Separation of enantiomers of drugs by capillary electrophoresis

Separation of drug enantiomers by capillary electrophoresis in the presence of neutral cyclodextrins

“…The verification of that hypothesis is disof a bears the advantage of not being influenced by m played in Fig. 1 [13]. While all racemates not the peak shape, thus revealing the degree of enanresolved at 45 mM CSA were not influenced (Da 5 tiodiscrimination, regardless of the peak resolution m 0), most of those with strong retardation profited achieved.…”

“…Dependent on the availabilthere is a definite lack of publications that cover a ity and cost-effectiveness, asymmetric synthesis by either chemical catalysis or biotechnology are competing with preparative separation of the racemic Table 1 drug in a final stage. Separation science has taken an Methods used in capillary electrophoresis for the separation of important role in all stages of the process: Enantioenantiomers, as of Feb 1999, according to our database Chirbase / mer separation on an analytical scale is required for CE [14] monitoring the enantiomeric purity and for screening As a core part of this selected review, we report the analytical separation with capillary zone electroon our original data obtained for 86 drug racemates phoresis is now running through different stages of by CZE in the presence of heptakis(2,3,6-tri-Oan extensive screening process. This project has methyl)-b-cyclodextrin (TM-b-CD, also known as become feasible by the generous donation of fully permethyl-b-cyclodextrin) in the run buffer.…”

“…(Herfigures defined in our previous publications, i.e., the cules, CA,USA). Meanwhile, we have obtained a migration separation factor (a ) [11], and the m significant number of comparable data [10][11][12][13][14][15][16][17][18][19][20] for migration retardation factor (R ) [11] were comm neutral cyclodextrins, whereas the utility of charged pared with recently published data for native bcyclodextrins will be covered in a forthcoming stage cyclodextrin (b-CD) [12,17]. This comparison may of the project.…”

“…In a previous paper, we have shown that program, the applied conditions are relatively opno resolution was missed that way even though some timum for most analytes although they may be less racemates have a different optimum CD concenappropriate for a few analytes. Because of the tration [13]. A maximum difference in total mo-bilities of the enantiomers can be reached if about related to the ratio of electrophoretic mobilities (m), half of each enantiomer is bound to the CSA [28].…”

“…In Ref. [13], 23 selected analytes were measured with both 45 mM As emphasized previously, the electroosmotic flow and 30 mM HP-g-CD as the CSA. The lower (EOF) may be neglected under these conditions, concentration seemed close enough to the initial based on the observation that the neutral compound value of 45 mM to avoid the occurence of minima dimethylsulfoxide could not be detected within 2 h and maxima, respectively, of a between the two under the conditions applied.…”