Separation of Copolymers of Variable Compositions by Capillary Zone Electrophoresis

Peric, I. M.; Rivas, Bernabé L.; Pooley, Amalia; Rifffo, Elizardo; Basáez, Luís

doi:10.4067/s0366-16441999000300011

Cited by 4 publications

(11 citation statements)

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“…These results are in good accordance with previous works on the topic. [12][13][14] However, this study emphasizes that the separation of polyelectrolytes with different charge densities can be obtained, at least partially, on a large range of t values, even above t*. In this respect, counter-electro-osmotic migration is in favor of increasing the apparent selectivity of the separation for the highly charged polyelectrolytes.…”

Section: Resultsmentioning

confidence: 93%

“…The counterion condensation theory developed by Manning [10][11][12][13][14] states that, if the dimensionless charge density parameter (x) exceeds unity, there is a layer of counterion along the polyelectrolyte backbone which will effectively reduce x to unity (for monovalent counterions). The dimensionless charge density parameter x is defined as Equation (3).…”

Section: Counterion Condensationmentioning

confidence: 99%

“…[6][7][8][9] It is now well-known that free-solution capillary electrophoresis (FSCE) has the potential to separate polyelectrolytes according to their charge density, as far as this density is lower than the threshold value above which counterion condensation occurs. [10][11][12][13][14] The term ''free solution'' means that the separation is performed in the absence of a sieving matrix (gel or entangled polymer solution). Hoagland Summary: This work reports the separation of polyelectrolytes according to their charge density and their molecular mass by two-dimensional capillary electrophoresis (2D-CE).…”

Section: Introductionmentioning

confidence: 99%

“…This result was in good accordance with the threshold charge rate predicted by Manning's theory. [10][11][12][13][14] Above 35% (acrylic acid proportion), the electrophoretic mobility becomes almost independent of the chemical composition of the copolymer. Similar electrophoretic behavior was observed by Gao et al on random copolymer of 2-acrylamido-2-methylpropanesulfonate and acrylamide.…”

Section: Introductionmentioning

confidence: 99%

“…It is now well‐known that free‐solution capillary electrophoresis (FSCE) has the potential to separate polyelectrolytes according to their charge density, as far as this density is lower than the threshold value above which counterion condensation occurs 10–14. The term “free solution” means that the separation is performed in the absence of a sieving matrix (gel or entangled polymer solution).…”

Section: Introductionmentioning

confidence: 99%

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Charge‐ and Size‐Based Separations of Polyelectrolytes by Heart‐Cutting Two‐Dimensional Capillary Electrophoresis

Cottet

Biron

2005

Macro Chemistry & Physics

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Summary: This work reports the separation of polyelectrolytes according to their charge density and their molecular mass by two‐dimensional capillary electrophoresis (2D‐CE). The two dimensions of the separation are performed in the same capillary using a heart‐cutting approach. The first dimension of the separation is performed in free‐solution CE (i.e. in the absence of sieving matrix) and allows separating the polyelectrolytes according to their charge density. A selected fraction stemming from the first dimension is next submitted to a second separation medium that is introduced in the capillary by electro‐osmotic flow. The second dimension of the separation is performed in the presence of an entangled polymer solution and allows the size‐based separation of the polyelectrolytes. The interest of heart‐cutting 2D‐CE in comparison with one‐dimensional separations is clearly demonstrated using a model mixture containing ten synthetic polyelectrolytes. Good repeatability on migration times (RSD from 0.12% to 0.33%, calculated on 5 successive runs) and relative peak areas (RSD from 0.74% to 3.6%) was obtained using this new methodology.

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Section: Resultsmentioning

confidence: 93%

Section: Counterion Condensationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Charge‐ and Size‐Based Separations of Polyelectrolytes by Heart‐Cutting Two‐Dimensional Capillary Electrophoresis

Cottet

Biron

2005

Macro Chemistry & Physics

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Molecular Dynamics Simulations Illuminate the Role of Counterion Condensation in the Electrophoretic Transport of Homogalacturonans

et al. 2017

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Homogalacturonans (HGs) are polysaccharide copolymers of galacturonic acid and its methylesterified counterpart. The inter- and intramolecular distributions of the methylesterifed residues are vital behavior-determining characteristics of a sample's structure, and much experimental effort has been directed to their measurement. While many techniques are able to measure the sample-averaged degree of methylesterification (DM), the measurement of inter- and intramolecular charge distributions are challenging. Here, molecular dynamics (MD) simulations are used to calculate the electrophoretic mobilities of HGs that have different amounts and distributions of charges placed along the backbone. The simulations are shown to capture experimental results well, even for low-DM samples that possess high charge densities. In addition, they illuminate the role that local counterion condensation can play in the determination of the electrophoretic mobility of heterogeneous blocky polyelectrolytes that cannot be adequately described by a single chain-averaged charge spacing.

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Electrophoretic Behavior of Copolymeric Galacturonans Including Comments on the Information Content of the Intermolecular Charge Distribution

et al. 2009

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Capillary electrophoresis has been used to characterize samples of the copolymeric anionic polysaccharides homo- and rhamno-galacturonan (HG and RGI, respectively). In the case of HG, the ratios of the two component sugar residues, galacturonic acid and its methylesterified analogue, have been controlled chemically to produce samples comprised of varying degrees of methylesterification (DM). The mapping of the measured electrophoretic mobilities to the biopolymer charge density has been considered in some detail and for HG substrates with random intramolecular patterns of methylesterification it is shown that the experimentally extracted intermolecular DM distribution agrees well with the predictions of calculations based on the binomial theorem. This demonstrates that the intermolecular distribution of the methylesterification of pectin samples contains information on the intramolecular pattern by virtue of the fact that they are both manifestations of the same statistical process.

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Separation of Copolymers of Variable Compositions by Capillary Zone Electrophoresis

Cited by 4 publications

References 0 publications

Charge‐ and Size‐Based Separations of Polyelectrolytes by Heart‐Cutting Two‐Dimensional Capillary Electrophoresis

Charge‐ and Size‐Based Separations of Polyelectrolytes by Heart‐Cutting Two‐Dimensional Capillary Electrophoresis

Molecular Dynamics Simulations Illuminate the Role of Counterion Condensation in the Electrophoretic Transport of Homogalacturonans

Electrophoretic Behavior of Copolymeric Galacturonans Including Comments on the Information Content of the Intermolecular Charge Distribution

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