fatty acid methyl esters, and analyzed by gas chromatography (GC) with mass spectrometry and flame ionization detection. The MS detector was operated in positive-ion chemical ionization mode using methane reagent gas. GC was performed using two columns providing different retention characteristics: a poly(ethylene glycol) column and a more polar biscyanopropyl column. The complimentary information provided by the two columns was crucial to peak identification in several cases. The major CFA species in the samples are well known but all contained lesser amounts of additional CFA that have not been widely reported. All samples contained multiple species of conjugated linolenic acid, and two samples also contained small amounts of conjugated linoleic acid. The seed oils of Jacaranda mimosifolia and Calendula officinalis were found to contain 8c,10t,12t-18:3, the natural occurrence of which has only been recently reported in some other samples. The seed oil of Impatiens balsamina has been reported to contain four conjugated 18:4 species, and we present evidence for a fifth conjugated 18:4 isomer.

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“…Additionally, soybean oils were investigated using similar methods . Finally, a comparison of the newly developed and the official GC methods was made , by way of the separation of C18:2 and C18:4 isomers with a minimum amount of MeOH (5–20%) and larger concentrations of surfactants, including SDS, (R)‐N‐dodecoxycarbonylvaline and CD, using direct UV detection . The isomers of C18:3 were also studied under very similar conditions , as were the cis‐trans isomers of linoleic acid .…”

Section: Free Fatty Acidsmentioning

confidence: 99%

A digest of capillary electrophoretic methods applied to lipid analyzes

Poinsot

Meang

et al. 2018

Electrophoresis

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Lipids are naturally occurring organic compounds that can be classified into a number of types based on their solubility in nonpolar organic solvents, and are generally insoluble in water. The great structural variety of these various types of lipids has led them to be components of many different biological substances such as oils, waxes, cellular membranes, tissues and biological fluids. The use of capillary electrophoresis (CE) for the study of lipids during the past 30 years has been relatively rare when compared to its use for other classes of biomolecules, primarily due to their insolubility in water. However, a number of interesting studies have been conducted, and as part of this review, we will present the different approaches that were used, which mainly consist of micellar kinetic chromatography and non-aqueous CE. The main advantages of the use of these techniques compared to GC is the very simple sample preparation that is required and, compared to LC, the very robust and quick separations that can be obtained. In this review, we present the various methods that have been reported in the literature that have been used for the study of fatty acids, phospholipids, glycerides, eicosanoids and sterols, with the inclusion of various tables presenting descriptions of the CE methods used as well as the methods of detection, including UV absorbance, fluorescence, mass spectrometry, and conductivity. This review aims to demonstrate that CE can be easily used for the analysis of lipids.

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Separation of conjugated linoleic acid isomers and parinaric fatty acid isomers by capillary electrophoresis

Cited by 16 publications

References 34 publications

Recent advances in the application of capillary electromigration methods for food analysis

Recent advances in the application of capillary electromigration methods for food analysis

GC Analysis of Seven Seed Oils Containing Conjugated Fatty Acids

A digest of capillary electrophoretic methods applied to lipid analyzes

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