Separation of lactobacilli bacteriocins from fermented broths using membranes

Zacharof, Myrto-Panagiota; Coss, G.M; Mandale, Stephen; Lovitt, Robert W.

doi:10.1016/j.procbio.2013.05.017

Cited by 25 publications

(10 citation statements)

References 54 publications

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“…In addition, some recent studies have shown that the encapsulation of bacteriocins in nanoparticles enhanced the activity of these peptides against food spoiling microorganisms and multidrug resistant bacteria [ 79 , 80 ]. Moreover, the use of materials and/or methods based on nanotechnology in most cases showed a positive effect on the yield of bacteriocins, which facilitated their commercial production [ 81 ].…”

Section: Nanotechnologies In the Use Of Bacteriocinsmentioning

confidence: 99%

Overview of Global Trends in Classification, Methods of Preparation and Application of Bacteriocins

et al. 2020

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This paper summarizes information about the division of bacteriocins into classes (Gram-negative bacteria, Gram-positive bacteria, and archaea). Methods for producing bacteriocins have been studied. It is known that bacteriocins, most successfully used today are products of secondary metabolism of lactic acid bacteria. It is established that the main method of bacteriocin research is PCR analysis, which makes it possible to quickly and easily identify the presence of bacteriocin encoding genes. The mechanism of cytotoxic action of bacteriocins has been studied. It is proved that the study of cytotoxic (antitumor) activity in laboratory conditions will lead to the clinical use of bacteriocins for cancer treatment in the near future. It is established that the incorporation of bacteriocins into nanoparticles and targeted delivery to areas of infection may soon become an effective treatment method. The delivery of bacteriocins in a concentrated form, such as encapsulated in nanoparticles, will increase their effectiveness and minimize potential toxic side effects. The analysis of publications on this topic confirmed that diverse research on bacteriocins is relevant.

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Section: Nanotechnologies In the Use Of Bacteriocinsmentioning

confidence: 99%

Overview of Global Trends in Classification, Methods of Preparation and Application of Bacteriocins

et al. 2020

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“…Furthermore, some recent studies have shown that encapsulation of bacteriocins in nanoparticles has enhanced the activity of these peptides against food-spoiling microorganisms and multidrug-resistant bacteria (Arthur et al, 2014 ; Mossallam et al, 2014 ). In addition, the use of nanotechnology-based materials and/or methods has, in most cases, shown a positive impact on bacteriocin yield, thus facilitating their commercial production (Zacharof et al, 2013 ). Table 1 illustrates several examples of bacteriocins that have been formulated using nanotechnological approaches, while Figure 1 summarizes the major benefits form such formulations.…”

Section: Advantages Of Nanoformulated Bacteriocinsmentioning

confidence: 99%

Nanotechnology: A Valuable Strategy to Improve Bacteriocin Formulations

2016

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Bacteriocins are proteinaceous antibacterial compounds, produced by diverse bacteria, which have been successfully used as: (i) food biopreservative; (ii) anti-biofilm agents; and (iii) additives or alternatives to the currently existing antibiotics, to minimize the risk of emergence of resistant strains. However, there are several limitations that challenge the use of bacteriocins as biopreservatives/antibacterial agents. One of the most promising avenues to overcome these limitations is the use of nanoformulations. This review highlights the practical difficulties with using bacteriocins to control pathogenic microorganisms, and provides an overview on the role of nanotechnology in improving the antimicrobial activity and the physicochemical properties of these peptides.

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“…The degree of purification required is depending on its subsequent industrial applications; partial purification may be sufficient for an application in food industry. On the other hand, high purity (i.e., >99%) is required for pharmaceutical applications such as for stomach ulcers and colon infection treatment products, and for potential birth control . High purity is also required for a characterization study or in the study of the action mechanism of bacteriocins .…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, high purity (i.e., >99%) is required for pharmaceutical applications such as for stomach ulcers and colon infection treatment products, and for potential birth control. 6 High purity is also required for a characterization study or in the study of the action mechanism of bacteriocins. 2 Several studies have reported on the use of conventional methods for bacteriocin purification.…”

Section: Introductionmentioning

confidence: 99%

Purification of a Bacteriocin‐Like Inhibitory Substance Derived from Pediococcus acidilactici Kp10 by an Aqueous Micellar Two‐Phase System

Jamaluddin

Ariff

Wong

2018

Biotechnology Progress

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Aqueous micellar two‐phase system (AMTPS) is an extractive technique of biomolecule, where it is based on the differential partitioning behavior of biomolecule between a micelle‐rich and a micelle‐poor phase. In this study, an AMTPS composed of a nonionic surfactant, Triton X‐100 (TX‐100) was used for purifying a bacteriocin‐like inhibitory substance (BLIS) derived from Pediococcus acidilactici Kp10. The influences of the surfactant concentration and the effect of additives on the partitioning behavior and activity yield of the BLIS were investigated. The obtained coexistence curves showed that the mixtures of solutions composed of different surfactant concentrations (5–30% w/w) and 50% w/w crude load were able to separate into two phases at temperatures of above 60 °C. The optimum conditions for BLIS partitioning using the TX‐100‐based AMTPS were: TX‐100 concentration of 22.5% w/w, CFCS load of 50% w/w, incubation time of 30 min at 75 °C, and back‐extraction using acetone precipitation. This optimal partitioning resulted in an activity yield of 64.3% and a purification factor of 5.8. Moreover, the addition of several additives, such as sorbitol, KCl, dioctyl sulfosuccinate sodium salt, and Coomassie® Brilliant Blue, demonstrated no improvement in the BLIS separation, except for Amberlite® resin XAD‐4, where the activity yield was improved to 70.3% but the purification factor was reduced to 2.3. Results from this study have demonstrated the potential and applicability of TX‐100‐based AMTPS as a primary recovery method for the BLIS from a complex fermentation broth of P. acidilactici Kp10. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 35: e2719, 2019

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Separation of lactobacilli bacteriocins from fermented broths using membranes

Cited by 25 publications

References 54 publications

Overview of Global Trends in Classification, Methods of Preparation and Application of Bacteriocins

Overview of Global Trends in Classification, Methods of Preparation and Application of Bacteriocins

Nanotechnology: A Valuable Strategy to Improve Bacteriocin Formulations

Purification of a Bacteriocin‐Like Inhibitory Substance Derived from Pediococcus acidilactici Kp10 by an Aqueous Micellar Two‐Phase System

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