Separation of Fatty Acid Ethanolamides using High Performance Liquid Chromatography

Suhendra, Dedy; Gunawan, Erin Ryantin; Astuti, Fuji; Kurniawati, Lely

doi:10.14233/ajchem.2020.22782

Cited by 2 publications

(3 citation statements)

References 13 publications

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“…A total of 15,401 mL of ethanolamine was measured with a volume pipette and was put into a 250 mL volumetric flask, 6 M HCl was added slowly until the pH was neutral. The mix was then diluted with distilled water (Suhendra et al, 2020).…”

Section: Preparation Of Standard Ethanolaminementioning

confidence: 99%

“…Alkylethanolamide was synthesized using previous research methods by Suhendra et al, (2020) that was conducted using lipozyme and 100 mL of the 7.5% (w/w) hexane solvent, C. inophylum oil 10 g, 50 mL of n-hexane, 125 mL of ethanolamine. The mixture was incubated in a horizontal water bath shaker at 150 rpm at a temperature of 40° C for 2 hours.…”

Section: Synthesis and Purification Of Alkyletanolamidementioning

confidence: 99%

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Application of Alkyletanolamide Surfactants Based on Nyamplung (Callophylum Inophylum L.) Seed Oil in Cream Base

Nurfalah,

Gunawan,

Suhendra

et al. 2023

fitofarmaka JIF

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Cream preparation is cosmetic preparation consisting of aqueous and oil phases that immiscible. Thus, a surfactant with hydrophilic and lipophilic groups is needed to reduce the interfacial tension between the two phases. Petroleum-based surfactants such as Triethanolamine (TEA) are often used in the cosmetic industry. In this study, however, alkyletanolamide surfactant synthesized from vegetable oil was used. Nyamplung, Callophylum inophylum L. (C. inophylum) oil is one of non-commercial vegetable oil that can be used as a substitute to produce surfactants. The aims of this study was to apply surfactant synthesized from C. inophylum oil on the cream preparation and to characterize the alkylethanolamide surfactant by determining the value of hydrophilic-lipophilic balance (HLB). Four different surfactant concentration, 1.5%, 2.0%, 2.5% and 0% were tested as base for the cream preparation. The best surfactant concentration for the cream-based formulation obtained in this study was 2.5% and the surfactant HLB value obtained was 14.40 indicating that alkyletanolamide surfactants can be applied to the oil-in-water (O/W) type of cream base formula.

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Section: Preparation Of Standard Ethanolaminementioning

confidence: 99%

Section: Synthesis and Purification Of Alkyletanolamidementioning

confidence: 99%

Application of Alkyletanolamide Surfactants Based on Nyamplung (Callophylum Inophylum L.) Seed Oil in Cream Base

Nurfalah,

Gunawan,

Suhendra

et al. 2023

fitofarmaka JIF

View full text Add to dashboard Cite

show abstract

“…Based on the chromatogram that has been compared with the standard, the composition of N-MFHAs is obtained, namely linoleoyl-MFHA (13.09%), oleoyl-MFHA (62.46%), palmitoyl-MFHA (19.51%) and stearoyl-MFHA (4.93%). The order of polarity follows: alkyne > alkene > alkane (Suhendra, et al, 2020). First, Linoleoyl-MFHA compounds were eluted and oleoyl-MFHA subsequently as the hydrocarbons polarities having single bonds are lower than those having double bonds.…”

Section: Separation N-mfhas Using Hplc Optimum Conditionsmentioning

confidence: 99%

Separation of N-Methylhydroxamic Fatty Acids Based on Ketapang Seed Oil using High Performance Liquid Chromatography

Nirwana¹,

Suhendra

Gunawan³

2022

jppipa, pendidikan ipa, fisika, biologi, kimia

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N-methyl fatty hydroxamic acids (N-MFHAs) is a derivative of a hydroxamic acid (FHA), were synthesized from ketapang seed oil (Terminalia catappa L.) with N-methyl hydroxylamine enzymatically using immobilized lipase (Lipozyme TL IM). N-MFHAs that are synthesized are still in a mixed state of triglycerides of the ketapang seed oil. This research aims to determine the optimum conditions for the separation of N-MFHAs into their single components and their percentage composition using High Performance Liquid Chromatography (HPLC). The specification of the HPLC system includes using the reverse phase column SGE C-18 ODS-2 and UV detector 213 nm. The optimum conditions for the separation of N-MFHAs in the HPLC system include using the mobile phase of methanol 100% and flow rate of 0.25 mL/minutes with an injection volume of 20 µL and sample concentration of 10.000 ppm. The percentage of the N-MFHAs composition successfully separated using HPLC were linoleoyl-methyl-fatty hydroxamic acid (13.09%), oleoyl-methyl-fatty hydroxamic acid (62.46%), palmitoyl-methyl-fatty hydroxamic acid (19.51%), and stearoyl-methyl-fatty hydroxamic acid (4.93%).

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Separation of Fatty Acid Ethanolamides using High Performance Liquid Chromatography

Cited by 2 publications

References 13 publications

Application of Alkyletanolamide Surfactants Based on Nyamplung (Callophylum Inophylum L.) Seed Oil in Cream Base

Application of Alkyletanolamide Surfactants Based on Nyamplung (Callophylum Inophylum L.) Seed Oil in Cream Base

Separation of N-Methylhydroxamic Fatty Acids Based on Ketapang Seed Oil using High Performance Liquid Chromatography

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