Separation of fluorescein dyes by capillary electrophoresis using β-cyclodextrin

Pérez-Ruíz, Tomás; Martı́nez-Lozano, Carmen; Sanz, Antonio; Bravo, E.

doi:10.1007/bf02467681

Cited by 14 publications

(4 citation statements)

References 11 publications

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“…With the above calculations, one can derive the mean electrophoretic mobility of fluorescein for the performed experiments applying eq , which results in µ f = (1.42 ± 0.1) × 10 −4 cm 2 (V s) −1 . This value is lower than in previous CE related publications; however, the values correspond to values obtained, e.g., by Fonslow et al with a μ-FFE device . In terms of voltage efficiency, as defined by the ratio of the utilized voltage for separation and the total applied voltage, the system suffers from the potential loss across the electrode regions.…”

Section: Resultssupporting

confidence: 47%

Bubble-Free Operation of a Microfluidic Free-Flow Electrophoresis Chip with Integrated Pt Electrodes

et al. 2008

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In order to ensure a stable and efficient separation in microfluidic free-flow electrophoresis (FFE) devices, various methods and chips have been presented until now. A major concern hereby is the generation of gas bubbles caused by electrolysis and the resulting disturbances in the position of the separated analyte lanes. Instable lane positions would lead to a decreased resolution in sample collection over time which certainly would be problematic when incorporating a stationary detector system. In contrast to our previous publications, in which we implemented laborious semipermeable membranes to keep bubbles outside the separation region, here we describe an electrochemical approach to suppress the electrolysis of water molecules and therefore bubble formation. This approach allowed a simpler and additionally a closed chip device with integrated platinum electrodes. With the use of this chip, the successful separation of three fluorescent compounds was demonstrated. Quinhydrone, which is a complex of hydroquinone and p-benzoquinone, was added only to the local flow streams along the electrodes, preventing mixing with the separation media and sample. The electrical current was generated via the oxidization and reduction of hydroquinone and p-benzoquinone up to a certain limit of the electrical current without gas formation. The separation stability was investigated for the chip with and without quinhydrone, and the results clearly indicated the improvement. In contrast to the device operating without quinhydrone, a 2.5-fold increase in resolution was achieved. Furthermore, separation was demonstrated within tens of milliseconds. This chemical approach with its high miniaturization possibilities offers an interesting alternative, in particular for low-current miniaturized FFE systems, in which large and open electrode reservoirs are not tolerable.

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Section: Resultssupporting

confidence: 47%

Bubble-Free Operation of a Microfluidic Free-Flow Electrophoresis Chip with Integrated Pt Electrodes

et al. 2008

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“…5.1 (16c;16f)). This value is lower than in previous CE related publications [104], however, correspond to values obtained e.g. by Fonslow et al with a µ-FFE device [35].…”

Section: Quantitative Bubble Influencesupporting

confidence: 72%

Microfluidic free-flow electrophoresis for proteomics-on-a-chip

Kohlheyer¹

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“…developed an HPLC coupled with fluorescence detection method to rapidly, simply, and sensitively determine rhodamine B in chili‐containing products. However, CE is superior to these methods, and it requires significantly less injection volume (nanoscale) compared to HPLC does (microscale) and saves organic reagents .…”

Section: Resultsmentioning

confidence: 99%

A simple and compact fluorescence detection system for capillary electrophoresis and its application to food analysis

Zhai

Yuan

et al. 2015

Electrophoresis

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A novel fluorescence detection system for CE was described and evaluated. Two miniature laser pointers were used as the excitation source. A Y-style optical fiber was used to transmit the excitation light and a four-branch optical fiber was used to collect the fluorescence. The optical fiber and optical filter were imported into a photomultiplier tube without any extra fixing device. A simplified PDMS detection cell was designed with guide channels through which the optical fibers were easily aligned to the detection window of separation capillary. According to different requirements, laser pointers and different filters were selected by simple switching and replacement. The fluorescence from four different directions was collected at the same detecting point. Thus, the sensitivity was enhanced without peak broadening. The fluorescence detection system was simple, compact, low-cost, and highly sensitive, with its functionality demonstrated by the separation and determination of red dyes and fluorescent whitening agents. The detection limit of rhodamine 6G was 7.7 nM (S/N = 3). The system was further applied to determine illegal food dyes. The CE system is potentially eligible for food safety analysis.

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Separation of fluorescein dyes by capillary electrophoresis using β-cyclodextrin

Cited by 14 publications

References 11 publications

Bubble-Free Operation of a Microfluidic Free-Flow Electrophoresis Chip with Integrated Pt Electrodes

Bubble-Free Operation of a Microfluidic Free-Flow Electrophoresis Chip with Integrated Pt Electrodes

Microfluidic free-flow electrophoresis for proteomics-on-a-chip

A simple and compact fluorescence detection system for capillary electrophoresis and its application to food analysis

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