Separation of inorganic anions on a triazole‐functionalized ion exchanger in ion chromatography

Abstract

The retention behavior of inorganic anions on a triazole-based stationary phase was first examined in ion chromatography. It was initially designed for hydrophilic interaction liquid chromatography and was simply prepared by introducing the triazole groups onto the surface of silica gel via click chemistry. Effective separation of common inorganic anions, including iodate, chloride, bromide, nitrate and iodide, was achieved with Na(2)SO(4) eluent. The logarithm of the retention factor of analytes was observed to be linear with the logarithm of the eluent concentration, and the slopes of the plots were almost the same as those of the ideal theoretical value. The eluent pH value in the range of 3.4-7.0 had little effect on the separation. The utility of the column was demonstrated for the determination of UV-absorbing anions in saliva and tap water.

Silica based click amino stationary phase for ion chromatography and hydrophilic interaction liquid chromatography

“…29 While its drawback was limited effective anion capacity (column, 4.6 mm i.d. Â 150 mm long, 2.67 mmol column À1 ).…”

“…As indicated previously, the protonated triazole ring or trapped eluent cation served to be ion exchange sites. 29 Herein the eluent of 5 mM Na 2 SO 4 + 0.5 mM H 2 SO 4 and 5 mM Na 2 SO 4 were used to evaluate the retention of the analytes, respectively, as provided in support information SI- Fig.3. † The results show that the retention of five model anions slightly increased with the decrease of the eluent pH value, indicating that the pH value of the eluent has less of an effect on the retention of the analytes.…”

“…In previous work, we have described a simple hydrophilic stationary phase bearing a 1,2,3-triazole ring and a hydroxymethyl group in 4-position of this ring, which could be used for separating polar compounds under HILIC mode as well as separating anions under IC mode. 18,29 The existence of 1,2,3-triazole ring plays a major role in the separation of inorganic anions. 29 A drawback of this stationary phase is the limited exchange capacity.…”

“…18,29 The existence of 1,2,3-triazole ring plays a major role in the separation of inorganic anions. 29 A drawback of this stationary phase is the limited exchange capacity. To address this, herein, a novel click triazole-amino stationary phase (CTASP) was designed and readily prepared via CuAAC reaction between the azide silica and 3-aminopropyne.…”

Preparation of hydrophilic polymer‐grafted polystyrene beads for hydrophilic interaction chromatography via surface‐initiated atom transfer radical polymerization

“…However, the triazole groups in the immobilized state have been proven to carry positive charge [31]. Recently, Zhang et al further confirmed this point by the experiment that a triazole-based stationary phase was used to separate inorganic anions by ion chromatography mode [32]. In this work, the z-potential values of poly(HTMA)-grafted beads (column C) were determined to be 11.4, 9.9, 8.3 and 4.7 mV in 20 mM ammonium formate at pH 3.0, 5.0, 7.0 and 8.0, respectively, whereas the z-potential of unmodified beads was in the range of À1.2 to À0.5 mV at the investigated pH range.…”